1999 Fiscal Year Final Research Report Summary
Mechanisms of Mitochondrial Cell Death Induced by Radiation
| Project/Area Number |
10671786
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
病態科学系歯学(含放射線系歯学)
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| Research Institution | National Institute of Radiological Science |
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Principal Investigator |
MAJIMA Hideyuki National Institute of Radiological Sciences, International Space Radiation Laboratory, Researcher, 国際宇宙放射線医学研究センター, 研究員 (60165701)
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| Co-Investigator(Kenkyū-buntansha) |
ANDO Koichi National Institute of Radiological Sciences, International Space Radiation Laboratory, Researcher, 国際宇宙放射線医学研究センター, 研究員 (00159526)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Radiation / Apoptosis / Mitochondria / Mechanisms of Cell Death / Free Radical / Sensitivity / Vitamin E |
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| Research Abstract |
Mitochondrial DNA codes 13 components of electron transport chain. Lacking of mtDNA abrogates the electron transport system. We hypothesize that mitochondria deficient in functional electron transport system can result in increased production of superoxide and thus more oxidative stress. Sensitivity to X-rays, clonogenicity, frequency of double strand breaks(DSB), chromosome aberration, free radical generation, apoptosis and oxidative damage markers were examined in 143B human oseteosarcoma cell line (Rho+) and mtDNA-less (Rho0) cells derived from this cell line. No significant difference was observed between Rho+ and Rho0 cells without X-rays exposure for frequency of DSB and chromosome aberration. Clonogenicity assay showed greater sensitivity in Rho0 cells compared to Rho+ cells exposed to X-rays. More DSBs, and more chromosome aberrations in terms of gap, translocation, and deletion were observed in Rho0 cells. Substitution of normal mitochondria including mitochondrial DNA resulted recover of X-ray sensitivity. These results suggest that Rho0 cells lacking mtDNA has more oxidative stress and higher sensitivity to X-rays. Furthermore, the chromosomal damage by irradiation was restored substantially by Vitamin E.
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[Journal Article] Theoretical strategy : How to make treatment planning for heavy ion-beam therapy?1999
Author(s)
Majima HJ, Suzuki M, Ando K, Sakata K, Suzuki N, Okada S, Suzuki S, Miyamoto T, Fujitaka K, Fujii H, Yamada S, Tsujii H, Urano M
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Journal Title
Jpn.J.Med.Phys. 19
Pages: 37-47
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Evidence for mRNA expression of Vascular Endothelial Growth Factor by X-ray irradiation in a Lung Squamous Carcinoma Cell Line.1998
Author(s)
Ando S, Nojima K, Majima H, Ishihara H, Suzuki M, Furusawa Y, Yamaguchi H, Koike S, Ando K, Yamauchi M, Kuriyama T
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Journal Title
Cancer Letter 132
Pages: 75-80
Description
「研究成果報告書概要(和文)」より
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