1999 Fiscal Year Final Research Report Summary
Effects of Stress on Brain Neuronal Network and Modification of Benzodiazepines and Nitrous Oxide
| Project/Area Number |
10671901
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Surgical dentistry
|
|---|
| Research Institution | Kyushu Dental College |
|---|
Principal Investigator |
KAWAHARA Hiroshi KYUSHU DENTAL COLLEGE, DEPARTMENT OF DENTAL ANESTHESIOLOGY, ASSISTANT PROFESSOR, 歯学部, 講師 (10186124)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SAKAMOTO Eiji KYUSHU DENTAL COLLEGE, DEPARTMENT OF DENTAL ANESTHESIOLOGY, INSTRUCTOR, 歯学部, 助手 (00295859)
NAKANISHI Osamu KYUSHU DENTAL COLLEGE, DEPARTMENT OF DENTAL ANESTHESIOLOGY, PROFESSOR, 歯学部, 教授 (50137345)
|
|---|
| Project Period (FY) |
1998 – 1999
|
| Keywords | MICRODIALYSIS / BENZODIAZEPINE ANXIOLYTICS / MIDAZOLAM / NORADRENERGIC NEURON / LOCUS COERULEUS / PREFRONTAL CORTEX / STRESS / AUTO RECEPTOR |
|---|
| Research Abstract |
Dual-probe microdialysis was used to investigate the release of noradrenaline in the medial prefrontal cortex (mPFC) during stress. Male albino rats of a Wistar-derived strain were used for the experiments. For that purpose a micro-dialysis probe was implanted in the vicinity of the locus coeruleus (LC) and a second probe was placed in the ipsilateral PFC. Receptor specific antagonists acting in the αィイD22ィエD2 adrenoceptor (idazoxane). GABAィイD2AィエD2 (bicuculline), GABAィイD2BィエD2 (CGP52432), acetylacholine (atropine), corticotropine releasing factor (CRF) (CP 154,526), NMDA glutamate (CPP), non-NMDA glutamate Receptor (DNQX) were infused into the LC by retrograde dialysis, whereas extracellular NA was recorded in the ipsilateral PFC. During infusion of the various compounds rat were gently handled for 10 min.
Infusion of idazoxane potentiates the handling-induced increase in the release of NA in the PFC. Infusion of atropine, bicuculline CGP52432 and DNQX were without effect in the handling response. Infusion of the non-peptide CRF antagonist CP 154,526 and CPP suppressed the simulation of NA during handling stress, and benzodiazepine anxiolytic (midazolam) also suppressed.
It is concluded that αィイD22ィエD2, NMDA glutamate and CRF receptors modify the handling stress response of LC neurons, and benzodiazepine anxiolytics also modify it. These data suggest no major role for GABAergic, nor cholinergic affernts to the LC in mediating the stress response.
|
Research Products
(10 results)
