1999 Fiscal Year Final Research Report Summary
Detection of a candidate gene related the tooth formation using an animal model for oral disease
| Project/Area Number |
10671955
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | Nihon University |
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Principal Investigator |
MAEDA Takahide Nihon University School of Dentistry at Matsudo, Pediatric Dentistry, Professor, 松戸歯学部, 教授 (70130599)
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| Co-Investigator(Kenkyū-buntansha) |
ASADA Yoshinobu Nihon University School of Dentistry at Matsudo, Pediatric Dentistry, Associate Professor, 松戸歯学部, 助教授 (20184145)
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| Project Period (FY) |
1998 – 1999
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| Keywords | EL mouse / absence of the third molar / linkage analysis |
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| Research Abstract |
It has been reported that EL mice had absence of all third molars, and there was no significant difference in that incidence between the sexes. A genetic factor affecting the appearance of absence of the third molars in EL mice was studied using two strains of mice and their genetic crosses : EL/sea mice, MSM/Msf mice which have no hypodontia, F1 hybrid, F2 intercross and backcross mice. Based on these genetic crosses, it was concluded that the appearance of absence of the third molars in strain EL mice was recessively influenced by autosomal genetic factor(s). To search the candidate linkages, individual genotyping of both 10 F2 intercross and 7 F2 backcross mice with absence of the third molars was performed using the 58 informative markers. A high score in the xィイD12ィエD1-test for F2 sample was found at D3Mit17, 38 and 194 on chromosome 3 (xィイD12ィエD1=22.6, p<0.0001), while a high score in the xィイD12ィエD1-test for N2 sample was found at D3Mit17, 38 and 194 on chromosome 3 (xィイD12ィエD1=3.2, p<0.05). Based on these results, we propose that a candiate gene for the cause of absence of the third molars in EL mice would be existed on the middle region (44 〜 49 cM) of mouse chromosome 3.
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