1999 Fiscal Year Final Research Report Summary
Study on the pathogenesis of periodontal disease in patients with Down's syndrome
| Project/Area Number |
10671966
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
NISHIMURA Fusanori Okayama University, Dental School Hospital, Assistant Professor, 歯学部・附属病院, 講師 (80208222)
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| Co-Investigator(Kenkyū-buntansha) |
TAKASHIBA Shogo Okayama University, Dental School, Assistant Professor, 歯学部, 助教授 (50226768)
KOKEGUCHI Susumu Okayama University, Dental School, Instructor, 歯学部, 助手 (10144776)
EGUSA Masahiko Okayama University, Dental School Hospital, Assistant Professor, 歯学部・附属病院, 講師 (90243485)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Down ; s Syndrome / Neutrophil function / Aging / c-fos / Regeneration / c-fos / 組織修復 |
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| Research Abstract |
Patients with Down's Syndrome have been reported to be accompanied by severe periodontal disease. To try to understand the underlying mechanisms behind this, we assessed neutrophil functions in patients with Down's Syndrome. Additionally, patients with Down ; s Syndrome are known to suffer accelerated aging. From the viewpoint of premature aging, we evaluated regenerative capacity of periodontal ligament cells in aged individuals. The results of the study are as follows.
(1) Neutrophil chemotaxis against FMLP,C5a and IL-8 was not impaired in patients with Down's Syndrome when compared with age-matched healthy subjects.
(2) Periodontal ligament cells obtained from aged subjects showed shorter replicative life span as compared with those from juvenile donors.
(3) Periodontal ligament cells from aged subjects showed less chemotactic responses to b-FGF as compared with those from juvenile donors.
(4) Cells reached fully senescence did not express c-fos.
(5) Although migrated cells expressed c-fos , there observed many cells which did not express c-fos in unmigrated cells.
These results suggest that c-fos is necessary for cell migration as well as cell proliferation, and failure to express c-fos may be one of the reasons for low regenerative capacity seen in aged subjects. We conclude that impaired regeneration rather than low neutrophil function may be involved in the high prevalence of periodontal disease seen in Down's Syndrome patients.
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[Publications] Arai, H., Nomura, Y., Kinoshita, M., Nishimura, F., Takigawa, M., Takahashi, K., Washio, N., Takashiba, S., Murayama, Y.: "The inhibition of DNA synthesis by prostaglandin E2 in human gingival fibroblasts is independent of the cyclic AMP-protein kinase A signal transduction pathway"Journal of Periodontal Research. 33. 33-39 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Ohyama, H., Matsushita, S., Kato, N., Nishimura, F., Oyaizu, K., Kokeguchi, S., Kurihara, H., Takashiba, S., Nishimura, Y., Murayama, Y.: "T cell responses to 53-kDa outer membrane protein of Porphyromonas gingivalis in humans with early-onset periodontitis"Human Immunology. 59. 635-643 (1998)
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[Publications] Sawa, T., Nishimura, F., Ohyama, H., Takahashi, K., Takashiba, S., Murayama, Y.: "In vitro induction of activation-induced cell death in lymphocytes from chronic periodontal lesion by exogeneous Fas-ligand"Infection and Immunity. 67. 1450-1454 (1999)
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[Publications] Katsuragi, K., Takashiba, S., Takahashi, K., Guo, S., Nishimura, F., Arai, H., Murayama, Y.: "Immunopathological findings of an early-onset periodontitis patient with decreased CD18 expression"Journal of the International Academy of Periodontology. 1. 35-44 (1999)
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[Publications] Asahara, Y., Nishimura, F., Arai, H., Kurihara, H., Takashiba, S., Murayama, Y.: "Chemotactic response of periodontal ligament cells decreases with donor age : association with reduced expression of c-fos"Oral Diseases. 5. 337-343 (1999)
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[Publications] Sawada, S., Kokeguchi, S., Nishimura, F., Takashiba, S., Murayama, Y.: "Phylogenetic characterization of Centipeda periodontii, Selenomonas sputigena and Selenomonas species by 16S rRNA gene sequence analysis"Microbios. 98. 133-140 (1999)
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[Publications] Naruishi, K., Takashiba, S., Chou, H-H., Arai, H., Nishimura, F., Murayama, Y.: "Role of soluble interleukin-6 receptor in inflamed gingiva for binding of interleukin-6 to gingival fibroblasts"Journal of Periodontal Research. 34. 296-300 (1999)
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[Publications] Sawada, K., Kokeguchi, S., Hongyo, H., Sawada, S., Miyamoto, M., Maeda, H., Nishimura, F., Takashiba, S., Murayama, Y.: "Identification of a novel insertion sequence specific for virulent strains of Porphyromonas gingivalis by subtractive hybridization"Infection and Immunity. 67. 5621-5625 (1999)
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[Publications] Yamada, H., Nishimura, F., Naruishi, K., Chou, H-H., Takashiba, S., Albright, GM., Nares, S., Iacopino, AM., Murayama, Y.: "Phenytoin and cyclosporin-A supress the expression of MMP-1, TIMP-1 and cathepsin L, but not that of cathepsin-B in cultured gingival fibroblasts"Journal of Periodontology. (in press). (1999)
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[Publications] Asahara, Y., Nishimura, F., Yamada, H., Naruishi, K., Kataoka, M., Kido, J., Nagata, T., Murayama, Y.: "Mast cells are not involved in cyclosporin-A-induced gingival hyperplasia. -A study with mast cell deficient mice-"Journal of Periodontology. (in press). (1999)
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