1999 Fiscal Year Final Research Report Summary
Studies on bone regeneration by application of osteoblast differentiation marker, Cbfal, gene products
| Project/Area Number |
10671967
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
MYOKAI Fumio Okayama University, Dental School, Assistant Professor, 歯学部, 助手 (50263588)
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| Co-Investigator(Kenkyū-buntansha) |
TAKASHIBA Shogo Okayama University, Dental School, Associate Professor, 歯学部, 助教授 (50226768)
MURAYAMA Yoji Okayama University, Dental School, Professor, 歯学部, 教授 (50029972)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Bone Regeneration / Cbfal / Gene Expression |
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| Research Abstract |
For achievement of periodontal tissue regeneration, it is needed to allow the regeneration related factors express in vivo. These factors must act as differentiation and proliferation factors specific for periodontal tissues, have to be elucidated their characters and functions. In this study, we assessed the gene expressions for candidates including Cbfal, and proposed the possibility to introduce gene into periodontal fibroblasts.
Experimental results for the specific points :
1) Gene expression of candidates during rat alveolar bone regeneration
We examined what gene was differentially expressed during alveolar bone healing following artificial defect. Reverse Northern analysis revealed that transcripts for Cbfal, BGP, TGF-β its receptor type III during wound healing. Moreover, unique genes were isolated from periodontal tissues during wound healing.
2) Gene transfer in cell in vitro
Human gingival fibroblasts were co-cultured with IL-1β introduced COS-1 cells. IL-8 mRNA was detected in gingival fibroblasts nearby the COS-1 cells. This result suggests possibility of gene transfer and its application in vivo.
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