• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1999 Fiscal Year Final Research Report Summary

STUDY ON BEHAVIOR OF VITAMIN E IN BIOLOGICAL MEMBRANE MODEL USING FLUORINE AS PROBE

Research Project

Project/Area Number 10672098
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 医薬分子機能学
Research InstitutionSetsunan University

Principal Investigator

KUMADAKI Itsumaro  Setsunan University, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (20057326)

Co-Investigator(Kenkyū-buntansha) OMOTE Masaaki  Setsunan University, Faculty of Pharmaceutical Sciences, Asistant, 薬学部, 助手 (90299032)
ANDO Akira  Setsunan University, Faculty of Pharmaceutical Sciences, Lecturer, 薬学部, 講師 (20138960)
Project Period (FY) 1998 – 1999
KeywordsVitamin E / fluorine / liposome / ィイD119ィエD1F-NMR / relaxation time / mobility / fatty acid / phosphatidylcholine
Research Abstract

Biological activities of vitamin E are believed to be based on stabilization of biological membranes due to its anti-oxidant activity. However, some recent researches suggested that its activities are also based on mechanical stabilization of membranes. We prepared tocopherol derivatives with fluorine substituents, and studied orientation and mobility of vitamin E using relaxation time of ィイD119ィエD1F-NMR. In this research, we introduced fluorine atoms to various positions of fatty acids. Using fluorines of these acids as probes, we studied property of liposome membrane, and found that fluorines on 2- to 10-positions of stearic acid had approximately same and very short relaxation times, while those at the 12-position had much longer and at the 18-position the longest relaxation times. These results show that the outer layer of the membrane is rather rigid than the inner layer. Addition of vitamin E to these liposomes resulted in the shortening of the relaxation times. These effects were stronger in the inner part of the membrane. This result supports that vitamin E stabilizes the membrane mechanically. Syntheses of phosphatidylcholine with fluorine atoms are in progress for detailed studies.

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] C.Gu, W. H, Wysoski, I.Kumadaki他2名: "Dissociative and Reactive Hyperthermal Ion-surface Collisions with Langmuir-Blodgett Films Terminated by CF_3(CH_2)_n-, n-Perfluoroalkyl and Alkyl Groups"J. Am. Chem. Soc.. 120. 10554-10562 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] C. Gu, W. H. Wysocki, A. Harada, H. Takaya, I. Kumadaki: "Research on Langmuir-Blodgett membrane using 18, 18, 18-trifluorostearic acid synthesizedin this project was published as follows : Dissociative and Reactive Hyperthermal Ion-Surface Collisions with Langmuir-Blodgett Films Terminated by CFィイD23ィエD2(CHィイD22ィエD2)ィイD2nィエD2-,n-Pefluoroakyl- or n-alkyl Groups"J. Am. Chem. Soc.. 120. 10554-10562 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2001-10-23  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi