• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1999 Fiscal Year Final Research Report Summary

Histamine Release Mechanism from ECL Cells of Gastric Mucosa via Guanylate Cyclase and its Related Pharmacological Features

Research Project

Project/Area Number 10672146
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用薬理学・医療系薬学
Research InstitutionChiba University

Principal Investigator

YANO Shingo  Chiba University, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (90009655)

Project Period (FY) 1998 – 1999
Keywordsgastric acid secretion / ECL cell / mast cell / nitric oxide / histamine release / guanylate cyclase / adenylate cyclase
Research Abstract

The theme of the present project has been focused on histamine release mechanism from ECL cells via guanylate cyclase. The results are summarized in the following six point :
1) Nitric oxide (NO) donors stimulated gastric acid secretion in the mouse isolated stomach preparation. The response to their low doses, but not to their high doses, was inhibited by famotidine. The NO donors produced histamine release from ECL cells. However, these drugs inhibited gastric acid secretion probably by a direct action on gastric parietal cells.
2) Acid secretory responses to cholinergic agents and gastrin like peptides were inhibited by treatment with NOS inhibitors. Therefore, histamine release from ECL cells by NO may be involved in these acid secretory responses.
3) Membrane permeable cAMP and cGMP produced histamine release from ECL cells. This finding suggests that guanylate cyclase, as well as adenylate cyclase, is associated with intracellular signaling of histamine release.
4) IBMX, a nonspecific phophodiesterase inhibitor, stimulated acid secretion, but zaprinast, a V-typed phosphodiesterase inhibitor, had no effect on acid secretion. V-typed phosphodiesterase may not play a significant role in histamine release from ECL cells.
5) Treatment with drugs which increase intracellular calcium concentration stimulated acid secretion. The weak secretory response was inhibited by famotidine, but the strong response was not blocked. This finding suggests that ECL cells have higher sensitivity to intracellular calcium concentration than parietal cells.
6) Both of endogenous NO and exogenous NO inhibited histamine release from ECL cells in mast cells. Mast cells was apparently different from ECL cells in histamine releasing mechanism.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] K.Hasebe: "Inhibitory effect of N^w-nitro-L-arginine on gastric secretion induced by secretagogues and vagal stimulation in the isolated stomach"Eur. J. Pharmacol.. 350. 229-236 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Horie: "Stimulatory effect of dibutyryl cyclic GMP on acid secretion in mouse isolated stomach and histamine release in gastric mucosal cells"J. Physiol. (Paris). 93. 1-5 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 内田勝幸: "再摂食・塩酸誘起幽門洞潰瘍発生要因とジエチルジチオカルバメート併用による悪化"実験潰瘍. 25. 167-170 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松本和枝: "胃粘膜アンモニア発生系と胃粘膜障害モデル実験ならびに各種抗潰瘍薬のウレアーゼ阻害作用"実験潰瘍. 25. 260-265 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大木康弘: "マウス摘出胃におけるムスカリン受容体刺激酸分泌反応に対する非選択的カチオンチャネル遮断薬の抑制作用"実験潰瘍. 26(2). 224-227 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 矢野眞吾: "薬物受容体研究の進歩と薬物療法 : ヒスタミン受容体"薬局. 50(5). 1343-1349 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] L.T.Yamamoto: "Opioid receptor agonistic characteristics of mitragynine pseudoindoxyl in comparison with mitragynine derived from Thai medicinal plant Mitragyna speciosa"General Pharmacology. 33. 73-81 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Watanabe: "Recent Advances in Pharmacological Research on Traditional Herbal Medicine (ed. by Watanabe H. et al.)"Harwood Academic Puablishers Inc., Amsterdam. 15 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K. Hasebe, H. Horie, S. Yano, K. Watanabe: "Inhibitory effect of NィイD1wィエD1-nitro-L-arginine on gastric secretion induced by secretagogues and vagal stimulation in the isolated stomach."Eur. J. Pharmacol.. 350. 229-236 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Horie, K. Hasebe, S. Tsuchiya, S. Yano, K. Watanabe: "Stimulatory effect of dibutyryl cyclic GMP on acid secretion in mouse isolated stomach and histamine release in gastric mucosal cells."J. Physiol. (Paris). 93. 1-5 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Uchida, Y. Kato, M. Takayama, S. Tsuchiya, S. Horie, S. Yano, K. Watanabe: "Causative factors on the formation of gastric antral ulcer in re-fed rats and aggravation of the antral ulcer by the combination with diethyldithiocarbamate."Ulcer Research. 25. 167-170 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Matsumoto, S. Tsuchiya, S. Horie, S. Yano, K. Watanabe: "Influence of gastric ammonia production on gastric antral ulcer model, and urease inhibitory effect of some anti-ulcerous drugs."Ulcer Research. 25. 260-265 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Oki, S. Horie, S. Tsuchiya, S. Yano, K. Watanabe: "Effects of non-selective cation channel blockers on gastric acid secretion via muscarinic receptors in the isolated mouse whole stomach."Ulcer Research. 26. 224-227 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Yano: "Progress in drug receptor studies and pharmacotherapeutics : histamine receptor"J. Practical Pharmacy. 50. 1343-1349 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] L. T. Yamamoto, S. Horie, H. Takayama, N. Aimi, S. Sakai, S. Yano, J. Shan, P. K. T. Pang, D. Ponglux, K. Watanbe: "Opioid receptor agonistic characteristics of mitragynine pseudoindoxyl in comparison with mitragynine derived from Thai medicinal plan Mitragyna speciosa."General Pharmacology. 33. 73-81 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2001-10-23  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi