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2000 Fiscal Year Final Research Report Summary

Secretion and regulation of endothelin and ALP from human synovial cells

Research Project

Project/Area Number 10672181
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionFukushima Medical University

Principal Investigator

YOSHIDA Hiroshi  Medicine, Clin Lab., Prof., 医学部, 教授 (90045683)

Co-Investigator(Kenkyū-buntansha) IMAFUKU Yuji  Medicine, Clin Lab., associate, 医学部, 助手 (40254015)
Project Period (FY) 1998 – 2000
Keywordssynovial cells / endothelin / ALP / cytokines / 接着分子
Research Abstract

Macrophage-like type A synoviocytes collected from RA patients were shown to secrete endothelin (ET), alkaline phosphatase (ALP) and lactic dehydrogenase.
ET-1 is secreted from macrophage-like synoviocytes, but not from fibroblast-like type B cells, RA synovial fluid cells aud 7 established human cells. Analyses by HPLC disclosed that ET-1 is the major ET in synovial fluid.(CCA 1997, ACB 1998, APCB 1998).
ALP was secreted only from type A cells, of which properties were as follows ; α-β mobility, neuraminidase sensitive, heat-stable at 56℃ for 10-30 min, slightly inhibited by L-phenylalanine and high affinity to wheat germ lectin (90%).(16th IFCC, 1998)
Morphological studies by microscopy showed that ALP was stained granular in cytoplasma and by flow cytometry CD13 was positive and CD15 and CD11b were weakly positive.
Among 24 cytokines and 5 adhesion molecules tested, MMP2, IL6. IL8 and tissue factor were detected in the cultured supernatent.(ASCP 2000, WASPAL 2001).

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Yoshida,H. et al: "Cytokine production by human synoviacytes."GCK Asian Conf Clin Pathol (Abst). 156 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshida,H. et al: "Endothelin-1 production by human synoviacytes."Ann Clin Biochem. 35. 290-294 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshida,H. et al: "Production of endothelin-1 by cultured synoviacytes."Clin Chim Acta. 259. 187-189 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwafuku,Y. et: "The effect of detergents on chemical occult blood test."Fukashima J Med Sci. 45. 117-123 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ozawa,Y. et: "Determination of Kflwx activity of erythrocytes"Clin Chim Acta. 279. 125-132 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshida,H. et al: "Endo.thelin concentrations in synovial fluid and sera…"Asia Pacific.Clin.Biochem. 172 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshida, H.et al.: "Cytokine production by human synoviocytes"6th ACCP. 156. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida, H.et al.: "Endothelin-1 production by human synoviocytes."Ann.Clin.Biocheem.. 5. 290-294 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida, H.et al.: "Production of endothelin-1 by cultured synoviocytes."Clin Chim Acta. 259. 187-189 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida, H.et al.: "Endothelin concentrations in synovial fluids and sera of collagen diseases patients."APCB. 172. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Imafuku, Y.et al.: "The effect of detergents on chemical occult blood tests."Fukushima J Med Sci.. 45. 117-123 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ozawa, Y.et al.: "Determination of K-flux activity of erythrocytes."Clin Chim Acta. 279. 125-132 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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