2000 Fiscal Year Final Research Report Summary
Effect of Transformation of Plants on Protein Expression
| Project/Area Number |
10680141
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
食生活
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| Research Institution | Ochanomizu University |
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Principal Investigator |
MURATA Masatsune OCHANOMIZU UNIVERSITY, DEPARTMENT OF NUTIRTION AND FOOD SCIENCE, ASSOCIATE PROFESSOR, 人間文化研究科, 助教授 (60210051)
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| Co-Investigator(Kenkyū-buntansha) |
HONNMA Seiichi OCHNOMIZU UNIVERSITY, DEPARTMENT OF NUTIRTION AND FOOD SCIENCE, PROFESSOR, 生活科学部, 教授 (50017240)
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| Project Period (FY) |
1998 – 2000
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| Keywords | transgenic plant / protein expression / Agrobacterium / tobacco / apple / phenolic |
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| Research Abstract |
One of the issues on genetically modified foods is that unexpected or unintended proteins or metabolites will occur as a result of the genetic modification of crops. Here we have prepared transgenic tobacco and apple shoots as model plants and analyze the proteins and phenolics to give a fundamental knowledge on the safety of the genetically modified foods An antibiotic-resistant gene was used as a maker for the selection of transgenic plants. Beta-glucuronidase gene was used to colorimetrically examine the expression of inserted gene. Proteins and phenolics of transgenic shoots were compared with those of non-transgenic shoots. Proteins and phenolics were analyzed by SDS-PAGE and HPLC, respectively. Consequently, there were no significant differences between them.
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