1999 Fiscal Year Final Research Report Summary
Effect of spin trap agent under oxidative stress by high-dose oxygen exposure
| Project/Area Number |
10680520
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
環境影響評価(含放射線生物学)
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| Research Institution | University of Shizuoka |
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Principal Investigator |
SAITO Kieko Institute for Environmental sciences, 環境科学研究所, 助手 (10162190)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIOKA Hisashi Institute for Environmental sciences, 環境科学研究所, 助教授 (90046300)
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| Project Period (FY) |
1998 – 1999
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| Keywords | PBN / spin trapping / oxidative stress / nitric oxide / high pressure oxygen / ESR / Free radical |
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| Research Abstract |
We have been suggested that spin trap agent, N-tert-buttyl-α-Phenylnitrone (PBN) may be a source of nitric oxide other than serving an antioxidant under oxidative stress. In this study we determined the nitric oxide (NO) formation to characterize the effect of PBN under high pressure oxygen. NO formation of mice treated with high pressure oxygen was measured using an Nox analyzer and an electron spin resonance (ESR). An oxidative product of No, nitrite formation of the blood of mice under high pressure atmosphere. NOS (nitric oxide synthase) inhibitor, L-NAME, decreased the NO formation of the mice to the normal level of NO, indicating that the NO was formed via NOS. ESR spectra of liver and brain of the mice were measured inn the both control and experimental mice using Fe(DETC)2 as a NO trapper. Signal of NO complex was observed in the liver of the mice under the condition of high pressure oxygen. Furthermore, effect of PBN administration was investigated. Nitrite formation in the blood of the mice treated with PBN was decreased under the condition of high pressure oxygen. In addition, the intensity of NO complex was weaker in the PBN-treated mice than the non-treated mice on ESR study.
The results indicate that PBN suppressed NO formation under high pressure oxygen. PBN as a source of nitric oxide may control physiological functions under the condition of high-dose oxygen.
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