Research of egg specific glycosphingolipids-binding protein

1999 Fiscal Year Final Research Report Summary

• Project/Area Number: 10680563
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioorganic chemistry
• Research Institution: Niigata University
• Principal Investigator: ARAKI Shigeko Brain Research Institute, Niigata University Research Associate, 脳研究所, 助手 (70018604)
• Co-Investigator(Kenkyū-buntansha): ABE Sachiko Brain Research Institute, Niigata University Research Associate, 脳研究所, 助手 (60018603)
• Project Period (FY): 1998 – 1999
• Keywords: structural analysis / Aplysia / phosphonoglycosphingolipid / egg mass / lectin

Research Abstract

We isolated a novel glycolipid, EGL-II, in the egg of Aplysia kurodai and identified its structure as follows.
(1) Amino acid analysis showed the presence of 2-AEPn and glucosamine in a molar ratio of 3 : 1.
(2) The major aliphatic components of ceramide were palmitic acid, stearic acid and anteisononadeca-4-sphingenine.
(3) EGL-II was composed of glucose/galactose/N-acetylglucosamine/3-O-methylgalactose in a molar ratio of 1 : 5 : 1 : 1 and 2-AEPn was attached to each of 2 mol of galactose and 1 mol of glucose.
(4) From the results of the permethylation studies and the fragmentation diagram of the FAB-MS and SIMS spectra, the sugar sequence and linkage of EGL-II was concluded to be.
<<chemical formula>>
Gal1-3(GlcNAc1-2)Gal1-3(3-O-MeGal1-2)Gal1-3(Gal1-2)Gal1-4Glc1--Cer
(5) The proton magnetic resonance data revealed β-Glc, β-Gal, α-GlcNAc, α-3-O-MeGal, and four α-Gal protons in the anomeric region.