1999 Fiscal Year Final Research Report Summary
Analysis of cell proliferation by GM-CSF
| Project/Area Number |
10680597
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
WATANABE Sumiko Institute of Medical Science, Tokyo University, Department of Molecular and Developmental Biology, Research Associate, 医科学研究所, 助手 (60240735)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Cytokine / Receptor / Cell proliferation / GM-CSF |
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| Research Abstract |
GM-CSF regulates proliferation and differentiation of various hematopoietic cells. We have been analyzing signal transduction of human GM-CSF rcceptor and found that there are multiple distinct signaling pathways through human GM-CSF receptor. By using mutant receptors, we found that we can activate or inactivate certain signaling pathway. In this study, we tried to analyze signals for cell proliferation using the mutant hGM-CSF receptors which can activate proliferation without activation of MAPK cascade. We clones several genes by subtraction but we found difficulty to reveal function of these new genes since the cloning methods was non-functional. To clone and analyze genes by biochemical methods, we tried to establish in vitro reconstitution system of signaling pathway by fractionated cell lysates. We succeeded to make an in vitro system of STAT5 activation from hematopoietic cell lysate, but failed to apply this system to c-myc transcriptional induction. We cloned hGM-CSSF receptor binding proteins by pull-down analysis, and analyzed function of these cloned genes by STAT5 in vitro system.
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[Publications] Yamashita, Y., Watanabe, S., Miyazato, A., Ohya K., Ikeda U., Shimada, K., Komatsu, N., Hatake, K., Miura, Y., Ozawa, K., Mano, H.: "Tec and Jak2 kinases cooperate to mediate cytokine-driven activation of c-fos transcription."Blood. 91. 1496-1507 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yang, F-C., Watanabe, S., Tsuji, K., Xu, M.-J., Kaneko, A., Ebihara, Y., Nakahata, T.: "Human G-CSF stimulates the development of primitive multipotential progenitors of human G-CSF receptor-transgenic mice, but does not affect their commitment I vitro and in vivo"Blood. 92. 4632-4640 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Mukasa, R., Homma, T., Ohtsuki T., Hosono, O., Souta, A., Kitamura, T., Fukuda, M., Watanabe, S., Morimoto, C.: "Core 2-containin O-glycans on CD43 are preferentially expressed in the memory subset of human CD4 T cells."Int. Immunol.. 11. 259-268 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Yang, C.-F., Tsuji, K., Oda, A., Ebihara, Y., Xu, M.-J., Kaneko, A., Hanada, S., Mitsui, T., Kikuchi, A., Manabe, A., Watanabe, S., Ikeda, Y., Nakahata, T.: "Differential effects hG-CSF and thrombopoietin on megakaryopoiesis and platelet function in hG-CSF receptor-transgenic mice."Blood. 94. 950-958 (1999)
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