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1999 Fiscal Year Final Research Report Summary

Dynamic organization of chromosomal DNA in Escherichia coli.

Research Project

Project/Area Number 10680655
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionKUMAMOTO UNIVERSITY

Principal Investigator

NIKI Hironori  SCHOOL OF MEDICINE, KUMAMOTO UNIVERSITY, LECTURER, 医学部, 講師 (70208122)

Co-Investigator(Kenkyū-buntansha) 仁木 宏典  熊本大学, 医学部, 講師 (70208122)
Project Period (FY) 1998 – 1999
KeywordsProkaryote / cell division / FISH / nucleoid / partitioning / segregation
Research Abstract

We have revealed the subcellular localization of different DNA segments that are located at approximately 〜230-kb intervals on the Escherichia coli chromosome using fluorescence in situ hybridization (FISH). The series of chromosome segments is localized within the cell in the same order as the chromosome map. The large chromosome region including oriC shows similar localization patterns, which we call the Ori domain. In addition, the localization pattern of the large segment including dif is characteristic of the replication terminus region. The segment also shows similar localization patterns, which we call the Ter domain. In newborn cells, Ori and Ter domains of the chromosome are differentially localized near opposite cell poles. Subsequently, in the B period, the Ori domain moves toward mid-cell before the initiation of replication, and the Ter domain tends to relocate at mid-cell. An inversion mutant, in which the Ter domain is located close to oriC, shows abnormal subcellular localization of ori and dif segments, resulting in frequent production of anucleate cells. These studies thus suggest that the E. coli chromosome is organized to form a compacted ring structure with the Ori and Ter domains; these domains participate in the cell cycle- dependent localization of the chromosome.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Niki, H.: "Polar localization of the replication origin and terminus in Escherichia coli nucleoids during chromosome partitioning"Genes & Development. 12. 1036-1045 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiraga, S.: "Cell cycle-dependent duplication and bidirectional migration of SeqA-associated DNA-protein complexes in E. coli."Mol. Cell. 1. 381-387 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirano, M.: "Autoregulation of the partition genes of mini-F plasmid and the intracellular localization of their products in escherichia coli"Mol. Gen. Genet.. 257. 392-403 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Onogi, T.: "The assembly and migration of SeqA-Gfp fusion in living cells of Escherichia coli"Mol. Microbiol.. 31. 1775-1782 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamazoe, M.: "Complex formation of MukB, MukE and MukF proteins involved in chromosome partitioning in Escherichia coli"EMBO J.. 18. 5873-5884 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Niki, H.: "Subcellular localization of plasmids containing the oriC region of Escherichia coli chromosome, with or without the sopABC partitioning system"Mol. Microbiol.. 34. 498-503 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Niki, H.: "Dynamic organization of chromosomal DNA in Escherichia coli"Genes & Development. 14. 212-223 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 仁木 宏典: "細菌における分子細胞生物学の新展開ー染色体分配と細胞分裂"蛋白質核酸酵素. 45. 153-163 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Niki,H. and S.Hiraga: "Polar localization of the replication origin and terminus in Escherichia coli nucleoids during chromosome partitioning"Genes Dev. 12. 1036-1045 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirano,M., H.Mori, T.Onogi, M.Yamazoe, H.Niki, T.Ogura and S.Hiraga: "Autoregulation of the partition genes of the mini-F plasmid and the intracellular localization of their products in Escherichia coli"Mol Gen Genet. 257. 392-403 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiraga,S., C.Ichinose, H.Niki and M.Yamazoe: "Cell cycle-dependent duplication and bidirectional migration of SeqA-associated DNA-protein complexes in E. coli"Mol Cell. 1. 381-387 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Onogi,T., H.Niki, M.Yamazoe and S.Hiraga: "The assembly and migration of SeqA-Gfp fusion in living cells of Escherichia coli"Mol Microbiol. 31. 1775-1782 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamazoe,M., T.Onogi, Y.Sunako, H.Niki, K.Yamanaka, T.Ichimura and S.Hiraga: "Complex formation of MukB, MukE and MukF proteins involved in chromosome partitioning in Escherichia coli"EMBO J. 18. 5873-5884 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Niki,H. and S.Hiraga: "Subcellular localization of plasmids containing the oriC region of the Escherichia coli chromosome, with or without the sopABC partitioning system"Mol Microbiol. 34. 498-503 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Niki,H., Y.Yamaichi and S.Hiraga: "Dynamic organization of chromosomal DNA in Escherichia coli"Genes Dev. 14. 212-223 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Niki,H., Moriya,S. and S.Hiraga: "Progressing molecular cell biology in bacteria: chromosome partitioning nand cell division"Tanpakusitu Kakusan Koso. 45. 153-163 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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