1999 Fiscal Year Annual Research Report

侵害情報受容系とその調節系のニューロン連絡、特にペプチドとその受容体との関連

Research Project

Project/Area Number	10680701
Research Institution	TOKYO METROPOLITAN ORGANIZATION FOR MEDICAL RESEARCH
Principal Investigator	水野昇財団法人東京都医学研究機構, 東京都神経科学総合研究所, 所長 (10025596)
Keywords	三叉神経脊髄路核 / 膠様質 / P物質受容体(NK-1) / プレプロダイノルフィン / カルシウム結合蛋白質 / 痛覚 / 免疫組織化学 / スライス実験
Research Abstract	1.視床に投射線維を送る三叉神経脊髄路核尾側亜核ニューロンのなかにプレプロダイノルフィン様免活性を示すものがあることをラットを用いて証明した。 2.ヒト新生児および成人の脊髄について、P物質受容体様免疫活性を示すニューロンの分布を調べた。 3.スライス実験により、ラットの三叉神経脊髄路核尾側亜核II層(膠様質)のニューロンの膜特性と形態を細胞電位内記録法と細胞内標識法により探索した。投射ニューロンのほか、局所回路ニューロンを2型に分類した。 4.ラットのスライス実験により、三叉神経脊髄路核尾側亜核II層(膠様質)において、一群の局所回路ニューロンがカルビンディンD28K様免疫活性を示すことを発見した。 5.結合腕傍核、視床、ないし視床下部に投射線維を送る三叉神経脊髄路核尾側亜核ニューロンのなかに、パルブアルブミン様、カルレチニン様、ないしカルビンディンD28K様免疫活性を示すものがあること、また、これらのなかに、痛覚刺激を与えると、c-Fos様免疫活性を発現するものがあることをラットで証明した。

Research Products

(7 results)

All Other

All Publications (7 results)

[Publications] 李金連他3名: "Preprodynorphin-like immunoreactivity in medullary dorsal horn neurons projecting to the thalamic regions in the rat"Neuroscience Letters. 264・1, 2, 3. 13-16 (1999)
[Publications] 丁玉強他12名: "The distribution of substance P receptor (NK1)-like immunoreactive neurons in the newborn and adult human spinal cord"Neuroscience Letters. 266・2. 133-136 (1999)
[Publications] 李雲慶他3名: "Substantia gelatinosa neurons in the medullary dorsal horn : an intracellular labeling study in the rat"The Journal of Comparative Neurology. 411・3. 399-412 (1999)
[Publications] 李雲慶他3名: "Local circuit neurons showing calibindin D28k-immunoreactivity in the substantia gelatinosa of the medullary dorsal horn of the rat. An immunohistochemical study combined with intracellular staining in slice preparation"Brain Research. 840・1, 2. 179-183 (1999)
[Publications] 李金連他4名: "Calcium-binding protein-immunoreactive projection neurons in the caudal subnucleus of the spinal trigeminal nucleus of the rat"Neuroscience Research. 35・3. 225-240 (1999)
[Publications] 李金連他5名: "The relationship between neurokinin-1 receptor and substance P in the striatum : light and electron microscopic immunohistochemical study in the rat"The Journal of Comparative Neurology. (印刷中). (2000)
[Publications] 李金連他5名: "The relationship between neurokinin-1 receptor and substance P in the medullary dorsal horn. A light and electron microscopic immunohistochemical study in the rat"Neuroscience Research. (印刷中). (2000)

1999 Fiscal Year Annual Research Report

侵害情報受容系とその調節系のニューロン連絡、特にペプチドとその受容体との関連

Principal Investigator

水野 昇 財団法人 東京都医学研究機構, 東京都神経科学総合研究所, 所長 (10025596)

Research Products

[Publications] 李金連 他3名: "Preprodynorphin-like immunoreactivity in medullary dorsal horn neurons projecting to the thalamic regions in the rat"Neuroscience Letters. 264・1, 2, 3. 13-16 (1999)

[Publications] 丁玉強 他12名: "The distribution of substance P receptor (NK1)-like immunoreactive neurons in the newborn and adult human spinal cord"Neuroscience Letters. 266・2. 133-136 (1999)

[Publications] 李雲慶 他3名: "Substantia gelatinosa neurons in the medullary dorsal horn : an intracellular labeling study in the rat"The Journal of Comparative Neurology. 411・3. 399-412 (1999)

[Publications] 李雲慶 他3名: "Local circuit neurons showing calibindin D28k-immunoreactivity in the substantia gelatinosa of the medullary dorsal horn of the rat. An immunohistochemical study combined with intracellular staining in slice preparation"Brain Research. 840・1, 2. 179-183 (1999)

[Publications] 李金連 他4名: "Calcium-binding protein-immunoreactive projection neurons in the caudal subnucleus of the spinal trigeminal nucleus of the rat"Neuroscience Research. 35・3. 225-240 (1999)

[Publications] 李金連 他5名: "The relationship between neurokinin-1 receptor and substance P in the striatum : light and electron microscopic immunohistochemical study in the rat"The Journal of Comparative Neurology. (印刷中). (2000)

[Publications] 李金連 他5名: "The relationship between neurokinin-1 receptor and substance P in the medullary dorsal horn. A light and electron microscopic immunohistochemical study in the rat"Neuroscience Research. (印刷中). (2000)

水野昇財団法人東京都医学研究機構, 東京都神経科学総合研究所, 所長 (10025596)

[Publications] 李金連他3名: "Preprodynorphin-like immunoreactivity in medullary dorsal horn neurons projecting to the thalamic regions in the rat"Neuroscience Letters. 264・1, 2, 3. 13-16 (1999)

[Publications] 丁玉強他12名: "The distribution of substance P receptor (NK1)-like immunoreactive neurons in the newborn and adult human spinal cord"Neuroscience Letters. 266・2. 133-136 (1999)

[Publications] 李雲慶他3名: "Substantia gelatinosa neurons in the medullary dorsal horn : an intracellular labeling study in the rat"The Journal of Comparative Neurology. 411・3. 399-412 (1999)

[Publications] 李雲慶他3名: "Local circuit neurons showing calibindin D28k-immunoreactivity in the substantia gelatinosa of the medullary dorsal horn of the rat. An immunohistochemical study combined with intracellular staining in slice preparation"Brain Research. 840・1, 2. 179-183 (1999)

[Publications] 李金連他4名: "Calcium-binding protein-immunoreactive projection neurons in the caudal subnucleus of the spinal trigeminal nucleus of the rat"Neuroscience Research. 35・3. 225-240 (1999)

[Publications] 李金連他5名: "The relationship between neurokinin-1 receptor and substance P in the striatum : light and electron microscopic immunohistochemical study in the rat"The Journal of Comparative Neurology. (印刷中). (2000)

[Publications] 李金連他5名: "The relationship between neurokinin-1 receptor and substance P in the medullary dorsal horn. A light and electron microscopic immunohistochemical study in the rat"Neuroscience Research. (印刷中). (2000)