1999 Fiscal Year Annual Research Report

PACAP受容体のバソプレシン分泌・合成制御機構

Research Project

Project/Area Number	10680708
Research Institution	Showa University
Principal Investigator	塩田清二昭和大学, 医学部, 教授 (80102375)
Co-Investigator(Kenkyū-buntansha)	八木健国立岡崎共同研究機構, 生理学研究所, 助教授 (10241241) 矢田俊彦鹿児島大学, 医学部, 助教授 (60166527) 中条茂男昭和大学, 薬学部, 助教授 (50119236)
Keywords	PACAP / バソプレシン / シグナル伝達 / 視床下部 / ラット
Research Abstract	新規神経ペプチドPACAPの含有ニューロン終末は視床下部の視索上核や室傍核に密に分布し、その神経終末が神経内分泌細胞にシナプスを形成しているのを我々は免疫電顕的に観察・報告している。免疫組織化学法とIn situハイブリダイゼーション法の組み合わせ法を用いて視索上核をしらべたところ、バソプレシンニューロンが特異的にこのレセプターを発現しているのが明らかになった。バソプレシンニューロンはノルアドレナリン性ニューロンの神経支配も受けており、ノルアドレナリンおよびPACAPはcAMP-プロテインキナーゼA系を介して細胞内のカルシウム濃度を上昇させることが単離神経分泌細胞を用いた実験で明らかになった(Shioda et al.,1998,1999)。特異的なカルシウムチャンネル阻害剤を用いて上記と同様の実験を行った結果、PACAP/ノルアドレナリンは共調して細胞膜のT-,L-型のカルシウムチャネルをリン酸化し、細胞内に大量のカルシウムを流入させ、最終的に分泌果粒の放出が起きることが明らかになった(Shioda et al.,1999)。

Research Products

(12 results)

All Other

All Publications (12 results)

[Publications] H. Ozawa: "Delayed neuronal cell death in the rat hippocampus is mediated by the mitogen-activated protein kinase signal transduction pathway."Neurosci Lett. 262. 57-60 (1999)
[Publications] K. Ito: "Bioluminescent enzyme immunoassay for pituitary adenylate cyclase activating polypeptide 38(PACAP38)"Analytica Chimica Acta. 382. 245-251 (1999)
[Publications] H. Funahashi: "The effect of leptin on feeding-regulating neurons in the hypothalamus."Neurosci Lett. 264. 117-120 (1999)
[Publications] S. Muroya: "Glucose-sensitive neurons in the rat arcuate nucleus contain neuropeptide Y."Neurosci Lett. 264. 113-116 (1999)
[Publications] C. J. Zhou: "Ontogenetic expression of PACAP receptor in the rat brain: early in the neural plate at primitive stage."Neuroscience. 93. 375-391 (1999)
[Publications] H. Mizushima: "Effect of cardiac arrest on the permeability of the blood-brain and blood-spinal cord barrier to albumin and tumor necrosis factor-a."Life Sci. 65. 2127-2134 (1999)
[Publications] H. Mizushima: "The effect of cardiac arrest on the permeability of the mouse blood-brain and blood-spinal cord barriers to pituitary adenylate cyclase activating polypeptide(PACAP)."Peptides. 20. 1337-1340 (1999)
[Publications] M. Nakata: "Insulinotropin PACAP potentiates insulin-stimulated glucose uptake in 3T3-L1 cells."Peptides. 20. 943-948 (1999)
[Publications] C. J. Zhou: "Cellular distribution of the splice variants of the receptor for pituitary adenylate cyclase-activating polypeptide(PAC1-R) in the rat brain by in situ RT-PCR."Mol Brain Res. 95. 150-158 (2000)
[Publications] T. Seki: "The distribution and ultrastructural localization of Pituitary Adenylate Cyclase-Activating Polypeptide(PACAP)-like immunoreactivity in the rat retina."Peptides. 21. (2000)
[Publications] S. Shioda: "Pituitary adenylate cyclase activating polypeptide(PACAP)and its receptors in the brain."Acta Anat Nipponica. (in press). (2000)
[Publications] H. Mizushima: "The effect of cardiac arrest on the permeability of the mouse blood-brain and blood-spinal cord barrier to PACAP."Ann NY Acad Sci. (in press. (2000)

1999 Fiscal Year Annual Research Report

PACAP受容体のバソプレシン分泌・合成制御機構

Principal Investigator

塩田 清二 昭和大学, 医学部, 教授 (80102375)

Research Products

[Publications] H. Ozawa: "Delayed neuronal cell death in the rat hippocampus is mediated by the mitogen-activated protein kinase signal transduction pathway."Neurosci Lett. 262. 57-60 (1999)

[Publications] K. Ito: "Bioluminescent enzyme immunoassay for pituitary adenylate cyclase activating polypeptide 38(PACAP38)"Analytica Chimica Acta. 382. 245-251 (1999)

[Publications] H. Funahashi: "The effect of leptin on feeding-regulating neurons in the hypothalamus."Neurosci Lett. 264. 117-120 (1999)

[Publications] S. Muroya: "Glucose-sensitive neurons in the rat arcuate nucleus contain neuropeptide Y."Neurosci Lett. 264. 113-116 (1999)

[Publications] C. J. Zhou: "Ontogenetic expression of PACAP receptor in the rat brain: early in the neural plate at primitive stage."Neuroscience. 93. 375-391 (1999)

[Publications] H. Mizushima: "Effect of cardiac arrest on the permeability of the blood-brain and blood-spinal cord barrier to albumin and tumor necrosis factor-a."Life Sci. 65. 2127-2134 (1999)

[Publications] H. Mizushima: "The effect of cardiac arrest on the permeability of the mouse blood-brain and blood-spinal cord barriers to pituitary adenylate cyclase activating polypeptide(PACAP)."Peptides. 20. 1337-1340 (1999)

[Publications] M. Nakata: "Insulinotropin PACAP potentiates insulin-stimulated glucose uptake in 3T3-L1 cells."Peptides. 20. 943-948 (1999)

[Publications] C. J. Zhou: "Cellular distribution of the splice variants of the receptor for pituitary adenylate cyclase-activating polypeptide(PAC1-R) in the rat brain by in situ RT-PCR."Mol Brain Res. 95. 150-158 (2000)

[Publications] T. Seki: "The distribution and ultrastructural localization of Pituitary Adenylate Cyclase-Activating Polypeptide(PACAP)-like immunoreactivity in the rat retina."Peptides. 21. (2000)

[Publications] S. Shioda: "Pituitary adenylate cyclase activating polypeptide(PACAP)and its receptors in the brain."Acta Anat Nipponica. (in press). (2000)

[Publications] H. Mizushima: "The effect of cardiac arrest on the permeability of the mouse blood-brain and blood-spinal cord barrier to PACAP."Ann NY Acad Sci. (in press. (2000)

塩田清二昭和大学, 医学部, 教授 (80102375)