1999 Fiscal Year Final Research Report Summary
ISCHEMIC TOLERANCE DEPRESSED INIRACELLULAR CALCIUM ELEVATION IN GERBILHIPPOCAMPUS
| Project/Area Number |
10680749
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Jichi Medical School |
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Principal Investigator |
SHIMAZAKI Kuniko Jichi Medical School, Dept of Physiol, Assistant Professor, 医学部, 講師 (40142153)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Bcl-2 / apoptosis / gerbil / hippocampus / brain ischermia / adeno-associated virus vecoor / delayed neuronal death |
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| Research Abstract |
In the gerbil hippocampus, a short (2mim) sublethal ischemic episode preceding lethal (5mim) ischemia is known to increase tolerance against death in CA1 pyramydal neurons. Intracellular calcium elevation level after exposing to the anoxic-aglycemic medium (95%NィイD22ィエD2/5%C0ィイD22ィエD2, glucose-free) in the rhod-2-loaded hippocampal slices was compared between ischemia-tolerant and non-tolerant gerbils. In non-tolerant gerbils, after switching the normal ringer to anoxic-aglycemic medium an abrupt increase in fluorescence in the Ca1area was seen. Thereafter, a steady increase in intracellular calcium took place with the peak fluorescence intensity at nearly 3 times of the base line. In ischemia-tolerant gerbils, however, the fluorescence increase stayed at significantly lower level than non-tolerant gerbils. Tolerance for ischema was known to be acquired from 1 to 7 days after 2 min ischemia but after 14 days no protective effect was observed. Pretreatment with 1 min ischemia was found to be too brief to induce tolerance. In agreement with these data, magnitude of fluorescence elevation in gerbils at 14 days after 2 min ischemia and at 2 days after 1 min ischemia were almost the same as in the non-tolerant gerbils. Our results suggest that conditioning sublethal ischemia induced to tolerance for ischemic neuronal death which is deeply related to acute increase in intracellular calcium.
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[Publications] Shimazaki,K.,Urabe,M.,Miyawaki, T,Masuzawa,T., Kurtzman, G.J., Ozawa, K, And kawai,N.: "Overexpression of Bcl-2 with adeno-associated virus vectors protects hippocampal neurons against ischemic cell death in vivo."Neurosci. Res.. 22, Suppl.. S35 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Shimazaki, K., Nakamura, T., Nakamura, K., Hirata, NI., Oguro, K., Miyawaki, T., Masuzawa, T., Kudo, Y. and Kawai, N: "Ischemic torelance depressed intercellular calcium elevation in gerbil hippocampus."Soc. Neurosci. Abstr.. 24. 807 (1998)
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[Publications] Cho, H., Shimazaki, K., Takauchi, K., Kobayash S Watanabe, K., Oguro, K., Masuzawa,T. and Kawai, N: "Biphasic changes in F3/contactin expression in the gerbil hippocampus after transient ischemia."Exp Brain Res. 122. 227-234 (1998)
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[Publications] Shimazaki, K., Nakamura, T., Nakamura, K., Oguro, K., Masuzawa, I., Kudo, Y. and Kawai, N: "Reduced calcium elevation in hippocampal Ca1 neurons of ischemia-tolerant gerbils."NeuroReport.. 9. 1875-1878 (1998)
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[Publications] Urabe, M., Shimazaki, K, Kume, A., Monahan, J., Kawai, N. and Ozawa, K: "Protection of hippocampal neurons from ischemic cell death by AAV vector expressing Bcl-2 in gerbils."Soc Gene Therapy Abstr.. 2. 51 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Urabe, M., Shimazaki, K., Kume, A., Manahan, J., Kawai, N. and Ozawa, K.: "Adeno-associated virus vector expressing Bcl-2 protects hippocampal neurons from ischemic cell death in vivo."The Japan Society of Gene Therapy the 5th annual meeting abstract. 83. 109 (1999)
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[Publications] Shimazaki, K., Urabe,,M., Monahan, J., Mizui,T.. Kawai, N. and Ozawa, K.: "Bcl-2 protects late stage of delayed neuronal death of hippocampal CA1 cells after ischemia."Neurosci. Abstr.. 25. 1583 (1999)
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