1999 Fiscal Year Final Research Report Summary
Hydrostatic pressure influencing cytokine expression in osteoblast
| Project/Area Number |
10838027
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
リハビリテーション科学
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
ISHIDA Toshihiro Kyoto Prefectural University of Medicine, the medical department, Assistant, 医学部, 助手 (20295653)
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| Co-Investigator(Kenkyū-buntansha) |
KUBO Toshikazu Kyoto Prefectural University of Medicine, the medical department, Associate Professor, 医学部, 助教授 (20178031)
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| Project Period (FY) |
1998 – 1999
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| Keywords | osteoblast / MG63 / hydrostatic pressure / stress / cytokine / IL-8 / HSP70 |
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| Research Abstract |
The present study investigated the influence of hydrostatic pressure (HP) on the cytokine expression by an osteoblast cell line, MG-63. The cells were cultured in dishes until they reach a confluent level, and then exposed to HP of 1, 10, or 50 Ma for 2 h in an apparatus that can add pressure under sterile conditions. Twelve and 24 h after giving the exposure, interleukin (IL)-1α, IL-1β, IL-6, IL-8, tumor necrosis factor (TNF)-α, interferon (IFN)- α and IFN-γ levels in culture supernatants were measured with ELISA method. Total RNA was extracted 4 h after the exposure with the acid guanidinium thiocyanate-phenolchloroform method, and IL-8 mRNA expression was confirmed by Northern blot hybridization using a digoxigenin-labeled probe. The same procedure was repeated for the human fibroblast cell line TIG-3, as the control.
After the exposure to 1, 10 or 50 MPa of HP, IL-1α, IL-1β, IL-6, TNF-α, IFN-α and IFN-γ levels in MG-63 culture were lower than the minimum detectable level (20pg/ml). However, 12 and 24 h after the exposure to 50 MPa, IL-8 levels increased to 3 times the normal levels, and the expression of IL-8 mRNA also increased. In the control TIG-3 cells, levels of all cytokines examined after HP exposure were lower than the minimum detectable level.
These findings indicate that excessive mechanical stress can induce IL-8 production in osteoblasts. Mechanical factor may induced inflammatory reaction in bone tissues that then could result in bone destruction.
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