1999 Fiscal Year Annual Research Report

テトラヒメナのFK506結合蛋白質の研究

Research Project

Project/Area Number	10878123
Research Institution	University of Tsukuba
Principal Investigator	沼田治筑波大学, 生物科学系, 助教授 (50189354)
Keywords	FK506 / FKBP12 / テトラヒメナ / カルシニューリン / 細胞質分裂
Research Abstract	FK506結合蛋白質(FKBP)は免疫抑制剤FK506と結合するとカルシニューリンの機能を阻害し、免疫担当細胞の働きを抑えることが知られている。我々はテトラヒメナにもFKBPが存在することを発見した。これは、この蛋白質が普遍的な生理機能に関与することを示唆している。この普遍的な生理機能を明かにすることが本研究の目的である。 1.テトラヒメナのFKBP遺伝子をクローニングし、そのcDNAの全塩基配列を決定した。推定されたアミノ酸配列からテトラヒメナのFKBPは分子量12kDaでFK506結合部位を保持していた。ホモロジー検索の結果、ショウジョウバエ、ヒト、マウスのFKBP12と約60%の相同性を持ち、類似性は80%近いことが判った。 2.テトラヒメナの同調分裂細胞をFK506で処理したところ細胞質分裂が阻害されることが明らかになった。 3.テトラヒメナFKBP12の機能を明らかにするため:(1)抗-FKBP12抗体を用いたFKBP12の細胞内局在性の決定、(2)FKBP12に結合するカルシニューリンのような蛋白質の同定、などを計画した。その第一段階として大腸菌でリコンビナントFKBP12の大量発現を行い、リコンビナントFKBP12をウサギとモルモットに注射して抗-FKBP12抗体の作製に成功した。

Research Products
(10 results)

All Other

All Publications (10 results)

[Publications] Numata.O.,Kurasawa,H.,and Watanabe.Y.: "Tetrahymena elongation factor-1α binds to Hsp70 family proteins."Zoological Science. 16. 279-284 (1999)
[Publications] Fujiu,K.,and Numata,O.: "Localization of microtubules during macronuclear division in Tetrahymena and possible involvement of macronuclear microtubules in amitotical' chromatin distribution."Cell Structure and Function. 24. 401-404 (1999)
[Publications] Gonda,K.,Nishibori,K.,Ohba,H.,Watanabe,A.,and Numata,O.: "Molecular cloning of the gene for p85 that regulates the initiation of Cytokinesis in Tetrahymena."Biochemical and Biophysical Research Communication. 246. 112-118 (1999)
[Publications] Gonda,K.,Katoh,M.,Hanyu,K.,Watanabe,Y.,and Numata.,O.: "Ca^<2+>/calmodulin and p85 cooperatively regulate an initiation of cytokinesis in Tetrahymena."Journal of Cell Science. 112. 3619-3626 (1999)
[Publications] Wang,S.,Nakashima,S.,Numata,O.,Fujiu,K.,and Nozawa,Y.: "Molecular cloning and cell-cycle-dependent expression of the acetyl-CoA synthetase gene in Tetrahymena cells."Biochem J.. 343. 479-485 (1999)
[Publications] Numata,O.,Kurasawa,H.,Gonda,K.,and Watanabe.Y.: "Tetrahymena elongation factor-1 α is localized with calmodulin in the division furrow."Journal of Biochemistry. 127. 51-56 (2000)
[Publications] Watanabe,A.,Yonemura,I.,Gonda,K.,and Numata.,O.: "Cloning and sequencing of the gene for a Tetrahymena fimbrin-like protein."Journal of Biochemistry. 127. 85-94 (2000)
[Publications] Numata,O.,Gonda,K.,Watanabe,A.,and Kurasawa,Y.: "Cytokinesis in Tetrahymena: determination of devision plane and organization of contractile ring."Microsco.Res.Tech.. (in press). (2000)
[Publications] Numata,O.,Fujiu,K.,and Gonda,K.: "Macronuclear division and cytokinesis in Tetrahymena."Cell Biol.Int.. (in press). (2000)
[Publications] Numata,O.,Hanyu,K.,Takeda,T.,and Watanabe,Y.: "Tetrahymena calcium-binding proteins,TCBP-23 and TCBP25."In Tetrahymena thermophila,ed.by D.J.Asai and J.D.Forney,Methods in Cell Biology,62巻,Academic Press.. 11 (1999)

1999 Fiscal Year Annual Research Report

テトラヒメナのFK506結合蛋白質の研究

Principal Investigator

沼田 治 筑波大学, 生物科学系, 助教授 (50189354)

Research Products

[Publications] Numata.O.,Kurasawa,H.,and Watanabe.Y.: "Tetrahymena elongation factor-1α binds to Hsp70 family proteins."Zoological Science. 16. 279-284 (1999)

[Publications] Fujiu,K.,and Numata,O.: "Localization of microtubules during macronuclear division in Tetrahymena and possible involvement of macronuclear microtubules in amitotical' chromatin distribution."Cell Structure and Function. 24. 401-404 (1999)

[Publications] Gonda,K.,Nishibori,K.,Ohba,H.,Watanabe,A.,and Numata,O.: "Molecular cloning of the gene for p85 that regulates the initiation of Cytokinesis in Tetrahymena."Biochemical and Biophysical Research Communication. 246. 112-118 (1999)

[Publications] Gonda,K.,Katoh,M.,Hanyu,K.,Watanabe,Y.,and Numata.,O.: "Ca^<2+>/calmodulin and p85 cooperatively regulate an initiation of cytokinesis in Tetrahymena."Journal of Cell Science. 112. 3619-3626 (1999)

[Publications] Wang,S.,Nakashima,S.,Numata,O.,Fujiu,K.,and Nozawa,Y.: "Molecular cloning and cell-cycle-dependent expression of the acetyl-CoA synthetase gene in Tetrahymena cells."Biochem J.. 343. 479-485 (1999)

[Publications] Numata,O.,Kurasawa,H.,Gonda,K.,and Watanabe.Y.: "Tetrahymena elongation factor-1 α is localized with calmodulin in the division furrow."Journal of Biochemistry. 127. 51-56 (2000)

[Publications] Watanabe,A.,Yonemura,I.,Gonda,K.,and Numata.,O.: "Cloning and sequencing of the gene for a Tetrahymena fimbrin-like protein."Journal of Biochemistry. 127. 85-94 (2000)

[Publications] Numata,O.,Gonda,K.,Watanabe,A.,and Kurasawa,Y.: "Cytokinesis in Tetrahymena: determination of devision plane and organization of contractile ring."Microsco.Res.Tech.. (in press). (2000)

[Publications] Numata,O.,Fujiu,K.,and Gonda,K.: "Macronuclear division and cytokinesis in Tetrahymena."Cell Biol.Int.. (in press). (2000)

[Publications] Numata,O.,Hanyu,K.,Takeda,T.,and Watanabe,Y.: "Tetrahymena calcium-binding proteins,TCBP-23 and TCBP25."In Tetrahymena thermophila,ed.by D.J.Asai and J.D.Forney,Methods in Cell Biology,62巻,Academic Press.. 11 (1999)

沼田治筑波大学, 生物科学系, 助教授 (50189354)