1999 Fiscal Year Annual Research Report

p300/CBPを介するコアクチベーターネットワークと胚性幹細胞の分化誘導

Research Project

Project/Area Number	11154230
Research Institution	The Institute of Physical and Chemical Research
Principal Investigator	横山一成理化学研究所, 細胞材料開発室, 副主任研究員 (80182707)
Keywords	レチノイン酸 / p300 / CBP / F9細胞 / クロマチンリモデリング / RAR / RXR / アンチセンス / シグナルカスケード / リボザイム
Research Abstract	胚性幹細胞のレチノイン酸による内胚葉系細胞への分化誘導に必要なc-jun遺伝子のトランス活性化に必要な転写因子(DRF : Differentiation Regulatory Factor)の構成成分をYeast2 Hybird法によりATF-2,JDP-2(Jun dimerization protein-2),p300と同定した。JDP-2はATF-2のみならず、c-junとも分子会合するbZip転写因子である。現在、これらの両者の結合ドメインのマッピングを行っている。p300,CBPリボザイムクローンの解析によりc-junのトランス活性化にはp300ネットワークが必要であることを明らかにした。この結合はアンチセンスオリゴマーを用いた独立の解析からも指示された。p300とCBPのRA依存性の終末分化の過程においてp300はコビキチン化され、CBPに比べるとより分解されやすい。更にATF-2とp300/CBPのHAT活性の解析より、ATF-2はp300/CBPのHAT活性を促進し、ヒストンのH2bとH4をin vivo,in vitroでアセチル化する。現在RAX,RXRのどのレセプターがp300とCBPのカスケードネットワークと関連しているかを解析中である。

Research Products

(15 results)

All Other

All Publications (15 results)

[Publications] Nishitani, et al.: "Recruitment of the retinoblastoma protein to c-Jun enhances transcription activity mediated through the AP-1 binding site"J. Biol. Chem.. 274. 5454-5461 (1999)
[Publications] Song, J., et al.: "Structural organization and expression of the mouse gene for Pur-1, a highly conserved homolog of the human MAZ gene"Eur. J. Biochem.. 259. 676-683 (1999)
[Publications] Zhao, X. X., et al.: "Mannosylerythritol lipid is a potent inducer of apoptosis and differentiation of mouse melanoma cells in culture"Cancer Research. 59. 482-486 (1999)
[Publications] Yuan, Z-M., et al.: "Role for p300 in stabilization of p53 in the response to DNA damage"J. Biol. Chem.. 274. 1883-1886 (1999)
[Publications] Tsutsui, H., et al.: "The DNA-binding and transcriptional activities of MAZ, a Myc-associated zinc finger protein, are regulated by casein kinase II"Biochem. Biophys. Res. Commun.. 226. 198-205 (1999)
[Publications] Ugai, H., et al.: "The co-activators p300 and CBP have different functions during the differentiationof F9 cells. (Review)"J. Mol. Medicine. 77. 481-494 (1999)
[Publications] Song, j., et al.: "Removal of ethidium bromide and cesium chloride from plasmid DNA by ethanol precipitation"BioTechnique. 26. 1056-1060 (1999)
[Publications] Song, j., et al.: "Direct lysis method for the rapid perparation of plasmid DNA"Anal. Biochem.. 271. 89-91 (1999)
[Publications] Koga, C., et al.: "Characterization of a novel member of the FGF family, XFGF-20, in Xenopus laevis"Biochem. Biophys. Res. Commun.. 261. 756-765 (1999)
[Publications] Kawasaki, H., et al.: "Functional analysis of the transcriptional coactivators p300 and CBP using ribozyme"Nucleic Acids Symposium Series. 42. 263-264 (1999)
[Publications] Iwao, K., et al.: "Ubiqutination of transcriptional coactivator p300 during retinoic acid induced differentiation"Nucleic Acids Symposium Series. 42. 208-209 (1999)
[Publications] 川崎弘明 et al.: "「転写因子研究」「転写の司令塔p300/CBPのコアクチベータ機能」"実験医学. 219-228 (1999)
[Publications] 横山和尚: "「遺伝子治療開発研究ハンドブック」「アンチセンス法による遺伝子情報ノックアウト戦略法」"日本遺伝子治療学会. 584-595 (1999)
[Publications] Kawasaki, H., et al.: "Ribozyme Technology and Applications"Academic Press. 1-26 (2000)
[Publications] 横山和尚: "胚性幹腫瘍細胞F9を用いた発生、分化、再生研究「Science & Techno News」"つくば支援センター. 9-13 (1999)

1999 Fiscal Year Annual Research Report

p300/CBPを介するコアクチベーターネットワークと胚性幹細胞の分化誘導

Principal Investigator

横山 一成 理化学研究所, 細胞材料開発室, 副主任研究員 (80182707)

Research Products

[Publications] Nishitani, et al.: "Recruitment of the retinoblastoma protein to c-Jun enhances transcription activity mediated through the AP-1 binding site"J. Biol. Chem.. 274. 5454-5461 (1999)

[Publications] Song, J., et al.: "Structural organization and expression of the mouse gene for Pur-1, a highly conserved homolog of the human MAZ gene"Eur. J. Biochem.. 259. 676-683 (1999)

[Publications] Zhao, X. X., et al.: "Mannosylerythritol lipid is a potent inducer of apoptosis and differentiation of mouse melanoma cells in culture"Cancer Research. 59. 482-486 (1999)

[Publications] Yuan, Z-M., et al.: "Role for p300 in stabilization of p53 in the response to DNA damage"J. Biol. Chem.. 274. 1883-1886 (1999)

[Publications] Tsutsui, H., et al.: "The DNA-binding and transcriptional activities of MAZ, a Myc-associated zinc finger protein, are regulated by casein kinase II"Biochem. Biophys. Res. Commun.. 226. 198-205 (1999)

[Publications] Ugai, H., et al.: "The co-activators p300 and CBP have different functions during the differentiationof F9 cells. (Review)"J. Mol. Medicine. 77. 481-494 (1999)

[Publications] Song, j., et al.: "Removal of ethidium bromide and cesium chloride from plasmid DNA by ethanol precipitation"BioTechnique. 26. 1056-1060 (1999)

[Publications] Song, j., et al.: "Direct lysis method for the rapid perparation of plasmid DNA"Anal. Biochem.. 271. 89-91 (1999)

[Publications] Koga, C., et al.: "Characterization of a novel member of the FGF family, XFGF-20, in Xenopus laevis"Biochem. Biophys. Res. Commun.. 261. 756-765 (1999)

[Publications] Kawasaki, H., et al.: "Functional analysis of the transcriptional coactivators p300 and CBP using ribozyme"Nucleic Acids Symposium Series. 42. 263-264 (1999)

[Publications] Iwao, K., et al.: "Ubiqutination of transcriptional coactivator p300 during retinoic acid induced differentiation"Nucleic Acids Symposium Series. 42. 208-209 (1999)

[Publications] 川崎弘明 et al.: "「転写因子研究」「転写の司令塔p300/CBPのコアクチベータ機能」"実験医学. 219-228 (1999)

[Publications] 横山和尚: "「遺伝子治療開発研究ハンドブック」「アンチセンス法による遺伝子情報ノックアウト戦略法」"日本遺伝子治療学会. 584-595 (1999)

[Publications] Kawasaki, H., et al.: "Ribozyme Technology and Applications"Academic Press. 1-26 (2000)

[Publications] 横山和尚: "胚性幹腫瘍細胞F9を用いた発生、分化、再生研究「Science & Techno News」"つくば支援センター. 9-13 (1999)

横山一成理化学研究所, 細胞材料開発室, 副主任研究員 (80182707)