1999 Fiscal Year Annual Research Report

がんの生物学的特性に関する研究の総括

Research Project

Project/Area Number	11179101
Research Category	Grant-in-Aid for Scientific Research on Priority Areas (A)
Research Institution	Osaka University
Principal Investigator	高井義美大阪大学, 医学系研究科, 教授 (60093514)
Co-Investigator(Kenkyū-buntansha)	清木元治東京大学, 医科学研究所, 教授 (10154634) 秋山徹東京大学, 分子細胞生物学研究所, 教授 (70150745) 平井久丸東京大学, 医学部・附属病院, 助教授 (90181130) 谷口維紹東京大学, 医学系研究所, 教授 (50133616) 渋谷正史東京大学, 医科学研究所, 教授 (10107427)
Keywords	がん / 増殖因子 / シグナル伝達 / 分化 / 細胞死 / 細胞周期 / 浸潤・転移 / 細胞外マトリックス
Research Abstract	本領域は「がん研究の総合的推進に関する研究」の一環として、がんの生物学的研究を担うものである。すなわち、がんの生物学的特性を明らかにすることによって、がんの予防、診断、治療に貢献することを目的とする。本年度の総括班における成果を以下に列記する。 (1)がんの生物学的研究の分野において未解決の重要な問題を解明するために、総括班は以下の5項目についての研究を計画した。A01:がん細胞の増殖応答の異常の研究、A02:がんの形成過程における細胞の分化と死の研究、A03:がん細胞の制御異常とがん形質の研究、A04:がん細胞の浸潤と転移の研究、A05:組織形成異常とがんとの関連の研究。 (2)領域代表者である高井義美をA01の班長とし、以下、A02平井久丸、A03秋山徹、A04清木元治、A05渋谷正史とした。また、本研究分野で顕著な業績を持つ研究者5名(谷口維紹、宮島篤、月田承一郎、西田栄介、広橋説雄)を総括班員に加え、主として研究計画実施の方針に対する助言および各研究項目長とともに研究成果の評価にあたることとした。 (3)研究を推進するために、重要かつ緊急性のあるテーマと優れた研究者を計画的に支援する目的で、各項目ごとで、総計27の計画研究を設定し、これを担当する研究代表者と分担者の選定と研究内容の評価を行った。また、公募により自由な発想に基づく研究を募集し、この研究内容を評価した。 (4)総括班会議および分野長会議を開催しており、上記重要課題につき検討の上、方針を決定し領域の円滑な運営を進めた。このように、私共は、本年度の予定をほぼ達成することができた。

Research Products

(20 results)

All Other

All Publications (20 results)

[Publications] Umikawa, M.: "Association of frabin with the actin cytoskeleton is essential for microspike formation through activation of Cdc42 small G protein"J. Biol. Chem.. 274・36. 25197-25200 (1999)
[Publications] Ikeda, W.: "Afadin : A key molecule essential for structural organization of cell-cell junctions of polarised epithelia during embryogenesis"J. Cell. Biol.. 146・5. 1117-1132 (1999)
[Publications] Shimizu, K.: "Mouse jagged1 physically interacts with notch2 and other notch receptors : Assessment by quantitative methods"J. Biol. Chem.. 274・46. 32961-32969 (1999)
[Publications] Nakamoto, T.: "CIZ : a zinc finger protein that interacts with p130cas and activates the expression of matrix metalloproteinases"Mol. Cell. Biol.. 20・5. 1649-1658 (2000)
[Publications] Hamada, F.: "Negative regulation of Wingless signaling by D-axin, a Drosophila homolog of axin"Science. 283・5408. 1739-1742 (1999)
[Publications] Ishidate, T.: "The APC-hDLG complex negatively regulates cell cycle progression from the G0/G1 to S phase"Oncogene. 19・3. 365-372 (2000)
[Publications] Itoh, Y.: "Membrane type 4 matrix metalloproteinase (MT4-MMP, MMP-17) is a qlycosylphosphatidylinositol-anchored proteinase"J. Biol. Chem.. 274・48. 34260-34266 (1999)
[Publications] Kajita, M.: "Human membrane type-4 matrix metalloproteinase (MT4-MMP) is encoded by a novel major transcript : isolation of complementary DNA clones for human and mouse mt4-mmp transcripts [In Process Citation]"FEBS Lett.. 457・3. 353-356 (1999)
[Publications] Shibuya, M.: "Structure and function of vascular endothelial growth factor receptor-1 and -2"Curr. Top. Microbiol. Immunol.. 237. 59-83 (1999)
[Publications] Takahashi, T.: "VEGF activates protein kinase C-dependent, but Ras-independent Raf-MEK-MAP kinase pathway for DNA synthesis in primary endothelial cells"Oncogene. 18・13. 2221-2230 (1999)
[Publications] Takaoka, A.: "Protein tyrosine kinase Pyk2 mediates the Jak-dependent activation of MAPK and Stat1 in the IFN-γ, but not IFN-α, signaling"EMBO J.. 18・9. 2480-2488 (1999)
[Publications] Nozawa, H.: "Loss of transcription factor IRF-1 affects tumor susceptibility in mice carrying the Ha-ras transgene or nullizygosity for p53"Genes Dev.. 13・10. 1240-1245 (1999)
[Publications] Kamiya, A.: "Fetal liver development requires a paracrine action of oncostatin M through the gp130 signal transducer"EMBO J.. 18・8. 2127-2136 (1999)
[Publications] Hara, T.: "Identification of podocalyxin-like protein 1 as a novel cell surface marker for hemangioblasts in the murine aorta-gonad-mesonephros region"Immunity. 11・5. 567-578 (1999)
[Publications] Morita, K.: "Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands"Proc. Natl. Acad. Sci. USA. 96・2. 511-516 (1999)
[Publications] Morita, K.: "Claudin-11/OSP-based tight junctions in myelin sheaths in brain and Sertoli cells in testis"J. Cell Biol.. 145・3. 579-588 (1999)
[Publications] Akashi, M.: "Involvement of the MAP kinase cascade in resetting of the mammalian circadian clock"Genes Dev.. (in press). (2000)
[Publications] Tanoue, T.: "A conserved docking motif in MAP kinases common to substrates, activators and regulators"Nat. Cell Biol.. 2・2. 110-116 (2000)
[Publications] Ono, Y.: "Clinicopathologic significance of laminin-5 γ2 chain expression in squamous cell carcinoma of the tongue : immunohistochemical analysis of 67 lesions"Cancer. 85・11. 2315-2321 (1999)
[Publications] Genda, T.: "Cell motility mediated by Rho and Rho-associated protein kinase plays a critical role in intrahepatic metastasis of human hepatocellular carcinoma"Hepatology. 30・4. 1027-1036 (1999)

1999 Fiscal Year Annual Research Report

がんの生物学的特性に関する研究の総括

Principal Investigator

高井 義美 大阪大学, 医学系研究科, 教授 (60093514)

Research Products

[Publications] Umikawa, M.: "Association of frabin with the actin cytoskeleton is essential for microspike formation through activation of Cdc42 small G protein"J. Biol. Chem.. 274・36. 25197-25200 (1999)

[Publications] Ikeda, W.: "Afadin : A key molecule essential for structural organization of cell-cell junctions of polarised epithelia during embryogenesis"J. Cell. Biol.. 146・5. 1117-1132 (1999)

[Publications] Shimizu, K.: "Mouse jagged1 physically interacts with notch2 and other notch receptors : Assessment by quantitative methods"J. Biol. Chem.. 274・46. 32961-32969 (1999)

[Publications] Nakamoto, T.: "CIZ : a zinc finger protein that interacts with p130cas and activates the expression of matrix metalloproteinases"Mol. Cell. Biol.. 20・5. 1649-1658 (2000)

[Publications] Hamada, F.: "Negative regulation of Wingless signaling by D-axin, a Drosophila homolog of axin"Science. 283・5408. 1739-1742 (1999)

[Publications] Ishidate, T.: "The APC-hDLG complex negatively regulates cell cycle progression from the G0/G1 to S phase"Oncogene. 19・3. 365-372 (2000)

[Publications] Itoh, Y.: "Membrane type 4 matrix metalloproteinase (MT4-MMP, MMP-17) is a qlycosylphosphatidylinositol-anchored proteinase"J. Biol. Chem.. 274・48. 34260-34266 (1999)

[Publications] Kajita, M.: "Human membrane type-4 matrix metalloproteinase (MT4-MMP) is encoded by a novel major transcript : isolation of complementary DNA clones for human and mouse mt4-mmp transcripts [In Process Citation]"FEBS Lett.. 457・3. 353-356 (1999)

[Publications] Shibuya, M.: "Structure and function of vascular endothelial growth factor receptor-1 and -2"Curr. Top. Microbiol. Immunol.. 237. 59-83 (1999)

[Publications] Takahashi, T.: "VEGF activates protein kinase C-dependent, but Ras-independent Raf-MEK-MAP kinase pathway for DNA synthesis in primary endothelial cells"Oncogene. 18・13. 2221-2230 (1999)

[Publications] Takaoka, A.: "Protein tyrosine kinase Pyk2 mediates the Jak-dependent activation of MAPK and Stat1 in the IFN-γ, but not IFN-α, signaling"EMBO J.. 18・9. 2480-2488 (1999)

[Publications] Nozawa, H.: "Loss of transcription factor IRF-1 affects tumor susceptibility in mice carrying the Ha-ras transgene or nullizygosity for p53"Genes Dev.. 13・10. 1240-1245 (1999)

[Publications] Kamiya, A.: "Fetal liver development requires a paracrine action of oncostatin M through the gp130 signal transducer"EMBO J.. 18・8. 2127-2136 (1999)

[Publications] Hara, T.: "Identification of podocalyxin-like protein 1 as a novel cell surface marker for hemangioblasts in the murine aorta-gonad-mesonephros region"Immunity. 11・5. 567-578 (1999)

[Publications] Morita, K.: "Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands"Proc. Natl. Acad. Sci. USA. 96・2. 511-516 (1999)

[Publications] Morita, K.: "Claudin-11/OSP-based tight junctions in myelin sheaths in brain and Sertoli cells in testis"J. Cell Biol.. 145・3. 579-588 (1999)

[Publications] Akashi, M.: "Involvement of the MAP kinase cascade in resetting of the mammalian circadian clock"Genes Dev.. (in press). (2000)

[Publications] Tanoue, T.: "A conserved docking motif in MAP kinases common to substrates, activators and regulators"Nat. Cell Biol.. 2・2. 110-116 (2000)

[Publications] Ono, Y.: "Clinicopathologic significance of laminin-5 γ2 chain expression in squamous cell carcinoma of the tongue : immunohistochemical analysis of 67 lesions"Cancer. 85・11. 2315-2321 (1999)

[Publications] Genda, T.: "Cell motility mediated by Rho and Rho-associated protein kinase plays a critical role in intrahepatic metastasis of human hepatocellular carcinoma"Hepatology. 30・4. 1027-1036 (1999)

高井義美大阪大学, 医学系研究科, 教授 (60093514)