2001 Fiscal Year Annual Research Report

がんの生物学的特性に関する研究の総括

Research Project

Project/Area Number	11179101
Research Institution	Osaka University
Principal Investigator	高井義美大阪大学, 医学系研究科, 教授 (60093514)
Co-Investigator(Kenkyū-buntansha)	清木元治東京大学, 医科学研究所, 教授 (10154634) 秋山徹東京大学, 分子細胞生物学研究所, 教授 (70150745) 平井久丸東京大学, 医学部・附属病院, 助教授 (90181130) 谷口継紹東京大学, 医学系研究科, 教授 (50133616) 渋谷正史東京大学, 医科学研究所, 教授 (10107427)
Keywords	がん / 増殖因子 / シグナル伝達 / 分化 / 細胞死 / 細胞周期 / 浸潤・転移 / 細胞外マトリックス
Research Abstract	本領域は「がん研究の総合的推進に関する研究」の一環として、がんの生物学的研究を担うものである。すなわち、がんの生物学的特性を明らかにすることによって、がんの予防、診断治療に貢献することを目的とする。本年度の総括班における成果を以下に列記する。 1.がんの生物学的研究の分野において未解決の重要な問題を解明するために、総括班は以下の5項目について研究を計画した。A01:がん細胞の増殖応答の異常の研究、A02:がんの形成過程における細胞の分化と死の研究、A03:がん細胞の制御異常とがん形質の研究、A04:がん細胞の浸潤と転移の研究、A05:組織形成異常とがんとの関連の研究。 2.領域代表者である高井義美をA01の班長とし、以下、A02平井久丸、A03秋山徹、A04清木元治、A05渋谷正史とした。また、本研究分野で顕著な業績を持つ研究者5名(谷口維紹、宮島篤、月田承一郎、西田栄介、広橋説雄)を総括班に加え、主として研究計画実施の方針に対する助言および各研究項目長とともに研究成果の評価にあたることとした。 3.研究を推進するために、重要かつ緊急性のあるテーマと優れた研究者を計画的に支援する目的で、各項日ごとで、総計41の研究計画を設定し、これに担当する研究代表者と分担者の選定と研究内容の評価を行った。また、公募により自由な発想に基づく研究を募集し、この研究内容を評価した。 4.総括班会議および分野長会議を開催しており、上記重要課題につき検討上、方針を決定し領域の円滑な運営を進めた。このように、私共は、本年度の予定をほぼ達成することができた。

Research Products

(20 results)

All Other

All Publications (20 results)

[Publications] Shinohara, M.: "Roles of cell-cell adhesion-dependent tyrosine phosphorylation of Gab-1"J. Biol. Chem.. 276・22. 18941-18946 (2001)
[Publications] Yokoyama, S.: "α-Catenin-independent recruitment of ZO-1 to nectin-based cell-cell adhesion sites through afadin"Mol. Biol. Cell. 12・6. 1595-1609 (2001)
[Publications] Shimizu, K.: "Integrity of intracellular domain of Notch ligand is indispensable for cleavage required for release of the Notch2 intracellular domain"EMBO J.. 21・3. 294-302 (2002)
[Publications] Shimizu, K.: "Manic fringe and lunatic fringe modify different sites of the Notch2 extracellular region, resulting in different signaling modulation"J. Biol. Chem.. 276・28. 25753-25758 (2001)
[Publications] Jimbo, T.: "Link between the Tumor Suppressor APC and the Kinesin Superfamily of Proteins"Nature Cell Biol.. (in press). (2002)
[Publications] Kohu, K.: "The SH3, HOOK and Guanylate Kinase-like Domains of hDLG are Important for its Cytoplasmic Localization"Genes Cells. (in press). (2002)
[Publications] Itoh, Y.: "Homophilic complex formation of MT1-MMP facilitates proMMP-2 activation on the cell surface and promotes tumor cell invasion"EMBO J.. 20・17. 4782-4793 (2001)
[Publications] Kajita, M.: "Membrane-type 1 matrix metalloproteinase cleaves CD44 and promotes cell migration"J. Cell. Biol.. 153・5. 893-904 (2001)
[Publications] Takahashi, T.: "A single autophosphorylation site on KDR/Flk-1 is essential for VEGF-A-dependent activation of PLC-γ and DNA synthesis in vascular endothelial cells"EMBO J.. 20・11. 2768-2778 (2001)
[Publications] Hiratsuka, S.: "Involvement of Flt-1 tyrosine kinase (Vascular endothelial growth factor receptor-1) in pathological angiogenesis"Cancer Res.. 61・3. 1207-1213 (2001)
[Publications] Sato, K.: "Antiviral response by natural killer cells through TRAIL gene induction by IFN-α/β"Eur. J. Immunol.. 31・11. 3138-3146 (2001)
[Publications] Hata, N.: "Constitutive IFN-α/β signal for efficient IFN-α/β gene induction by virus"Biochem. Biophys. Res. Commun.. 285・2. 518-515 (2001)
[Publications] Matsui, T.: "K-Ras mediates cytokine-induced formations of E-cadherin-based adherens junctions during liver development"EMBO J.. 21・5. 1021-1030 (2002)
[Publications] Takeuchi, M.: "Cultivation of AGM-derived hematopoietic stem cells in the fetal liver microenvironment amplifies long-term repopulating activity and enhances engraftment to the bone marrow"Blood. 99・4. 1190-1196 (2002)
[Publications] Itoh, M.: "Junctional adhesion molecule (JAM) binds to PAR-3 : a possible mechanism for the recruitment of PAR-3 to tight junctions"J. Cell Biol.. 154・3. 491-497 (2001)
[Publications] Furuse, M.: "Conversion of zonulae occludentes from tight to leaky strand type by introducing claudin-2 into MDCK I cells"J. Cell Biol.. 153・2. 263-272 (2001)
[Publications] Toyoshima-Morimoto, F.: "Plk1 promotes nuclear translocation of human Cdc25C during prophase"EMBO Reports. (in press). (2002)
[Publications] Yamanaka, H.: "JNK functions in the non-canonical Wnt pathway to regulate convergent extension movements in vertebrates"EMBO Reports. 3・1. 69-75 (2002)
[Publications] Ino, Y.: "Dysadherin, a cancer-associated cell membrane glycoprotein, down-regulates E-cadherin and promotes metastasis"Proc. Natl. Acad. Sci. USA. 99・1. 365-370 (2002)
[Publications] Naishiro, Y.: "Restoration of epithelial cell polarity in a colorectal cancer cell line by suppression of β-catenin/T-cell factor 4-mediated gene transactivation"Cancer Res.. 61・6. 2751-2758 (2001)

2001 Fiscal Year Annual Research Report

がんの生物学的特性に関する研究の総括

Principal Investigator

高井 義美 大阪大学, 医学系研究科, 教授 (60093514)

Research Products

[Publications] Shinohara, M.: "Roles of cell-cell adhesion-dependent tyrosine phosphorylation of Gab-1"J. Biol. Chem.. 276・22. 18941-18946 (2001)

[Publications] Yokoyama, S.: "α-Catenin-independent recruitment of ZO-1 to nectin-based cell-cell adhesion sites through afadin"Mol. Biol. Cell. 12・6. 1595-1609 (2001)

[Publications] Shimizu, K.: "Integrity of intracellular domain of Notch ligand is indispensable for cleavage required for release of the Notch2 intracellular domain"EMBO J.. 21・3. 294-302 (2002)

[Publications] Shimizu, K.: "Manic fringe and lunatic fringe modify different sites of the Notch2 extracellular region, resulting in different signaling modulation"J. Biol. Chem.. 276・28. 25753-25758 (2001)

[Publications] Jimbo, T.: "Link between the Tumor Suppressor APC and the Kinesin Superfamily of Proteins"Nature Cell Biol.. (in press). (2002)

[Publications] Kohu, K.: "The SH3, HOOK and Guanylate Kinase-like Domains of hDLG are Important for its Cytoplasmic Localization"Genes Cells. (in press). (2002)

[Publications] Itoh, Y.: "Homophilic complex formation of MT1-MMP facilitates proMMP-2 activation on the cell surface and promotes tumor cell invasion"EMBO J.. 20・17. 4782-4793 (2001)

[Publications] Kajita, M.: "Membrane-type 1 matrix metalloproteinase cleaves CD44 and promotes cell migration"J. Cell. Biol.. 153・5. 893-904 (2001)

[Publications] Takahashi, T.: "A single autophosphorylation site on KDR/Flk-1 is essential for VEGF-A-dependent activation of PLC-γ and DNA synthesis in vascular endothelial cells"EMBO J.. 20・11. 2768-2778 (2001)

[Publications] Hiratsuka, S.: "Involvement of Flt-1 tyrosine kinase (Vascular endothelial growth factor receptor-1) in pathological angiogenesis"Cancer Res.. 61・3. 1207-1213 (2001)

[Publications] Sato, K.: "Antiviral response by natural killer cells through TRAIL gene induction by IFN-α/β"Eur. J. Immunol.. 31・11. 3138-3146 (2001)

[Publications] Hata, N.: "Constitutive IFN-α/β signal for efficient IFN-α/β gene induction by virus"Biochem. Biophys. Res. Commun.. 285・2. 518-515 (2001)

[Publications] Matsui, T.: "K-Ras mediates cytokine-induced formations of E-cadherin-based adherens junctions during liver development"EMBO J.. 21・5. 1021-1030 (2002)

[Publications] Takeuchi, M.: "Cultivation of AGM-derived hematopoietic stem cells in the fetal liver microenvironment amplifies long-term repopulating activity and enhances engraftment to the bone marrow"Blood. 99・4. 1190-1196 (2002)

[Publications] Itoh, M.: "Junctional adhesion molecule (JAM) binds to PAR-3 : a possible mechanism for the recruitment of PAR-3 to tight junctions"J. Cell Biol.. 154・3. 491-497 (2001)

[Publications] Furuse, M.: "Conversion of zonulae occludentes from tight to leaky strand type by introducing claudin-2 into MDCK I cells"J. Cell Biol.. 153・2. 263-272 (2001)

[Publications] Toyoshima-Morimoto, F.: "Plk1 promotes nuclear translocation of human Cdc25C during prophase"EMBO Reports. (in press). (2002)

[Publications] Yamanaka, H.: "JNK functions in the non-canonical Wnt pathway to regulate convergent extension movements in vertebrates"EMBO Reports. 3・1. 69-75 (2002)

[Publications] Ino, Y.: "Dysadherin, a cancer-associated cell membrane glycoprotein, down-regulates E-cadherin and promotes metastasis"Proc. Natl. Acad. Sci. USA. 99・1. 365-370 (2002)

[Publications] Naishiro, Y.: "Restoration of epithelial cell polarity in a colorectal cancer cell line by suppression of β-catenin/T-cell factor 4-mediated gene transactivation"Cancer Res.. 61・6. 2751-2758 (2001)

高井義美大阪大学, 医学系研究科, 教授 (60093514)