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2002 Fiscal Year Final Research Report Summary

Substrate binding mechanism of P (3HB) depolymerase

Research Project

Project/Area Number 11217202
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionGunma University

Principal Investigator

KASOKA Ken-ichi  Gunma University, Dept of Engineering, Assistant Professor, 工学部, 助手 (60301751)

Project Period (FY) 1999 – 2002
KeywordsP (3HB) / P (3HB) depolymerase / SBD / cDNA / PESu / PESu / SBD
Research Abstract

A P (3HB)-degrading bacterium Marinobacter sp. was isolated from deep sea. A P (3HB) depolymerase produced by the isolate was purified from the culture supernatant and characterized in detail. Further, a gene encoding the enzyme was cloned in E. coli. Genetic analysis revealed that the enzyme is composed of three functional domains, that is, catalytic, linker, and substrate-binding domains. Fungal P (3HB) depolymerase was purified from the supernatant of Penicillium funiculosom. A cDNA encoding the enzyme was cloned. Genetic analysis revealed that the enzyme lacks the substrate-binding domain (SBD) in contrast to bacterial enzymes. Due to the deletion, the fungal enzyme had less binding ability than those of bacterial enzymes. Homology search of the depolymerase genes showed that fungal enzyme gene may be incorporated from the degrading bacterial strain by the horizontal gene transfer. Mesophilic poly (ethylene succinate) (PESu)-degrading strains were isolated from natural environments, and identified. It was found that the degrading species belong to Gram-positive bacterium and fungus.

  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] K.KASUYA: "Cloning, Expression and Characterization of a Poly(3-hydroxybutyrate) Depolymerase from Marinobacter sp. NK-1"Int.J.Biol.Macromol.. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] W.-C.HISEH: "Crystallization and Evaluation of Physical Properties of Microbial Poly(3-hydroxybutvrate-co-4-hydroxybutyrate)"Sen-i Gakkaishi. 58. 428-431 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.WANG: "Enzymatic hydrolysis of bacterial poly(3-hydroxybutyrate-co-3-hydroxypropionate)s by poly(3-hydroxyalkanoate) depolymerase from Acidovorax sp. TP4"Biomacromolecules. 3. 828-834 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.WANG: "Enzymatic hydrolysis of chemosynthesized atactic poly(3-hydroxybutyrate) by poly(3-hydroxyalkanoate) depolymerase from Acidovorax sp. TP4 and Ralstonia pickettii T1"Biomacromolecules. 3. 894-898 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N.YOSHIE: "Change of surface structure of poly(3-hydroxybutyrate) film upon enzymatic hydrolysis by PHB depolymerase"Biomacromolecules. 3. 1320-1326 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.MITOMO: "Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) Produced by Comamonas acidvorans"Polymer. 42. 3455-3461 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.HE: "Enzymatic biodegradation of synthetic atactic poly(R, S-3-hydroxybutyrate) enhanced by an amorphous nonbiodegradable polymer"Polym.Degrad.Stab.. 73. 193-199 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.MIYAZAKI: "Properties of a Poly(3-hydroxybtyrate) Depolymerase from Penicillium funiculosum"J.Polym.Environ. 8. 175-182 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.HIRAISHI: "Function of the Catalytic Domain of Poly(3-hydroxybu tyrirate) Depolymerase from Pseudomonas stutzeri"Biomacromolecules. 1. 320-324 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.HE: "Enzymatic Biodegradation of Chemosynthetic Atactic P(3HB) Enhanced by an Amorphous Nonbiodegradable Polymer : Blend of Astactic P(3HB) with PMMA"Macromol.Rapid Commun.. 21. 1277-1281 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.KASUYA: "Identification of a Marine Benthic P(3HB)-degrading Bacterium Isolate and Characterization of Its P(3HB) Depolymerase"Biomacromolecules. 1. 194-201 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.SUZUKI: "Enzyme-catalyzed ring-opening polymerization of β-butyrolactone using PHB depolymerase"Chem.Lett.. 318-319 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N.YOSHIE: "Effect of low molecular weight additives on enzymatic degradation of poly(3-hydroxybutyrate)"Polymer. 41. 3227-3234 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.OHURA: "Cloning and Characterization of the Polyhydroxybutyrate Depolymerase Gene of Pseudomonas stutzeri and Analysis of the Function of Substrate-binding Domains"Appl.Environ.Microbiol.. 65. 189-197 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N.YOSHIE: "Effect of monomer composition and composition distribution on enzymatic degradation of poly (3-hydroxybutyrate-co-3-hydroxyvalerate)"Macromol.Chem.Phys.. 200. 3227-3234 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.CAOs: "Solid structure and biodegradation of the compositionally fractionated poly(3-hydroxybutyric acid- co-3-hydroxypropionic acid)s"Polymer. 40. 6821-6830 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.OHURA: "Biodegradation of Poly(3-hydroxyalkanoic acids) Fibers and Isolation of Poly(3-hydroxybutyric acid)-degrading Microorganisms under Aquatic Environments"Polym.Degrad.Stab.. 63. 23-29 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.TABATA: "Poly(aspartic acid) Degradation by a Sphingomonas sp. Isolated from Freshwater"Appl.Environ.Microbiol.. 65. 4268-4270 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 粕谷健一: "グリーンプラスチック最新技術"シーエムシー出版社(井上義夫編). 11 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Tabata, K.Kasuya, H.Abe, K.Masuda, Y.Doi: "Poly (aspartic acid) Degradation by a Sphingomonas sp. Isolated from Freshwater"Appl. Environ. Microbiol.. 65. 4268-4270 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Kasuya, T.Ohura, K.Masuda, Y.Doi: "Substrate and Binding Specificities of Bacterial Polyhydroxybutyrate Depolymerases"Int. J.Biol. Macromol.. 24. 329-336 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Ohura, Y.Aoyagi, K.Takagi, Y.Yoshida, K.Kasuya, Y.Doi: "Biodegradation of Poly (3-hydroxyalkanoic acids) Fibers and Isolation of Poly (3-hydroxybutyric acid)-degrading Microorganisms under Aquatic Environments"Polym. Degrad. Stab.. 63. 23-29 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Cao, Y.Arai, N.Yoshie, K.Kasuya, Y.Doi, Y.Inoue: "Solid structure and biodegradation of the compositionally fractionated poly (3-hydroxybutyric acid-co-3-hydroxypropionic acid) s"Polymer. 40. 6821-6830 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Yoshie, M.Fujiwara, K.Kasuya, H.Abe, Y.Doi, Y.Inoue: "Effect of monomer composition and composition distribution on enzymatic degradation of poly (3-hydroxybutyrate-co-3-hydroxyvalerate)"Macromol. Chem. Phys.. 200. 977-982 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Ohura, K.Kasuya, Y.Doi: "Cloning and Characterization of the Polyhydroxybutyrate Depolymerase Gene of Pseudomonas stutzeri and Analysis of the Function of Substrate-binding Domains"Appl. Environ. Microbiol.. 65. 189-197 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Yoshie, K.Nakasato, M.Fujiwara, K.Kasuya, H.Abe, Y.Doi, Y.Inoue: "Effect of low molecular weight additives on enzymatic degradation of poly (3-hydroxybutyrate)"Polymer. 41. 3227-3234 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Suzuki, T.Ohura, K.Kasuya, T.Toshima, Y.Doi, S.Matsumura: "Enzyme-catalyzed ring-opening polymerization of β-butyrolactone using PHB depolymerase"Chem. Lett.. 318-319 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Kasuya, H.Mitomo, M.Nakahara, A.Akiba, T.Kudo, Y.Doi: "Identification of a Marine Benthic P (3HB)-degrading Bacterium Isolate and Characterization of Its P (3HB) Depolymerase"Biomacromolecules. 1. 194-201 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Hiraishi, T.Ohura, S.Ito, K.Kasuya, Y.Doi: "Function of the Catalytic Domain of Poly (3-hydroxybutyrirate) Depolymerase from Pseudomonas stutzeri"Biomacromolecules. 1. 320-324 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.He, X.Shuai, A.Cao, K.Kasuya, Y.Doi, Y.Inoue: "Enzymatic biodegradation of chemosynthetic atactic P (3HB) enhanced by an amorphous nonbiodegradable polymer : blend of atactic P (3HB) with PMMA"Macromol. Rapid Commun.. 21. 1277-1281 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Miyazaki, K.Takahashi, M.Shiraki, T.Saito, Y.Tezuka, K.Kasuya: "Properties of a Poly (3-hydroxybtyrate) Depolymerase from Penicillium funiculosum"J. Polym Environ. 8. 175-182 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Mitomo, W.-C.Hsieha, K.Nishiwakia, K., Kasuya, Y.Doi: "Poly (3-hydroxybutyrate-co-4-hydroxybutyrate) Produced by Comamonas acidovorans"Polymer. 42. 3455-3461 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.He, X.Shuai, A.Cao, K.Kasuya, Y.Doi, Y.Inoue: "Enzymatic biodegradation of synthetic atactic poly (R, S-3-hydroxybutyrate) enhanced by an amorphous nonbiodegradable polymer"Polym. Degrad. Stab.. 73. 193-199 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Yoshie, Y.Oike, K.Kasuya, Y.Doi, Y.Inoue: "Change of surface structure of poly (3-hydroxybutyrate) film upon enzymatic hydrolysis by PHB depolymerase"Biomaclomolecules. 3. 1320-1326 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Wang, Y.Inagawa, Y.Osanai, K.Kasuya, T.Saito, S.Matsumura, Y.Doi, Y.Inoue: "Enzymatic hydrolysis of chemosynthesized atactic poly (3-hydroxyburyrate) by poly (3-hydroxyalkanoate) depolymerase from Acidovorax sp. TP4 and Ralstonia pickettii T1"Biomacromolecules. 3. 894-898 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Wang, Y.Inagawa, T.Saito, K.Kasuya, Y.Doi, Y.Inoue: "Enzymatic hydrolysis of bacterial poly (3-hydroxybutyrate-xo-3-hydroxypropionate) s by poly (3-hydroxyalkanoate) depolymerase from Acidovorax sp. TP4"Biomacromolecules. 3. 828-834 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] W.-C.Hiseh, K.Kasuya, H.Mitomo: "Crystallization and Evaluation of Physical Properties of Microbial Poly (3-hydroxybutyrate-co-4-hydroxybutyrate)"Sen-i Gakkaishi. 58. 428-431 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Kasuya, T.Takano, Y.Tezuka, W.-C.Hsieh, H.Mitomo, Y.Doi: "Cloning, Expression and Characterization of a Poly (3-hydroxybutyrate) Depolymerase from Marinobacter sp. NK-1"Int. J. Biol. Macromol. in press.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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