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2002 Fiscal Year Final Research Report Summary

Biodegradation of Bacterial Polyentens in Anerobic Conditions and is mechanisms (2002)

Research Project

Project/Area Number 11217214
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionKanagawa University

Principal Investigator

SAITO Terumi  Kanagawa Univ., Dept. of Science, Professor, 理学部, 教授 (80025717)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Teruyuki  Kanagawa Univ., Dept. of Science, Postdoctoral, 理学部, 博士研究員
Project Period (FY) 1999 – 2002
Keywordspoly (3-hydroxybatyrate) / polyhydroxy alkanoates / PHB depolymerase / PHB / inclusion bodies / intracellular PHB depolymerase / biodegradation / bacterial polyesters
Research Abstract

Several bacteria which degrade bacterial polyester (PHB) and also perform denitrification were isolated. We attempted the isolation of extracellular PHB depolymerases and cloning of their genes, but failed. When small fish (gold fish) were kept in the presence of PHB, a considerable reduction of nigrogen compounds in water was observed. These results indicate that the important role of the bacteria with ability of both PHB-degradation and denitrification in environment. On the other hand, we coned the gene of an intracellular PHB depolymerase (phaZl) from Ralstonia eutropha H16 and charaterization its product (PhaZ1). The amino acid sequence of PhaZ1 was novel. The molecular mass of PhaZ1 was 47 Kda. PhaZ1, which localized solely in PHB inclusions, degraded artificial amorphous PHB granules, but not crystalline PHB granules. We further cloned several intracellular 3-hydroxybutyrate-oligomer hydrolases. One of them, an intracellular 3HB-oligomer hydrolase (PhaZ2) from R. eutropha, showed both 3HB-oligomer hydrolase and PHB depolymerase activities, PhaZ2 localizedd both in cytosol and PHB inclusions, The amounts of PhaZ1 and PhaZ2 increased with the content of PHB in cells. The null mutant lacking both PhaZ1and PhaZ2 revealed the increase PHB deposition in cells. These results indicate that PhaZ1 and PhazZ2 cooperate in intracellular degradation of PHB

  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] T.Kobayashi他: "Biochemical and Genetic Characterization of an Extracellular an Extracellular Poly(3-hydroxybutyrate) Depolymerase from Acidovorax sp. Strain TP4"J.Environ.Polym.Degrad.. 7. 9-17 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Mitomo他: "Biosynthesis and Characterization of Poly (3-hydroxybutyrate-co-3-hydroxyvaAerate) Produced by Burkholderia ceoacia D1"In.J.Biol.Macromol.. 24. 311-318 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Miyazaki他: "roperties of a Poly(3-hydroxybutyrate) Depolymerase from Penicillium funiculosum"J.Polym.Environ.. 8. 175-182 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Saesusa他: "Cloning of an Intracellular Poly[D(-)-3-hydroxybutyrate] Depolymerase Gene from Ralstonia eutronha H16 and Characterization of the Gene Product"J.Bacteriol.. 183. 94-100 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Suzuki他: "Involvement of Catalytic Amino Acid Residues in Enzyme Catalyzed Polymerization for the Synthesis of Polyesters"Biomacromolecules. 2. 541-544 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Yamane他: "Enzymatic Degradation of Bacterial Homo-poly(3-hydroxybutyrate) Melt Spun Fibers"Polymer. 42. 7873-7878 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Inagawa他: "Identification and Characterization of Poly-3-hydrdroxybutyrate Granule-associated Protein, PGA12 and PGA16 in Zoogloea ramigera 1-16-M"Int.J.Biol.Macromol.. 30. 55-61 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Sugiyama他: "Cloning of an intracellular D(-)-3-Hydoxy utyrate-oligomer Hydrolase Gene from Acidovorax sp. Strain SA1 and Purification of the Enzyme"Current Microbiol.. 45. 123-127 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Saegusa他: "Cloning of an Intracellular D(-)-3-Hydoxybutyrate-oligomer Hydrolase Gene from R. eutropha H16 and Identification of the Active Site Serine Residue by Mutagenesis"Biosci.Biotechonol.Biochem.. 94. 106-112 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Wang他: "Enzymatic Hydrolysis of Bacterial Poly(3-hydroxyturyrate-co-3-Hydroxypropionate)s by Poly(3-hydroxy- alkanoate) Depolymerase from Acidovorax sp. TP4"Biomacromolecules. 3. 828-834 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y-H.Na他: "Phase-Separation Enhanced Enzymatic Degradation of tactic Poly(R, S-3-Hydroxybutyrate) in the Blends with Poly(methyl methaacrylate)"Polym.Degrad.Stab.. 79. 535-545 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kobayashi: "Purification and Properties of PhaZ2 in Ralstonia eutropha H16 and its identification as a Novel Intracellular Poly(3-hydroxybutyrate) Depolvmerase"J.Bacteriol.. 185. 3485-3490 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 齊藤光實他: "高分子の寿命予測と長寿命化技術,2章5節「微生物による劣化」"エヌ・ティ・エス. 7 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 粕谷健一他: "グリーンプラスチック最新技術 14章「生分解プラスチックの酵素分解」"シーエムシー. 12 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kobayashi, A.Sugiyama, Y.Kawase, T.Saito, J.Mergaert, J.Swings: "Biochemical and Genetic Characterization of an Extracellular an Extracellular Poly (3-hydroxybutyrate) Depolymerase from Acidovorax sp. Strain TP4"J.Environ. Polym. Degrad.. 7. 9-17 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Mitomo, T.Takahashi, H.Ito, T.Saito: "Biosynthesis and Characterization of Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) Produced by Burkholderia cepacia D1"Int. J. Biol. Macromol.. 24. 311-318 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Miyazaki, K.Takahashi, M.Shiraki, T.Saito, Y.Tezuka, K.Kasuya: "Properties of a Poly (3-hydroxybutyrate) Depolymerase from Penicillium funiculosum"J. Polym. Environ.. 8. 175-182 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Saegusa, M.Shiraki, C.Kanai, T.Saito: "Cloning of an Intracellular Poly [D(-)-3-hydroxybutyrate] Depolymerase Gene from Ralstonia eutropha H16 and Charaterization of the Gene Product"J. Bacteriol.. 183. 94-100 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Suzuki, S.Taguchi, T.Saito, K.Toshima, S.Matsumura, Y.Doi: "Involvement of Catalytic Amino Acid Residues in Enzyme-Catalyzed Polymerization for the Synthesis of Polyesters"Biomacromolecules. 2. 541-544 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Yamane, K.Terao, S.Hiki, Y.Kawahara, Y.Kimura, T.Saito: "Enzymatic Degradation of Bacterial Homo-poly (3-hydroxybutyrate) Melt Spun Fibers"Polymer. 42. 7873-7878 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Inagawa, Y.Inoue, M.Shiraki, T.Saito: "Identification and Characterization of Poly-3-hydroxybutyrate Granule-associated Protein, PGA12 and PGA16 in Zoogloea ramigera 1-16-M"Int. J. Biol. Macromol.. 30. 55-61 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Sugiyama, M.Shiraki, T.Kobayashi, G.Morikawa, M.Yamamoto, M.Yamaoka, T.Saito: "Cloning of an Intracellular D(-)-3-Hydoxybutyrate-oligomer Hydrolase Gene from Acidovorax sp. Strain SA1 and Purification of the Enzyme"Biosci. Biotechnol. Biochem.. 94. 106-112 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Saegusa, M.Shiraki, T.Saito: "Cloning of an Intracellular D(-)-3-Hydroxybutyrate-oligomer Hydrolalse Gene from Rlastonia eutropha H16 andd Identification of the Active Site Serine Residure by Site-Directed Mutagenesis"Biosci. Biotechnol. Biochem.. 94. 106-112 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Wang, Y.Inagawa, T.Saito, K.Kasuya, Y.Inoue: "Enzymatic Hydrolysis of Bacterial Poly (3-hydroxyturytate-co-3-Hydroxypropionate)s by Poly (3-hydroxy-alkanoate) Depolymerase from Acidovorax sp. TP4"Biomacromolecules. 3. 828-834 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y-H.Na, Y.He, T.Nishikawa, Y.Inagawa, Y.Osanai, S.Matsumura, T.Saito, Y.Doi, Y.Inoue: "Phase-Separation Enhanced Enzymatic Degradation of Atactic Poly (R, S-3-Hydroxybutyrate) in the Blends with Poly (methyl methaacrylate)"Polym. Degrad. Stab.. 79. 535-545 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kobayashi, M.Shiraki, T.Abe, A.Sugiyama, T.Saito: "Purification and Properties of an Intracellular 3-Hydroxybutyrate-Oligomer Hydrolase (PhaZ2) In Ralstonia eutropha H16 and Its Identification as a Novel Intracellular Poly (3-hydroxybutyrate) Depolymerase"J. Bacteriol.. 185. 3485-3490 (2003)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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