• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2002 Fiscal Year Final Research Report Summary

Mechanism for Developmental Regulation by Genomic Imprinting in the Female Germ Line.

Research Project

Project/Area Number 11234205
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionTokyo University of Agriculture

Principal Investigator

KONO Tomohiro  Faculty of Applied Bioscience, Professor, 応用生物化学部, 教授 (80153485)

Co-Investigator(Kenkyū-buntansha) OBATA Yayoi  Gene Research Centre, Gunma University, Assistant, 遺伝子実験施設, 助手 (70312907)
Project Period (FY) 1999 – 2002
KeywordsGenomic imprinting / Oocytes / Parthenogenesis / Gene expression / DNA methylation / Nuclear transfer
Research Abstract

In mammals, epigenetic modification of genomes during gametogenesis leads to an unequivalent expression of imprinted genes between parental alleles and necessary for term development. To understand the further insight into genomic imprinting we have done several lines of experiment. Firstly, to investigate when and how, precisely, maternal primary imprinting is established, we produced parthenogenetic embryos containing one genome from a non-growing or growth stage oocyte from 1- to 20-day old mice, and one from a fully-grown oocyte from adult mice. Using these embryos, expression analysis of eight imprinted genes, Snrpn, Znfl27, Ndn, Peg3, Igf2r, p57^<KIP2>, Peg1 and Impact, was conducted. The results show that the epigenetic markers for each imprinted gene are not imposed all together at a specific time during oocyte growth, but rather occurs within a wide range during the period from primary to antral follicle stage oocytes. The developmental ability of the constructed parthenogenet … More ic embryos is gradually reduced with the growth of nuclear donor oocytes. Thus, the present study clearly demonstrate that maternal primary imprinting occurs in a stepwise fashion for groups of imprinted genes during the oocyte growth stages. Secondly, we examined whether the regulation of H19 monoallelic expression enhances the survival of parthenogenetic embryos. The results clearly show that the ng/fg parthenogenetic embryos carrying the ng-oocyte genome that had been deleted by the H19 transcription unit successfully developed as live fetuses for 17.5 gestation days. Control experiments revealed that this unique phenomenon occurs irrespective of the genetic background effect. Histological analysis showed that the placenta of ng^<H19-KO>/fg^<WT> parthenotes was resulted in atrophia with sever negro-cytosis and other anomalies. The present results suggest that the cessation of H19 gene expression from the ng-allele causes the extended development and that functional defection of the placenta could be fatal for the ontogeny. Less

  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] Bao, S., Ushijima, H., Hirose, A., Fumihito, A., Ono, Y., Kono, T.: "Development of bovine oocytes reconstructed with a nucleus from growing stage oocytes after fertilization in vitro"Theriogenology. 59. 1231-1239 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimozawa, N., Ono, Y., Kimoto, S., Hioki, K., Araki, Y., Shinkai, Y., Kono, T., Ito, M.: "Abnormalities in cloned mice are not transmitted to the progeny"Genesis. 34. 203-207 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Obata, Y., Kono, T., Hatada, I.: "Maturation of mouse fetal germ cells in vitro"Nature. 418. 497-498 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimozawa, N.,, Muguruma, K., Hioki, K., Arai, Y., Kono, T., Ito, M.: "Direct production of gene-targeted mice from ES cells by nuclear transfer and gene transmission to their progeny"Exp. Anim.. 51. 375-381 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Bao, S., Obata, Y., Ono, Y., Futatsumata, N., Niimura, S., Kono, T.: "Nuclear competence for maturation and pronuclear formation in mouse oocytes"Human Reprod.. 17. 1311-1316 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sotomaru, Y., Katsuzawa, Y., Hatada, I., Obata, Y., Sasaki, H., Kono, T.: "Unregulated expression of the imprinted genes H19 and lgf2r in mouse uniparental fetuses"J. Blot. Chem.. 277. 12474-12478 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimoda, M., Morita, S., Obata, Y., Sotomaru, Y., Kono, T., Hatada, I.: "Imprinting of a small nucleolar RNA gene on mouse chromosome 12"Genomics. 79. 483-486 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kono, T., Sotomaru, Y., Katsuzawa, Y., L, Dandolo.: "Mouse parthenogenetic embryos with monoallelic H19 expression can develop today 17.5 of gestation"Dev. Biol.. 243. 294-300 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Obata, Y., Kono, T.: "Maternal primary imprinting is established at a specific time for each gene throughout oocyte growth"J. Biol. Chem.. 277. 5285-5289 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mizuno, Y., Sotomaru, Y., Katsuzawa, Y., Kono, T., Okazaki, Y., et al.: "Asb4,Ata3,and dcn are novel imprinted genes identified by high-throughput screening using RIKEN cDNA microarry"BBRC. 290. 1499-1505 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ono, Y., et al.: "Production of cloned mice from embryonic stem cells arrested at metaphase"Reproduction. 122. 731-736 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hatada, I., Morita, S., Obata, Y., Sotomaru, Y., Shimoda, M., Kono, T.: "Identification of a New Imprinted Gene, Rian, on Mouse Chromosome 12 by Fluorescent Differential Display Screening"J. Biochem.. 130. 187-190 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sotomaru, Y., Kawase, Y., Obata, Y., Hatada, I., Kono, T.: "Disruption of lmprinted Expression of U2afbp-rs/U2af1-rs1 Gene in Mouse Parthenogenetic Fetuses"J. Biol.Chem.. 276. 26694-26698 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sotomaru, Y., Katsuzawa, Y., Domeki, I., Kono, T.: "Determination by Real-Time RT-PCR of Imprinted Expression of the Insulin-Like Growth Factor II(lgf2)Gene in Mouse Uniparental Fetuses"J. Reprod. Dev.. 47. 139-144 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ono, Y., Shimozawa, N., Ito, M., Kono, T.: "Cloned mice from fetal fibroblast cells arrested at metaphase by a serial nuclear transfer"Biol. Reprod.. 64. 44-50 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ueda, T., Abe, K., Miura, A., Yuzuriha, M., et al.: "The paternal methylation imprint of the H19 locus is acquired in the gonocyte stage during fetal testis development"Genes to Cells. 5. 649-659 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tada, T., Obata, Y., Tada, M., Goto, Y., Nakatsuji, N., et al.: "Imprint switching for non-random X-chromosome inactivation during mouse oocyte growth"Development. 127. 3101-3105 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Obata, Y.Ono, Y., Akuzawa, H., Kwon, O.Y., Yoshizawa, M., Kono, T.: "Post-implantation developmental of mouse androgenetic embryos produced by in-vitro fertilization of enucleated oocytes"Hum. Reprod.. 15. 874-880 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Bao, S., Obata, Y., Carroll, J., Kono, T.: "Epigenetic modifications necessary for normal are established during oocyte growth in mice"Biol. Reprod.. 62. 616-621 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Bao S., et al.: "Development of bovine oocytes recon reconstructed with a nucleus from growing stage oocytes after fertilization in vitro"Theriogenology. 59. 1231-1239 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimozawa N., et al.: "Abnormalities in Cloned Mice Are Not Transmitted to the Progeny"Genesis. 34. 203-207 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] ObataY., et al.: "Maturation of mouse fetal germ cells in vitro"Nature. 418. 497-498 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimozawa, N., et al.: "Direct Production of Gene-targeted mice from ES cells by nuclear transfer and gene transmission to their Progeny"Exp. Anim.. 51. 375-381 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Bao, S.,et al.: "Nuclear competence for maturation and pronuclear formation in mouse oocytes"Hum Reprod. 17. 1311-1316 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sotomaru, Y., et al.: "Unregulated Expression of the Imprinted Genes H19 and Igf2r in Mouse Uniparental Fetuses"J. Biol. Chem.. 277. 12474-12478 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimoda, M.: "Imprinting of a Small Nucleolar RNA Gene on Mouse Chromosome 12"Genomics. 79. 483-486 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kono, T., et al.: "Mouse parthenogenetic embryos with monoallelic H19 expression can develop to day 17.5 of gestation"Dev. Biol.. 243. 294-300 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Obata, Y., Kono, T.: "Maternal primary imprinting is established at a cific time for each individual gene throughout oocyte growth"J. Biol. Chem.. 277. 5285-5289 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mizuno Y., et al.: "Asb4, Ata3, and Dcn Are Novel Imprinted Genes Identified by High-Throughput Screening Using RIKEN cDNA Microarry"BBRC. 290. 1499-1505 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ono, Y., et al.: "Production of cloned mice from embryonic stem cells arrested at metaphase"Reproduction. 122. 731-736 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hatada, I., et al.: "Identification of a New Imprinted Gene, Rian, on Mouse Chromosome 12 by Fluorescent Differential Display Screening"J. Biochem.. 130. 187-190 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sotomaru, Y., et al.: "Disruption of Imprinted Expression of U2afbp-rs/U2af1-rs1 Gene in Mouse Parthenogenetic Fetuses"J. Biol. Chem.. 276. 26694-26698 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sotomaru, Y., et al.: "Determination by Real-Time RT-PCR of Imprinted Expression of the Insulin-Like Growth Factor II(lgf2)Gene in Mouse Uniparental Fetuses"J. Reprod. Dev.. 47. 139-144 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ono, Y., et al.: "Cloned mice from fetal fibroblast cells arrested at metaphase by a serial nuclear transfer"Biol. Reprod.. 64. 44-50 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueda, T., et al.: "The paternal methylation imprint of the H19 locus is acquired in the gonocyte stage during fetal testis development"Genes to Cells. 5. 649-659 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tada, T., et al.: "Imprint switching for non-random X-chromosome inactivation during mouse oocyte growth"Development. 127. 3101-3105 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Obata, Y., et al.: "Post-implantation developmental of mouse androgenetic embryos produced by in-vitro fertilization of enucleated oocytes"Hum Reprod.. 15. 874-880 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Bao, S., wt al.: "Epigenetic modifications necessary for normal development are established during oocyte growth in mice"Biol. Reprod.. 62. 616-621 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2004-04-14  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi