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2003 Fiscal Year Final Research Report Summary

Roles of guanylyl cyclase system in adaptation to the environment

Research Project

Project/Area Number 11236202
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionHokkaido University

Principal Investigator

SUZUKI Norio  Hokkaido University, Graduate School of Science, Professor, 大学院・理学研究科, 教授 (20082133)

Co-Investigator(Kenkyū-buntansha) KUSAKABE Takehiro  Hokkaido University, Graduate School of Science, Research Associate, 大学院・理学研究科, 助手 (40280862)
Project Period (FY) 1999 – 2003
Keywordsmembrane guanylyl cyclase / soluble guanylyl cyclase / nitric oxide / atrial natriuretic peptide / in situ hybridization / morpholino / medaka fish / cGMP
Research Abstract

We demonstrated that binding of a protein with 17 kDa, which is a medaka fish homologue of mammalian co-activator "PC4", to the nucleotides (AGACCTTTGC) between-90 and -81 in the 5'-flanking region of the OIGC6 gene are essential for transcription of the OlGC6 gene in medaka fish intestine. In European eel, it has been demonstrated that expression of the GC-C2 transcript in the intestine was increased by 100% following the transfer of yellow fresh water-acclimated eels to sea water, while no changes in expression of OlGC9 were seen when adult medaka fish individuals were transferred to 500/0 sea water. Expression of neuronal NO synthase gene (nNOS), three different subunit genes (OlGCS-α_1, OlGCS-α_2, and OlGCS-β_1) of soluble GC, and three PKG genes (PKG 1α ; PKG 1β, and PKG II) were examined during embryogenesis of medaka fish with or without morpholino antisense oligonucleotide of respective gene. nNOS and OlGCS-β_1 were expressed matemally and OlGCS-α_1, OlGCS-α_2, PKG I, and PKG II were expressed zygotically. The zygotic expression of OlGCS-α_1 was detected at stage 19, while that of OlGCS-a2 was detected at stage 16. The Hong Kong-originated medaka fish Oryzias curvinotus expresses nine genes (OcGC1-OcGC8 and OcGC-R2) for membrane GCs and three genes (OcGCS-_1 OcGCS-_2, and OcGCS-_1) for soluble GC subunits. O. curvinotus was able to produce the hybrids with O. latipes irrespective of the direction of crossing and the resulting hybrids expressed both the matemal and patemal soluble GC subunit genes almost equally, suggesting the possibility of the formation of chimeric heterodimer in the hybrids.

  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] Fujie, S.: "Nitric oxide synthase and soluble guanylyl cyclase underlying the modulation of electrical oscillation in a central olfactory organ."J.Neurobiol. (In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yao, Y.: "Genomic structure and expression of the soluble guanylyl cyclase α_2 subunit gene in the medaka fish Oryzias latipes. Zool.Sci."Zoological Science. 20. 1293-1304 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Inoue, K.: "Four functionally distinct C-type natriuretic peptides found in fish reveal new evolutionary history of the natriuretic peptide system"Proc.Natl.Acad.Sci.USA. 100. 10079-10084 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamagami, S.: "Expression and, genomic organization of a medaka fish novel membrane form of guanylyl cyclase/orphan receptor."Zoological Science. 20. 591-606 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto, T.: "Localization of the NO1cGMP signaling pathway-related genes and influences of soluble GC molpholino Knock-down on medaka fish embryogenesis"Zoological Science. 20. 181-191 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Harumi, T.: "Expression of membrane-bound and soluble guanylyl cyclase mRNAs in embryonic and adult retina of the medaka fish Oryzias latipes."Zoological Science. 20. 133-140 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakauchi, M.: "Promoter analysis of a medaka fish intestinal guanylyl cyclase gene."FEBS Lett.. 536. 12-18 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kikukawa, Y: "The 26S proteasome Rpn10 gene encoding splicing isoforms : Evolutional conservation of the genomic organization in vertebrates"Biol.Chem.. 383. 1257-1261 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto, T.: "Promoter activity of the 5'-flanking regions of medaka fish soluble guanylyl cyclase α_1 and β_1, subunit genes."Biochem.J.. 361. 337-345 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kuroda, R.: "Increase of cGMP, cADP-ribose and inositol 1,4,5-trisphosphate preceding Ca^<2+> transients in fertilization of sea urchin eggs."Development. 128. 4405-4414 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tanabe, Y.: "Genomic structure and expression of the sea urchin soluble guanylyl cyclase β subunit gene."Zoological Science. 18. 811-817 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamagami, S.: "Expression and exon/intron organization of two medaka fish homologs of the mammalian guanylyl cyclase A."J.Biochem.. 130. 39-50 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Xu, S.H.: "Expression of sperm-activating peptide IV receptor-associated membrane guanylyl cyclase in the testis of the sea urchin Diadema setosum."Zoological Science. 18. 687-693 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kusakabe, T.: "A cis-regulatory element essential for photoreceptor cell-specific expression of a medaka retinal guanylyl cyclase gene."Dev.Genes Evol.. 211. 145-149 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ku sakabe, T.: "hotoreceptors and olfactory cells express the same retinal guanylyl cyclase isoform in medaka : visualization by promoter transgenics."FEBS Lett.. 483. 143-148 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nomura, K.: "False fertilization in sea urchin eggs induced by diabolin, a 120K kelp protein."Biochem.Biophys.Res.Commun.. 272. 691-693 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kusakabe, T.: "The guanylyl cyclase family in medaka fish Dryzias latipes."Zoological Science. 17. 131-140 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Naruse, K.: "A detailed linkage map of medaka, Oryzias latipes : Comparative genomics and genome evolution."Genetics. 154. 1773-1784 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shibata, Y.: "Identification and cDNA cloning of alveolin, an extracellular metalloproteinase, which induces chorion hardening of medaka (Oryzias latipes) eggs upon fertilization."J.Biol.Chem.. 275. 8349-8354 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiga, T., Suzuki, N.: "Expression of the guanylyl cyclase genes in the medaka fish hybrids between Oryzias curvinotus and Oryzias latipes."Submitted to Comp. Biochem. Physiol.. (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujie, S., Yamamoto, T., Murakami, J., Hatakeyama, H.Shiga, H., Suzuki, N., Ito, E.: "Nitric oxide synthase and soluble guanylyl cyclase underlying the modulation of electrical oscillation in a central olfactory organ."J. Neurobiol., in press..

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yao, Y., Yamamoto, T., Tsutsumi, M., Matsuda, M., Hori, H., Naruse, K., Mitani, H., Shima, A., Asakawa, S., Shimizu, N., Suzuki, N.: "Genomic structure and expression of the soluble guanylyl cyclase a_2 subunit gene in the medaka fish Oryzias latipes."Zool. Sci.. 20. 1293-1304 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inoue, K., Naruse, K., Yamagami, S., Mitani, H., Suzuki, N., Takei Y.: "Four functionally distinct C-type natriuretic peptides found in fish reveal new evolutionary history of the natriuretic peptide system."Proc. Natl. Acad. Sci. USA. 100. 10079-10084 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamagami, S., Xu, S.H., Suzuki, N.: "Expression and genomic organization of a medaka fish novel membrane form of guanylyl cyclase/orphan receptor."Zool. Sci.. 20(5). 591-606 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto, T., Yao, Y., Harumi, T., Suzuki, N.: "Localization of the NO/cGMP signaling pathway-related genes and influences of soluble GC molpholino Knock-down on medaka fish embryogenesis."Zool. Sci.. 20. 181-191 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Harumi, T., Watanabe, T., Yamamoto, T., Tanabe, Y., Suzuki, N.: "Expression of membrane-bound ad soluble guanylyl cyclase mRNAs in embryonic and adult retina of the medaka fish Oryzias latipes."Zool. Sci.. 20. 133-140 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakauchi, M., Suzuki, N.: "Promoter analysis of a medaka fish intestinal guanylyl cyclase gene."FEBS Lett.. 536. 12-18 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kikukawa, Y., Shimada, M., Suzuki, N., Tanaka, K., Yokosawa, H., Kawahara, H.: "The 26S proteasome Rpn10 gene encoding splicing isoforms : Evolutional conservation of the genomic organization in vertebrates."Biol. Chem.. 383. 1257-1261 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto, T., Suzuki, N.: "Promoter activity of the 5'-flanking regions of medaka fish soluble guanylyl cyclase a_1 and b_1 subunit genes."Biochem. J.. 361. 337-345 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuroda, R., Kontani, K., Kanda, Y., Katada, Nakano, T., Satoh, Y., Suzuki, N., Kuroda, H.: "Increase of cGMP, cADP-ribose and inositol 1,4,5-trisphosphate preceding Ca^<2+> transients in fertilization of"Development. 128. 4405-4414 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanabe, Y., Suzuki, N.: "Genomic structure and expression of the sea urchin soluble guanylyl cyclase b subunit gene."Zool. Sci.. 18. 811-817 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamagami, S., Suzuki, K., Suzuki, N.: "Expression and exon/intron organization of two medaka fish bomologs of the mammalian guanylyl cyclase A."J. Biochem.. 130. 39-50 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Xu, S.H., Yamagami, S., Nomura, K., Suzuki, N.: "Expression of sperm-activating peptide IV receptor-associated membrane guanylyl cyclase in the testis of the sea urchin Diadema setosum."Zool. Sci.. 18. 687-693 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kusakabe, T., Suzuki, N.: "A cis-regulatory element essential for photoreceptor cell-specific expression of a medaka retinal guanylyl cyclase gene."Dev. Genes Evol.. 211. 145-149 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kusakabe, T., Suzuki, N.: "Photoreceptors and olfactory cells express the same retinal guanylyl cyclase isform in medaka : visualization by promoter transgenics."FEBS Lett.. 483. 143-148 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nomura, K., Nakamura, H., Suzuki N.: "False fertilization in sea urchin eggs induced by diabolin, a 120K help protein."Biochem. Biophys. Res. Commun.. 272. 691-693 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kusakabe, T., Suzuki N.: "The guanylyl cyclase family in medaka fish Oryzias latipes."Zool. Sci.. 17. 131-140 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Naruse, K., Fukamachi, S., Mitani, H., Kondo, M., Matsuoka, T., Kondo, S., Hamamura, N., Morita, Y., Hasegawa, K., Mishigaki, R., Shimada, A., Wada, H., Husakabe, T., Suzuki, N., Kinoshita, M., Kanamori, A., Terado, T., Kimura, H., Nonaka, M., Shima, A.: "A detailed l, inkage map of medaka, Oryzias latipes : Comparative genomics and genome evolution."Genetics. 154. 1773-1784 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Shibata, T.Iwamatsu, Y.Oba, D.Kobayashi, M.Tanaka, Y.Nagahama, N.Suzuki, M.Yoshikuni: "Identification and cDNA cloning of alveolin, an extracellular metalloproteinase, which induces chorion hardening of medaka (Oryzias latipes) eggs upon"J. Biol. Chem.. 275. 8349-8354 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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