2003 Fiscal Year Final Research Report Summary
High density mapping of BAC end sequences onto medaka linkage groups
| Project/Area Number |
11236210
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Hokkaido University (2000-2003)
Okazaki National Research Institutes (1999) |
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Principal Investigator |
TANAKA Minoru Hokkaido University, Graduate School of Science, Associate Professor, 大学院・理学研究科, 助教授 (80202175)
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| Project Period (FY) |
1999 – 2003
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| Keywords | medaka / genome / typing / STS marker / physical mapping / linkage / recombination |
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| Research Abstract |
Physical mapping of medaka chromosomes was constructed based on the length polymorphism between two inbred strains, HNI and AA2. Fluorescent primers designed by BAC end sequences and nucleotide sequences having (CA) repeats were generated. 607 primer sets showing length polymorphism between the two strains were selected and were further used for typing to map onto the medaka linkage groups.
More than 15% of the primers generated showed length polymorphism, confirming the high genomic polymorphism between the two strains.
This information is the first systematic STS markers on medaka genomes and provides a powerful tool for anchoring BAC onto the linkage groups and for walk to the region of interest.
Estimated physical length do not correspond to that estimated by anonymous markers such as RFLP and RAPD. Especially the length of linkage group 23 is estimated to be much longer than that estimated before. Regions of repression of recombination are different between female and male. It is inclined that recombination in male occurs more frequently at the distal regions of the linkages.
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[Publications] Naruse, K., Tanaka.M., Mita, K., Shima, A., Postlethwait, J., Mitani, H.: "A Medaka Gene Map : The Trace of Ancestral Vertebrate Proto-chromosomes Revealed by Comparative Gene Mapping."Genome Research, (in press). (2004)
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「研究成果報告書概要(欧文)」より
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[Publications] Okubo, K., Ishii, S., Ishida, J., Mitani, H., Naruse, K., Kondo, M., Shima, A., Tanaka, M.Asakawa, S., Shimizu, N., Aida, K.: "A novel third gpnadotropin-releasing hormone receptor in the medaka Oryzias latipes : veolutionary and functional implications."Gene. 314. 121-131 (2003)
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[Publications] Tanaka, M., Nakajin, S., Kobayashi, D., Fukada, S., Guan, G., Todo, T., Senthikumaran, B., Nagahama, Y.: "Teleost ovarian carbonyl reductase-like 20b-hydroxysteroid dehydrogenase : potential role in the production of maturation-inducing hormone during final ooccyte maturation."Biol. Reprod.. 66. 1498-1504 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Okubo, K., Mitani, H., Naruse, K., Kondo, M., Shima, A., Tanaka, M., Aida, K.: "Conserved physical linkage of GnRH-R and RBM8 in the medaka and human genome."Biochem. Biophys. Res. Com.. 293. 327-331 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Yokoi, H., Kobayashi, T., Tanaka, M., Nagahama, Y., Wakamatsu, Y., Takeda, H., Araki, K., Morohashi, K., Ozato, K.: "Sox9 in a teleost fish, medaka (Oryzias latipes) : evidence for diversified function of sox9 in gonad differentiation."Mol. Reprod. Develop.. 63. 5-16 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Kondo, M, Froschauer, A, Kitano, A, Nanda, I, Hornung, U, Volff, J-N, Asakawa, S, Mitani, H, Naruse, K, Tanaka, M., Schmid, M, Shimizu, N., Schartl, M., Shima, A.: "Molecular cloning and characterization of DMRT genes from the medaka Oryzias latipes and the platyfish Xiphophorus maculatus"Gene. 295. 213-222 (2002)
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[Publications] Shinomiya, A., Tanaka, M., Kobayashi, T., Nagahama, Y., Hamaguchi, S.: "The vasa-like gene, olvas, identifies the migration path of primordial germ cells during embryonic body formation stage in the medaka, Oryzias latipes."Develop. Growth Differ.. 42. 317-326 (2000)
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