2002 Fiscal Year Annual Research Report

高等植物の自家不和合性における自他識別機構の分子的解析

Research Project

Project/Area Number	11238101
Research Institution	Nara Institute of Science and Technology
Principal Investigator	磯貝彰奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (20011992)
Co-Investigator(Kenkyū-buntansha)	鈴木昭憲秋田県立大学, 学長 (90011907) 崎山文夫大阪大学, 名誉教授 (40029947) 神山康夫三重大学, 生物資源学部, 教授 (80024579) 日向康吉東北大学, 名誉教授 (00005589) 村田紀夫岡崎共同研究機構, 基礎生物学研究所, 教授 (90011569)
Keywords	自家不和合性 / S遺伝子 / RNase / レセプターキナーゼ / F-box蛋白質 / 自他識別
Research Abstract	本研究は、特定領域研究(B)「高等植物の自家不和合性における自他識別機構の分子的解析」5つの計画研究を統括し、調整し、推進するために策定された。本年度は、配偶体型自家不和合性の花粉因子の解明を研究全体の最大の課題と考え、総括班経費でPD研究者を雇用し、個別研究課題を援助した。その結果、配偶体型自家不和合性を示し、ゲノムサイズの小さいことが知られているバラ科ウメを材料として、S-RNase近傍の遺伝子を解析したところ、S-RNaseに連鎖するSLF (S-locus F-box)遺伝子を見いだすことができた。この遺伝子は、花粉で発現し、しかもハプロタイプ間で多様性を示す。また、これをもとに、ペチュニアでもSLG遺伝子を見いだした。このことから、配偶体型自家不和合性では、自己以外のS-RNaseは花粉管内で、SLF蛋白質が関与するユビキチン化により、プロテアソーム系で分解されるという分子機構を呈示することができた。又、特筆すべきこととして、本研究の評価担当者の日向康吉と研究代表者磯貝彰の「アブラナ科植物の自家不和合性にかかわる自他識別機構の研究」(共同研究)にたいして、本年度の日本学士院賞が授与された。本年度の各研究の進展状況は、配偶体型グループ、胞子体型グループが個別に共同研究の推進をかねて別々に行い、その進捗状況と今後の方針を確認した。また、本総括班が主体となって、日本植物生理学会第43回シンポジウムにおいて、1セッションを企画し、内外の研究者に広く、その成果を発表した。また、その成果の全体のとりまとめとして、昨年度同様、個別の研究の成果の概要と印刷された論文集をまとめた成果報告書を作成し、関係各方面に配布した。

Research Products

(19 results)

All Other

All Publications (19 results)

[Publications] H.Shiba: "The dominance of alleles controlling self-incompatibility Brassica pollen is regulated at the RNA level"Plant Cell. 14. 491-504 (2002)
[Publications] S.Takayama: "Peptide signalling in plants"Curr. Opin. Plant Biol.. 5. 382-387 (2002)
[Publications] A.Isogai: "Molecular mechanism for the recognition reaction in the self-incompatibility of Brassica species"Proc. Japan Acad.. 78,Ser.B. 241-249 (2002)
[Publications] S.Takayama: "Molecular mechanism of self-recognition in Brassica self-incompatibility"J. Exp. Bot.. 54. 149-156 (2003)
[Publications] T.Kakizaki: "Linear dominance relationship among four class-II S haplotypes in pollen is determined by the expression of SP11 in Brassica se1f-incompatibility"Plant Cell Physiol.. 44. 70-75 (2003)
[Publications] H.Shiba: "Genomic organization of the S-locus region of Brassica"Biosci. Biotechnol. Biochem.. (in press).
[Publications] T.Entani: "Comparative analysis of the self-incompatibility (S-) locus region of Prunus mume : identification of a pollen-expressed F-box gene with allelic diversity"Genes Cells. (in press).
[Publications] G.Suzuki: "The S haplotypes lacking SLG in the genome of Brassica rapa"Plant Cell Rep.. (in press).
[Publications] M.Iwano: "Immunohistochemical studies on translocation of pollen S-haplotype determinant in self-incompatibility of Brassica rapa"Plant Cell Physiol.. (in press).
[Publications] Y.Sato: "Commonalty of self-recognition specificity of S haplotypes between Brassica oleracea and Brassica rapa"Plant Mol. Biol.. (in press 2003).
[Publications] R.Fujimoto: "Identification of S haplotypes in Brassica by dot blot analysis of SP11 alleles"Theor. Appl. Genet.. (in press 2003).
[Publications] R.Inaba: "Phylogenetic analysis of Brassiceae based on the nucleotide sequences of the S-locus related gene, SLR1"Theor. Appl. Genet.. 105. 1159-1165 (2002)
[Publications] K.Sato: "Coevolution of the S-locus genes SRK, SLG, and SP11/SCR in Brassica oleracea and B. rapa"Genetics. 162. 931-940 (2002)
[Publications] Y.Sato: "Efficient detection of DNA polymorphism in cabbage and rice cultivars by PCR-RF-SSCP (PRS)"Plnat Cell Rep.. 21. 276-281 (2002)
[Publications] K.Kondo: "Cultivated tomato has defects in both S-RNase and HT genes required for stylar function of self-incompatibility"Plant J.. 29. 627-636 (2002)
[Publications] K.Kondo: "Insights into the evolution of self-compatibility in Lycopersicon from a study of stylar factors"Plant J.. 30. 143-153 (2002)
[Publications] C.Castillo: "Cloning of the S8-RNase (S8-allele) of Japanese pear (Pyrus pyrifolia Nakai)"Plant Biotechnology. 19. 1-6 (2002)
[Publications] D.Kiyozumi: "Pollen UDP-glucose pyrophosphorylase showing polymorphism well-correlated to S genotype of Pyrus pyrifolia, Japanese pear"Sex. Plant Reprod.. 14. 315-323 (2002)
[Publications] 磯貝彰: "植物は自分の花粉をどうやって見分けるか-アブラナ科植物の自家不和合性の分子機構"蛋白質核酸酵素. 47(6). 700-707 (2002)

2002 Fiscal Year Annual Research Report

高等植物の自家不和合性における自他識別機構の分子的解析

Principal Investigator

磯貝 彰 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (20011992)

Research Products

[Publications] H.Shiba: "The dominance of alleles controlling self-incompatibility Brassica pollen is regulated at the RNA level"Plant Cell. 14. 491-504 (2002)

[Publications] S.Takayama: "Peptide signalling in plants"Curr. Opin. Plant Biol.. 5. 382-387 (2002)

[Publications] A.Isogai: "Molecular mechanism for the recognition reaction in the self-incompatibility of Brassica species"Proc. Japan Acad.. 78,Ser.B. 241-249 (2002)

[Publications] S.Takayama: "Molecular mechanism of self-recognition in Brassica self-incompatibility"J. Exp. Bot.. 54. 149-156 (2003)

[Publications] T.Kakizaki: "Linear dominance relationship among four class-II S haplotypes in pollen is determined by the expression of SP11 in Brassica se1f-incompatibility"Plant Cell Physiol.. 44. 70-75 (2003)

[Publications] H.Shiba: "Genomic organization of the S-locus region of Brassica"Biosci. Biotechnol. Biochem.. (in press).

[Publications] T.Entani: "Comparative analysis of the self-incompatibility (S-) locus region of Prunus mume : identification of a pollen-expressed F-box gene with allelic diversity"Genes Cells. (in press).

[Publications] G.Suzuki: "The S haplotypes lacking SLG in the genome of Brassica rapa"Plant Cell Rep.. (in press).

[Publications] M.Iwano: "Immunohistochemical studies on translocation of pollen S-haplotype determinant in self-incompatibility of Brassica rapa"Plant Cell Physiol.. (in press).

[Publications] Y.Sato: "Commonalty of self-recognition specificity of S haplotypes between Brassica oleracea and Brassica rapa"Plant Mol. Biol.. (in press 2003).

[Publications] R.Fujimoto: "Identification of S haplotypes in Brassica by dot blot analysis of SP11 alleles"Theor. Appl. Genet.. (in press 2003).

[Publications] R.Inaba: "Phylogenetic analysis of Brassiceae based on the nucleotide sequences of the S-locus related gene, SLR1"Theor. Appl. Genet.. 105. 1159-1165 (2002)

[Publications] K.Sato: "Coevolution of the S-locus genes SRK, SLG, and SP11/SCR in Brassica oleracea and B. rapa"Genetics. 162. 931-940 (2002)

[Publications] Y.Sato: "Efficient detection of DNA polymorphism in cabbage and rice cultivars by PCR-RF-SSCP (PRS)"Plnat Cell Rep.. 21. 276-281 (2002)

[Publications] K.Kondo: "Cultivated tomato has defects in both S-RNase and HT genes required for stylar function of self-incompatibility"Plant J.. 29. 627-636 (2002)

[Publications] K.Kondo: "Insights into the evolution of self-compatibility in Lycopersicon from a study of stylar factors"Plant J.. 30. 143-153 (2002)

[Publications] C.Castillo: "Cloning of the S8-RNase (S8-allele) of Japanese pear (Pyrus pyrifolia Nakai)"Plant Biotechnology. 19. 1-6 (2002)

[Publications] D.Kiyozumi: "Pollen UDP-glucose pyrophosphorylase showing polymorphism well-correlated to S genotype of Pyrus pyrifolia, Japanese pear"Sex. Plant Reprod.. 14. 315-323 (2002)

[Publications] 磯貝 彰: "植物は自分の花粉をどうやって見分けるか-アブラナ科植物の自家不和合性の分子機構"蛋白質核酸酵素. 47(6). 700-707 (2002)

磯貝彰奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (20011992)

[Publications] 磯貝彰: "植物は自分の花粉をどうやって見分けるか-アブラナ科植物の自家不和合性の分子機構"蛋白質核酸酵素. 47(6). 700-707 (2002)