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2001 Fiscal Year Final Research Report Summary

Research on the plant response to A1 stress and the production of Al tolerant plant

Research Project

Project/Area Number 11306006
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

MATSUMOTO Hideaki  Res. Inst. For Bioresources, Okayama Univ., Professor, 資源生物科学研究所, 教授 (80026418)

Co-Investigator(Kenkyū-buntansha) EZAKI Bunichi  Res. Inst. For Bioresources, Okayama Univ., Assistant Professor, 資源生物科学研究所, 助手 (90243500)
YAMAMOTO Yoko  Res. Inst. For Bioresources, Okayama Univ., Associate Professor, 資源生物科学研究所, 助教授 (50166831)
Project Period (FY) 1999 – 2001
KeywordsAcid soil / Aluminum stress / Aluminum tolerant gene / Malate exclusion / Transgenic rice / Aluminum injury / Mitochondrial function / Reactive oxygen species
Research Abstract

The major constraint to plant growth in acid soils is the presence of toxic aluminum (Al) cations which inhibit root elongation. The enhanced Al tolerance exhibited by some cultivars of wheat is associated with the Al-dependent efflux of malate from the root apices. Malate forms a stable complex with Al which is harmless to plants, and therefore this efflux of malate forms the basis of a hypothesis to explain Al tolerance in wheat. We carried out the cloning of a wheat gene, ALMT1, that co-segregates with Al tolerance in F_2 and F_3 populations derived from crosses between near-isogenic wheat lines that differ in Al tolerance. The ALMT1 gene encodes a membrane protein that is constitutively expressed in the root apices of the AI-tolerant line at greater levels than in the near-isogenic but Al-sensitive line. Heterologous expression of ALMT1 in Xenopus oocytes, rice and cultured tobacco cells conferred an Al-activated malate efflux.
Potential mechanisms of AI toxicity measured as Al-indu … More ced inhibition of growth in cultured tobacco cells (Nicotiana tabacum, nonchlorophyllic cell line SL) and pea (Pisum sativum) roots were investigated. The accumulation of Al in tobacco cells caused instantaneously the repression of mitochondrial activities and, after a lag of about 12 h, triggered reactive oxygen species (ROS) production, respiration inhibition, ATP depletion, and the loss of growth capability almost simultaneously. The presence of an antioxidant, butylated hydroxyanisol, during Al treatment of SL cells prevented not only ROS production but also ATP depletion and the loss of growth capability, suggesting that the AI-triggered ROS production seems to be a cause of ATP depletion and the loss of growth capability. Furthermore, these three late events were similarly repressed in an Al-tolerant cell line (ALT301) isolated from SL cells, suggesting that the acquisition of antioxidant functions mimicking burylated hydroxyanisol can be a mechanism of Al tolerance. We conclude that A1 affects mitochondrial functions, which leads to ROS production, probably the key critical event in Al inhibition of cell growth. Less

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Matsumoto, H.: "Cell biology of aluminum toxicity and tolerance in higher plants"Int.Rev.Cytol.. 200. 1-46 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sivaguru, M., Fujiwara, T., Samaj, J., Baluska, F., Yang, Z.M., Osawa, H., Maeda, T., Mori, T., Volkmann, D., Matsumoto, H.: "Aluminum-induced 1→3-β-D-glucan inhibits cell-to-cell trafficking of molecules through plasmodesmata : A new mechanism of aluminum toxicity in plants"Plant Physiol.. 124. 991-1005 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yang, Z.M., Sivaguru, M., Horst, W.J., Matsumoto, H.: "Aluminum tolerance is achieved by exudation of citric acid from roots of soybean (Glycine max)"Physiol.Plant.. 110. 72-77 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Devi, S.R., Yamamoto, Y., Matsumoto, H.: "Isolation of aluminum-tolerant cell lines of tobacco in a simple calcium medium and their responses to aluminum"Physiol.Plant.. 112. 397-402 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osawa, H., Matsumoto, H.: "Possible involvement of protein phosphorylation in aluminum-responsive malate efflux from wheat root apex"Plant Physiol.. 126. 411-420 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto, Y., Kobayashi, Y., Matsumoto, H.: "Lipid peroxidation is an early symptom triggered by aluminum, but not the primary cause of elongation inhibition in pea roots"Plant Physiol.. 125. 199-208 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsumoto, H., Yamamoto, Y., Devi, S.R.: "Metals in the Environment : Analysis by Biodiversity, Prasad, M.N.V.(ed)"Marcel Dekker, Inc.. 31 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsumoto, H: "Cell biology of aluminum toxicity and tolerance in higher plants"Int. Rev. Cytol.. 200. 1-46 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sivaguru, M., Fujiwara, T., Samaj, J., Baluska, F., Yang, Z. M., Osawa, H., Maeda, T., Mori, T.,Volkmann, D. and Matsumoto, H.: "Aluminum-induced 1 → 3-β-D-glucan inhibits cell-to-cell trafficking of molecules through plasmodesmata : A new mechanism of aluminum toxicity in plants."Plant Physiol.. 124. 991-1005 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yang, Z. M., Sivaguru, M., Horst, W. J. and Matsumoto, H: "Aluminium tolerance is achieved by exudation of citric acid from roots of soybean (Glycine max)."Physiol. Plant.. 110. 72-77 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Devi, S. R., Yamamoto, Y. and Matsumoto, H.: "Isolation of aluminum-tolerant cell lines of tobacco in a simple calcium medium and their responses to aluminum."Physiol. Plant.. 112. 397-402 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Osawa, H. and Matsumoto, H.: "Possible involvement of protein phosphorylation in aluminum-responsive malate efflux from wheat root apex."Plant. Physiol.. 126. 411-420 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto, Y., Kobayashi, Y. and Matsumoto, H.: "Lipid peroxidation is an early symptom triggered by aluminum, but not the primary cause of elongation inhibitionin pea roots."Plant.Physiol.. 125. 199-208 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumoto, H., Yamamoto, Y. and Devi, S. R., M. N. V.(ed).: "Aluminum toxicity in acid soils: Plant response to aluminum. In Metals in the Environment Analysis by Biodiversity"Marcel Dekker ,Inc.. 289-319 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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