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2002 Fiscal Year Final Research Report Summary

Mechanisms of Reproductive Toxicity by Endocrine Disrupting Chemicals

Research Project

Project/Area Number 11307007
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Public health/Health science
Research InstitutionKansai Medical University

Principal Investigator

TOKUNAGA Rikio  Kansai Medical University Faculty of Medicine Professor, 医学部, 教授 (40121959)

Co-Investigator(Kenkyū-buntansha) ENDO Yoko (ICHIKAWA Yoko)  Kansai Medical University Faculty of Medicine Assistant Professor, 医学部, 講師 (50193438)
KOHNO Hirao  Kansai Medical University Faculty of Medicine Assistant Professor, 医学部, 講師 (30148522)
Project Period (FY) 1999 – 2002
KeywordsEndocrine Disrupting Chemicals / Reproductive Toxicity / Estrogen
Research Abstract

To evaluate the effects of endocrine disrupting chemicals on reproductive functions in human beings, we focused on phytoestrogens that are taken up through food intake. We tested the estrogenic and antiestrogenic effects of two phytoestrotens, resveratrol and naringenin. Resveratrol is reported to act as a "super agonist" in human MCF-7 cells, which was confirmed in the present study. However, in HeLa cells or COS-7 cells, the maximal activation by resveratrol was lower than that by estradiol. In yeast, the resveratrol concentration required for the maximal activation was 50-fold higher than in MCF-7 cells, while there was no difference in optimal estradiol concentrations. Difference in optimal concentration for the maximal activation was observed also observed for naringenin. Whether resveratrol acts as an antagonist was investigated. No antagonistic activity was detected with resveratrol in all reporter assay systems we used. Resveratrol and estradiol exhibited an additive effect in COS-7 or yeast cells, whereas synergistic effect was observed in 293 cells. In contrast to the complex interactions between resveratrol and estradiol, naringenin and estradiol exhibited additive effect in all reporter assay systems we used including yeast. Our study indicates that estrogenic and antiestrogenic activity of structurally diverse phytoestrogens is complex and unpredictable. To screen the estrogenic ingredients, possibly found in beverages, we prepared the extracts from beer hops and herb tea mixtures and determined their estrogenic activities. Two hops and four herb tea mixtures gave positive results. The common ingredients contained only in those herb tea mixtures was liquorice root.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Taketani S: "Structure and transcriptional regulation of the mouse ferrochelatase gene"Gene. 227. 117-124 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masuya Y: "MAP kinase-independent induction of proto-oncogene c-fos mRNA by hemin in human cells"Biochem Biophs Res Commun. 260. 289-295 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mohri T: "Activated Rac1 selectively up-regulates the expression of integrin a6b4 and induces cell adhesion and membrane ruffles of nonadherent colon cancer Colo201 cells"Exp Cell Res. 253. 533-540 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oyaizu Y: "A crucial role of caspase 3 and caspase 8 in paclitaxel-induced apotosis"Mol Cell Biol Res Commun. 2. 36-41 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Adachi Y: "Apotosis of colorectal adenocarcinoma induced by 5-FU and/or IFN-gamma through caspase 3 and caspase 8"Int J Oncol. 15. 1191-1196 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishihara R: "ELISA for rinary trehalase with monoclonal antibodies : a technique for assessment of renal tubular damage"Clin Chem. 46. 636-643 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikebukuro K: "Morphological change, loss of deltapsi(m) and activation of caspases upon apoptosis of colorectal adenocarcinoma induced by 5-FU"Cancer Lett. 153. 101-108 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 河野比良夫: "内分泌撹乱化学物質(環境ホルモン)問題について"生物試料分析. 23. 385-392 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furukawa T: "Involvement of PLAGL2 in activation of iron deficient-and hypoxia-induced gene expression in mouse cell lines"Oncogene. 20. 4718-4727 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Taketani S: "Structure and transcriptional regulation of the mouse ferrochelatase gene"Gene. 227. 117-124 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masuya Y: "MAP kinase-independent induction of proto-oncogene c-fos mRNA by hemin in human cells"Biochem Biophs Res Commun. 260. 289-295 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mohri T: "Activated Rac1 selectively up-regulates the expression of integrin a6b4 and induces cell adhesion and membrane ruffles of nonadherent colon cancer Colo201 cells"Exp Cell Res. 253. 533-540 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oyaizu Y: "A crucial role of caspase 3 and caspase 8 in paclitaxel-induced apoptosis"Mol Cell Biol Res Commun. 2. 36-41 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Adachi Y: "Apoptosis of colorectal adenocarcinoma induced by 5-FU and/or IFN-gamma through caspase 3 and caspase 8"Int J Oncol. 15. 1191-1196 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishihara R: "ELISA for rinary trehalase with monoclonal antibodies: a technique for assessment of renal tubular damage"Clin Chem. 46. 636-643 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanabe A: "Analysis of the candidate genes responsible for non-syndromic cleft lip and palate in Japanese people"Clin Sci. 99. 105-111 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikebukuro K: "Morphological change, loss of deltapsi(m) and activation of caspases upon apoptosis of colorectal adenocarcinoma induced by 5-FU"Cancer Lett. 153. 101-108 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Furukawa T: "Involvement of PLAGL2 in activation of iron deficient- and hypoxia-induced gene expression in mouse cell lines"Oncogene. 20. 4718-4727 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14   Modified: 2021-11-22  

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