2002 Fiscal Year Final Research Report Summary
Genome Scan of Candidate Region of Schizophrenia using DNA Pooling Method
| Project/Area Number |
11307014
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | Teikyo University |
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Principal Investigator |
NANKO Shinichiro Teikyo University, Department of Medicine, Professor, 医学部, 教授 (60101127)
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| Co-Investigator(Kenkyū-buntansha) |
TOKUNAGA Katsushi The University of Tokyo, Graduate School of Medicine and Faculty of Medicine, Professor, 医学系大学院, 教授 (40163977)
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| Project Period (FY) |
1999 – 2002
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| Keywords | schizophrenia / genome scan / chromosome 1 / ΔAIP / pooling / chromosome 9 |
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| Research Abstract |
One of the major strategies for identifying genes susceptible to illness is allelic association. The allelic association, however, would need to be genotyped hundreds of individuals of patients and controls with DNA markers. Therefore, large scale systematic searches for allelic association are costly and time consuming. Recently, an association approach called DNA pooling was developed to conduct a systematic search through the genome. In DNA pooling method, the allele image patterns (AIPs) for two created pools of DNA for cases and controls, instead of allele counts of individuals of patients and controls, were compared.
In this study, we have screened twelve DNA markers on chromosome 9 and 31 markers on chromosome 1 using DNA pooling method. Two markers on chromosome 9 were also genotyped individually in patient and control samples. The resulting pooled images obtained by GENE SCAN from automated DNA sequencer were overlaid in GENOTYPER. Calculation of ΔAIP was according to Daniels et. al (Am. J. Hum.Genet. 1998), I.e. ΔAIP=Dif/(Dif+Com). ΔAIP P value was calculated according to the program from P.H. on request.
The ΔAIP P values obtained from DNA pooling method for loci VLDLR and D9S15 were smaller than the X^2 P values from individual genotyping, though the ΔAIP analysis for D9S15 was a false positive.
The results suggested DNA pooling method for association studies could be used simply as a rapid initial screening procedure prior to individual genotyping. It is also suggested that the region tested here could be excluded from the candidate loci for schizophrenia. However, ten markers on chromosome 1 get large ΔAIP value although showed almost equal images between patients and controls, suggesting more technical problems should be overcome for this pooling methods.
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