1999 Fiscal Year Annual Research Report

神経可塑性と脳の発生・分化におけるIP_3受容体/Ca^<2+>シグナリングの役割

Research Project

Project/Area Number	11308032
Research Category	Grant-in-Aid for Scientific Research (A)
Research Institution	The University of Tokyo
Principal Investigator	御子柴克彦東京大学, 医科学研究所, 教授 (30051840)
Keywords	IP_3 / IP_3受容体 / 神経成長円錐 / LTD / LTP / 神経可塑性 / レーザー / Ca^<2+>
Research Abstract	今回は神経可塑性(神経成長円錐の形成、小脳・海馬における長期抑圧(LTD)と長期増強(LTP))や脳の発生・分化(細胞の極性の形成、シュペーマンオーガナイザーなどによる神経誘導など)にかかわるIP3受容体を介するCa2+波やCa2+振動の役割の解明を試みた。まず、レーザー光を利用した機能分子不活性化法[CALI(chromophore assisted laser inactivation]による神経成長円錐の形成及び脳の発生・分化のメカニズムの解析をした。特異抗体(これにマラカイトグリーンという色素をラベルする)を細胞内へ導入して、レーザー光照射により、ラジカルを放出させて、特異的に標的である抗原分子を破壊して、細胞内の一部に照射する方法のみならず、ビッグレーザーを利用して、レーザー光を利用してみた。そして組織レベル及び個体レベルへの応用を試みた。IP3吸収分子(IP3受容体のIP3結合ドメインに変異を加えることにより結合親和性が1000倍高い)の過剰発現を利用することで、IP3の発生・分化及び生理機能への役割をニューロン内へ導入した際の細胞内Ca2+動態と、機能・形態への影響、発生過程でのシュペーマン形成体の役割が明らかになった。具体的に各細胞群へ導入することによりCa2+と神経誘導、発生・分化との関連を調べた。

Research Products

(17 results)

All Other

All Publications (17 results)

[Publications] Yoshikawa, F. et al.: "Cooperative formation of the ligand-binding site of the inositol"J. Biol. Chem.. 274. 328-334 (1999)
[Publications] Yoshikawa, F. et al.: "Trypsinized cerebellar inositol 1,4,5-trisphosphate receptor"J. Biol. Chem.. 274. 316-327 (1999)
[Publications] Zhu. C. et al.: "Inosiotl 1,4,5-trisphosphate receptor down-regulation is activated"J. Biol. Chem.. 274. 3476-3489 (1999)
[Publications] Yoshikawa, F. et al.: "High efficient expression of the functional ligand binding site of the"Biochem. Biophys. Res. Commun.. 257. 792-797 (1999)
[Publications] Konishi. Y et al.: "Transcriptional regulation of mouse type 1 inositol 1,4,5-trisphosphate"J. Neurochem.. 72. 1717-1724 (1999)
[Publications] Ohkawa, N. et al.: "Activation of the mouse inositol 1,4,5-triphosphate receptor type 1"Gene. 229. 11-19 (1999)
[Publications] Hamada, T. et al.: "The role of inositol triphosphate-induced Ca2+ release from IP3-"Neurosience. Letters. 263. 125-128 (1999)
[Publications] Natsume, T. et al.: "Real time analysis of interaction between inositol 1,4,5-triphosphate"Biochem. Biophys. Res. Commun.. 260. 527-533 (1999)
[Publications] Michikawa, T. et al.: "Calmodulin mediates calcium-dependent inactivation of the cerebellar"Neuron. 23. 799-808 (1999)
[Publications] Hirota, J. et al.: "Calmodulin inhibits inositol 1,4,5-triphosphate-induced calcium"FEBS Letters. 456. 322-326 (1999)
[Publications] Nagata, E. et al.: "Selective inhibition of 1,4,5-triphosphate-induced Ca2+"Neuroscience. 93. 995-1001 (1999)
[Publications] Mitsuyama, F. et al.: "Microinjection of Ca2+ store-enriched microsome fractions to diving"Developmental Biology. 214. 160-167 (1999)
[Publications] Hayashi, M. et al.: "Intracellular calsium concentration in the inositol triphosphate"J Am Soc Nephrol. 10. 2094-2101 (1999)
[Publications] Boulay, G. et al.: "Modulation of Ca2+ entry by polypeptides of the IP3...."Proc. Natle. Acad. Sci. USA. 96. 14955-14960 (1999)
[Publications] Hirota, J. et al.: "Inositol 1,4,5-trisphosphate type 1 is a substate for caspase-3"J. Biol. Chem.. 274. 34430-34437 (1999)
[Publications] Baylis, H. A., et al.: "Inositol 1,4,5-trisphosphate receptors are strongly expressed"J. Mol. Biol.. 294. 467-476 (1999)
[Publications] Sugiyama, T. et al.: "Inositiol 1,4,5-trisphosphate receptors associated with focal"FEBS Letters. 466. 29-34 (2000)

1999 Fiscal Year Annual Research Report

神経可塑性と脳の発生・分化におけるIP_3受容体/Ca^<2+>シグナリングの役割

Principal Investigator

御子柴 克彦 東京大学, 医科学研究所, 教授 (30051840)

Research Products

[Publications] Yoshikawa, F. et al.: "Cooperative formation of the ligand-binding site of the inositol"J. Biol. Chem.. 274. 328-334 (1999)

[Publications] Yoshikawa, F. et al.: "Trypsinized cerebellar inositol 1,4,5-trisphosphate receptor"J. Biol. Chem.. 274. 316-327 (1999)

[Publications] Zhu. C. et al.: "Inosiotl 1,4,5-trisphosphate receptor down-regulation is activated"J. Biol. Chem.. 274. 3476-3489 (1999)

[Publications] Yoshikawa, F. et al.: "High efficient expression of the functional ligand binding site of the"Biochem. Biophys. Res. Commun.. 257. 792-797 (1999)

[Publications] Konishi. Y et al.: "Transcriptional regulation of mouse type 1 inositol 1,4,5-trisphosphate"J. Neurochem.. 72. 1717-1724 (1999)

[Publications] Ohkawa, N. et al.: "Activation of the mouse inositol 1,4,5-triphosphate receptor type 1"Gene. 229. 11-19 (1999)

[Publications] Hamada, T. et al.: "The role of inositol triphosphate-induced Ca2+ release from IP3-"Neurosience. Letters. 263. 125-128 (1999)

[Publications] Natsume, T. et al.: "Real time analysis of interaction between inositol 1,4,5-triphosphate"Biochem. Biophys. Res. Commun.. 260. 527-533 (1999)

[Publications] Michikawa, T. et al.: "Calmodulin mediates calcium-dependent inactivation of the cerebellar"Neuron. 23. 799-808 (1999)

[Publications] Hirota, J. et al.: "Calmodulin inhibits inositol 1,4,5-triphosphate-induced calcium"FEBS Letters. 456. 322-326 (1999)

[Publications] Nagata, E. et al.: "Selective inhibition of 1,4,5-triphosphate-induced Ca2+"Neuroscience. 93. 995-1001 (1999)

[Publications] Mitsuyama, F. et al.: "Microinjection of Ca2+ store-enriched microsome fractions to diving"Developmental Biology. 214. 160-167 (1999)

[Publications] Hayashi, M. et al.: "Intracellular calsium concentration in the inositol triphosphate"J Am Soc Nephrol. 10. 2094-2101 (1999)

[Publications] Boulay, G. et al.: "Modulation of Ca2+ entry by polypeptides of the IP3...."Proc. Natle. Acad. Sci. USA. 96. 14955-14960 (1999)

[Publications] Hirota, J. et al.: "Inositol 1,4,5-trisphosphate type 1 is a substate for caspase-3"J. Biol. Chem.. 274. 34430-34437 (1999)

[Publications] Baylis, H. A., et al.: "Inositol 1,4,5-trisphosphate receptors are strongly expressed"J. Mol. Biol.. 294. 467-476 (1999)

[Publications] Sugiyama, T. et al.: "Inositiol 1,4,5-trisphosphate receptors associated with focal"FEBS Letters. 466. 29-34 (2000)

御子柴克彦東京大学, 医科学研究所, 教授 (30051840)