2000 Fiscal Year Final Research Report Summary
Role of IP_3 receptor/Ca^<2+> signaling in neural plasticity and brain development
| Project/Area Number |
11308032
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| Research Category |
Grant-in-Aid for Scientific Research (A).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MIKOSHIBA Katsuhiko The Institute of Medical Science Department of Basic Medical Sciences The University of Tokyo. Professor., 医科学研究所, 教授 (30051840)
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| Project Period (FY) |
1999 – 2000
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| Keywords | IP_3 / Ca^<2+> / endoplasmic reticulum |
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| Research Abstract |
We have found that wave and Ca2+ oscillation is produced through the Ca2+ channel localized on the endoplasmic reticulum which is a Ca2+ store site inside the cell, and not through Ca2+ channel on the plasma membrane.
We used CALI(chromophore assisted laser inactivation)method using laser beam to study if IP3 receptor mediated Ca2+ movement is involved in growth cone formation and neurite extention. We used malakite green to label specific antibody against type 1 IP3 receptor. We added laser beam to the dye to produce redicals.
While control antibody showed no block in neurite extention, antibody against type IP3 showed retraction of neurite, only when laser is added at growth cone.
This suggests that IP3 receptor medited Ca2+ release is involved in neurite extention.
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