2000 Fiscal Year Annual Research Report

骨組織の無観血的高分解能形態解析システムの開発と応用

Research Project

Project/Area Number	11357016
Research Institution	NIIGATA UNIVERSITY
Principal Investigator	小澤英浩新潟大学, 歯学部, 教授 (60018413)
Co-Investigator(Kenkyū-buntansha)	網塚憲生新潟大学, 歯学部, 助手 (30242431) 入江一元北海道医療大学, 歯学部, 講師 (70223352) 江尻貞一新潟大学, 歯学部, 助教授 (40160361) 小石川篤 (株)日鉄エレックス, エンジニアリング事業本部・電子応用機器事業部・X線応用機器事業センター, 部長(研究職) 菊池一夫 (株)日鉄エレックス, エンジニアリング事業本部・電子応用機器事業部・X線応用機器事業センター, 所長(研究職)
Keywords	マイクロCT / 骨組織 / 骨密度 / 3次元構造解析 / 被爆線量
Research Abstract	【X線管+検出器回転型ガントリーの詳細設計と製作、調整】平成11年度の実験において、基準画像撮影時の被爆線量は従来比1/16〜1/50に低減したが、被爆線量目標値の110μSVとは12〜35倍の乖離があった。被爆線量をさらに軽減するため高感度型検出器を再検討した結果、高解像度(640x480ピクセル→2048x2048ピクセル)高濃度階調(10ビット→12ビット)検出器(デジタルフラットパネル)の採用を試み、撮影条件と撮影時間のトレードオフのテストを実施した。なお、X線管+検出器回転型ガントリーの詳細設計については基本設計を完了した。【骨体積密度(皮質骨&海綿骨)の測定および評価】骨体積密度を求める方法として、皮質骨等価物質よりなる体積密度既知のファントムと海綿骨等価物質よりなる体積密度既知のファントムを複数組み合わせることにより、皮質骨が海綿骨CT値に与える影響を定量的に数式化することが可能となり、海綿骨部の絶対体積密度を正確に得ることができた。【3次元画像による骨構造の評価】新規開発した高分解能マイクロフォーカスX線CT装置を用い、卵巣摘出によって生じるサル顎骨の変化、ビタミンD投与したマウスの骨格の変化、Gli遺伝子欠損マウスの骨格異常などを明らかにし、日本骨代謝学会、アメリカ骨代謝学会などにおいて報告した。また魚類咽頭歯の形態観察や血管造影剤を注入した馬の浅指屈腱内の血管分布立体的観察にも高分解能マイクロフォーカスX線CT装置が非常に有効である事を明らかにした。

Research Products
(34 results)

All Other

All Publications (34 results)

[Publications] Sasaki,T.: "Localization of alkaline phosphatase and osteopontin during matrix-mineralization, in the developing cartilage of coccygeal vertebrae."Arch.Histol.Cytol.. 63・3. 271-284 (2000)
[Publications] Amizuka,N.: "Inefficient function of the signal sequence of PTHrP for targeting into the secretory pathway."Biochemical and Biophysical Research Communications. 273・2. 621-629 (2000)
[Publications] Hoshi,K.: "Matrix vesicle calcification in bones of adult rats."Calcif.Tissue Int.. 66・3. 430-434 (2000)
[Publications] Tanizawa,T.: "Early reduction in bone formation and elevated bone resorption in ovariectomized rats with special reference to acute inflammation."Bone. 26・1. 43-53 (2000)
[Publications] Irie,K.: "The histochemical and cytochemical changes from formative to resorptive osteocytes."Acta histochem.Cytochem.. 33・5. 385-391 (2000)
[Publications] Tanaka,M.: "Region-specific bone mass changes in rat mandibular condyle following ovariectomy."J.Dent.Res.. 79・11. 1907-1913 (2000)
[Publications] Amizuak,N.: "Recent studies on the biological action of parathyroid hormone (PTH)-related peptide (PTHrP) and PTH/PTHrP receptor in cartilage and bone."Histol Histopathol. 15・2. 957-970 (2000)
[Publications] Hoshi,K.: "Ultrastructural,cytochemical,and biophysical aspects of mechanisms of bone matrix calcification."Acta Anat Nippon. 75・5. 457-465 (2000)
[Publications] Amizuka,N.: "The biological action of parathyroid hormone-relatyed peptide (PTHrP) and fibroblast growth factor receptor 3 (FGFR3) on bone and cartilage."Acta Anat Nippon. 75・5. 415-425 (2000)
[Publications] 網塚憲生: "副甲状腺ホルモン関連ペプチド遺伝子組み替えマウスの軟骨・骨の異常"医学のあゆみ. 192・6. 740-746 (2000)
[Publications] 江尻貞一: "新しい形態学的解析方法マイクロCT"日本骨代謝学会雑誌. 18・1. 15-21 (2000)
[Publications] 網塚憲生: "骨転移に関わる細胞群"ホルモンと臨床. 7・48. 11-20 (2000)
[Publications] 星和人: "骨再生療法:分子機構からみた最新知見"リサLife Support and Anesthesia. 7・8. 792-793 (2000)
[Publications] 星和人: "Carl Zeiss製エネルギーフィルター透過型電子顕微鏡"細胞. 32・7. 19-23 (2000)
[Publications] 江尻貞一: "元素分析"電子顕微鏡. 35・2. 144-145 (2000)
[Publications] 江尻貞一: "マイクロCTの原理と応用"生体の科学. 51・6. 602-607 (2000)
[Publications] Ikegame,M.: "Tensile stress induces bone morphogenetic protein 4 in preosteoblastic and fibroblastic cells, which later differentiate into osteoblasts leading to osteogenesis in the mouse calvariae in organ culture."J Bone Miner Res.. 16・1. 24-32 (2001)
[Publications] Hoshi,K.: "Localizational alterations of calcium, phosphorus, and calcification-related organics such as proteoglycans and alkaline phosphatase during bone calcification."J Bone Miner Res.. 16・2. 289-298 (2001)
[Publications] Hoshi,K.: "Histopathological characterization of melorheostosis."Orthopedics. (in press).
[Publications] Yanagisawa,H.: "Posterior interosseous nerve palsy caused by synovial chondromatosis of the elbow joint."Clin Radiol.. (in press).
[Publications] Hoshi,K.: "Ultrastructural analysis of bone calcification by using energy-filtering transmission electron microscopy."Italian Journal of Anatomy and Embryology. (in press).
[Publications] Hoshi,K.: "Observation of human dentine by focused ion beam and energy-filtering transmission electron microscopy."J Microsc. (in press).
[Publications] Ito,M.: "Bisphosphonate acts on osteoclasts independent of ruffled borders in osteosclerotic (oc/oc) mice."Bone. (in press).
[Publications] Sasakura,K.: "Osteopontin localization and expression in cellular cementum at the site root resorption during physiological tooth movement."Ortho.Waves. (in press).
[Publications] Takeyama,M.: "Immunohistochemical demonstration of cathepsins B and L in the periodontal ligament (PDL) of the rat molar."Jap.J.Oral Biol.. (in press).
[Publications] 星和人: "骨形成・再生機構の微細形態学的研究"スパインフロンティア. (印刷中).
[Publications] 星和人: "磁場と骨形成"磁気と健康、磁気健康科学研究振興財団. (印刷中).
[Publications] 網塚憲生: "アクチビン"骨粗鬆症ナビゲーター. (印刷中).
[Publications] 江尻貞一: "走査電子顕微鏡"共立出版社. 447 (2000)
[Publications] Amizuak,N.: "Recent Devel.Endocrinol."Transworld Research Network. 500 (2000)
[Publications] 網塚憲生: "カルシウムと骨「骨原生細胞」"朝倉書店(印刷中).
[Publications] 網塚憲生: "カルシウムと骨「カルシトニンの標的組織と作用」"朝倉書店(印刷中).
[Publications] 網塚憲生: "新・分子骨代謝学と骨粗鬆症"メデイカルレビュー社(印刷中).
[Publications] 小澤英浩: "新・分子骨代謝学と骨粗鬆症"メデイカルレビュー社(印刷中).

2000 Fiscal Year Annual Research Report

骨組織の無観血的高分解能形態解析システムの開発と応用

Principal Investigator

小澤 英浩 新潟大学, 歯学部, 教授 (60018413)

Research Products

[Publications] Sasaki,T.: "Localization of alkaline phosphatase and osteopontin during matrix-mineralization, in the developing cartilage of coccygeal vertebrae."Arch.Histol.Cytol.. 63・3. 271-284 (2000)

[Publications] Amizuka,N.: "Inefficient function of the signal sequence of PTHrP for targeting into the secretory pathway."Biochemical and Biophysical Research Communications. 273・2. 621-629 (2000)

[Publications] Hoshi,K.: "Matrix vesicle calcification in bones of adult rats."Calcif.Tissue Int.. 66・3. 430-434 (2000)

[Publications] Tanizawa,T.: "Early reduction in bone formation and elevated bone resorption in ovariectomized rats with special reference to acute inflammation."Bone. 26・1. 43-53 (2000)

[Publications] Irie,K.: "The histochemical and cytochemical changes from formative to resorptive osteocytes."Acta histochem.Cytochem.. 33・5. 385-391 (2000)

[Publications] Tanaka,M.: "Region-specific bone mass changes in rat mandibular condyle following ovariectomy."J.Dent.Res.. 79・11. 1907-1913 (2000)

[Publications] Amizuak,N.: "Recent studies on the biological action of parathyroid hormone (PTH)-related peptide (PTHrP) and PTH/PTHrP receptor in cartilage and bone."Histol Histopathol. 15・2. 957-970 (2000)

[Publications] Hoshi,K.: "Ultrastructural,cytochemical,and biophysical aspects of mechanisms of bone matrix calcification."Acta Anat Nippon. 75・5. 457-465 (2000)

[Publications] Amizuka,N.: "The biological action of parathyroid hormone-relatyed peptide (PTHrP) and fibroblast growth factor receptor 3 (FGFR3) on bone and cartilage."Acta Anat Nippon. 75・5. 415-425 (2000)

[Publications] 網塚憲生: "副甲状腺ホルモン関連ペプチド遺伝子組み替えマウスの軟骨・骨の異常"医学のあゆみ. 192・6. 740-746 (2000)

[Publications] 江尻貞一: "新しい形態学的解析方法マイクロCT"日本骨代謝学会雑誌. 18・1. 15-21 (2000)

[Publications] 網塚憲生: "骨転移に関わる細胞群"ホルモンと臨床. 7・48. 11-20 (2000)

[Publications] 星和人: "骨再生療法:分子機構からみた最新知見"リサLife Support and Anesthesia. 7・8. 792-793 (2000)

[Publications] 星和人: "Carl Zeiss製エネルギーフィルター透過型電子顕微鏡"細胞. 32・7. 19-23 (2000)

[Publications] 江尻貞一: "元素分析"電子顕微鏡. 35・2. 144-145 (2000)

[Publications] 江尻貞一: "マイクロCTの原理と応用"生体の科学. 51・6. 602-607 (2000)

[Publications] Ikegame,M.: "Tensile stress induces bone morphogenetic protein 4 in preosteoblastic and fibroblastic cells, which later differentiate into osteoblasts leading to osteogenesis in the mouse calvariae in organ culture."J Bone Miner Res.. 16・1. 24-32 (2001)

[Publications] Hoshi,K.: "Localizational alterations of calcium, phosphorus, and calcification-related organics such as proteoglycans and alkaline phosphatase during bone calcification."J Bone Miner Res.. 16・2. 289-298 (2001)

[Publications] Hoshi,K.: "Histopathological characterization of melorheostosis."Orthopedics. (in press).

[Publications] Yanagisawa,H.: "Posterior interosseous nerve palsy caused by synovial chondromatosis of the elbow joint."Clin Radiol.. (in press).

[Publications] Hoshi,K.: "Ultrastructural analysis of bone calcification by using energy-filtering transmission electron microscopy."Italian Journal of Anatomy and Embryology. (in press).

[Publications] Hoshi,K.: "Observation of human dentine by focused ion beam and energy-filtering transmission electron microscopy."J Microsc. (in press).

[Publications] Ito,M.: "Bisphosphonate acts on osteoclasts independent of ruffled borders in osteosclerotic (oc/oc) mice."Bone. (in press).

[Publications] Sasakura,K.: "Osteopontin localization and expression in cellular cementum at the site root resorption during physiological tooth movement."Ortho.Waves. (in press).

[Publications] Takeyama,M.: "Immunohistochemical demonstration of cathepsins B and L in the periodontal ligament (PDL) of the rat molar."Jap.J.Oral Biol.. (in press).

[Publications] 星和人: "骨形成・再生機構の微細形態学的研究"スパインフロンティア. (印刷中).

[Publications] 星和人: "磁場と骨形成"磁気と健康、磁気健康科学研究振興財団. (印刷中).

[Publications] 網塚憲生: "アクチビン"骨粗鬆症ナビゲーター. (印刷中).

[Publications] 江尻貞一: "走査電子顕微鏡"共立出版社. 447 (2000)

[Publications] Amizuak,N.: "Recent Devel.Endocrinol."Transworld Research Network. 500 (2000)

[Publications] 網塚憲生: "カルシウムと骨「骨原生細胞」"朝倉書店(印刷中).

[Publications] 網塚憲生: "カルシウムと骨「カルシトニンの標的組織と作用」"朝倉書店(印刷中).

[Publications] 網塚憲生: "新・分子骨代謝学と骨粗鬆症"メデイカルレビュー社(印刷中).

[Publications] 小澤英浩: "新・分子骨代謝学と骨粗鬆症"メデイカルレビュー社(印刷中).

小澤英浩新潟大学, 歯学部, 教授 (60018413)