2001 Fiscal Year Annual Research Report

高齢化社会における口腔の健康維持に関する基礎的研究

Research Project

Project/Area Number	11357017
Research Institution	Osaka University
Principal Investigator	森本俊文大阪大学, 大学院・歯学研究科, 教授 (20028731)
Co-Investigator(Kenkyū-buntansha)	井上富雄昭和大学, 歯学部, 教授 (70184760) 岩田幸一大阪大学, 大学院・歯学研究科, 助教授 (60160115) 神田健郎東京都老人総合研究所, 生理, 主任研究員 (50009635) 山田好秋新潟大学, 大学院・医歯学総合研究科, 教授 (80115089) 増田裕次大阪大学, 大学院・歯学研究科, 講師 (20190366)
Keywords	咀嚼 / 軟食 / 疼痛 / CFA / c-Fos / 炎症 / ラット
Research Abstract	本研究は、咀嚼、嚥下等の口腔機能の加齢に伴う変化が、いかなる中枢神経系の変化によってもたらされるかを知ることにより、口腔の健康維持に役立てようとするものである。また、加齢に伴う口腔機能の低下が全身にもたらす影響を調べることにより、口腔の健康の重要性を明らかにしようとするものである。本年度は、後者について研究を進めた。加齢に伴い口腔あるいは全身的な疾患により、軟らかい食品しか摂食しえない場合を想定し、実験的に軟食を続けた場合と軟食を続けてから、硬い食品を与えた場合に痛みに対する感受性に変化が認められるかどうかについて調べた。雄性SD系ラットを用い、以下の2群に分けて、実験を行なった。ひとつは軟食群で、軟食を10日間与え、起炎物質であるCFAを足底皮下に注入し、その後も軟食を与え続けて3日後に灌流した群、もうひとつは硬食群で軟食を10日間与えCFAを足底皮下に注入し、1日後から硬食を与え3日後に灌流した群である。熱刺激に対する逃避潜時からCFAを足底皮下に注入後にみられる疼痛閾値低下は、軟食群に比べ硬食群で有意に抑制された。L4、5の脊髄後角部に発現する疼痛情報の指標であるc-Fos陽性細胞は軟食群で注入側に多数認められた。これに対し、硬食群では少数認められたのみであった。また、ナロキソンの持続注入により硬食群で観察されたc-Fos陽性細胞発現の抑制は消失した。以上のことから、軟食摂取から硬食摂取に変えた時にみられる疼痛抑制は、オピオイドを介して発現する可能性が示された。

Research Products

(18 results)

All Other

All Publications (18 results)

[Publications] Masuda, Y., et al.: "Neuronal activity in the putamen and the globus pallidus of rabbit during mastication"Neurosci.Res.. 39. 11-19 (2001)
[Publications] Komuro, A., et al.: "Influence of food thickness and hardness on possible feed-forward control of the masseteric muscle activity the anesthetized rabbit"Neurosci.Res.. 39. 21-29 (2001)
[Publications] Komuro, A., et al.: "Putative feed-forward control of jaw-closing muscle activity during rhythmic jaw movements in the anesthetized rabbit"J.Neurophysiol.. 86. 2834-2844 (2001)
[Publications] Iwata, K., et al.: "Alteration of medullary dorsal horn neuronal activity following inferior alveolar nerve transection in rats"J.Neurophysiol.. 86. 2868-2877 (2001)
[Publications] Kobayashi, M., et al.: "Electrophysiological analysis of rhythmic jaw movements in the freely-moving mouse"Physiol.Behav.. 75. 1-9 (2001)
[Publications] Dai, Y., et al.: "A selective increase in Fos expression in the spinal dorsal horn neurons following quantitative thermal stimulation in rats with experimental mononeuropathy"Pain. 90. 287-296 (2001)
[Publications] Kanda, K., et al.: "Temporal facilitation of the flexor reflex : Comparison between young and aged rats"Neurosci.Lett.. 304. 49-52 (2001)
[Publications] Imbe, H., et al.: "Orofacial deep and cutaneous tissue inflammation and trigeminal activation"Cells Tissue Org.. 169. 238-247 (2001)
[Publications] Koga, Y., et al.: "Development of a three-dimensional jaw-tracking system implanted in the freely moving mouse"Med.Engineering Physics.. 23. 201-205 (2001)
[Publications] Inoue, M., et al.: "Changes in jaw reflexes by stimulation of the hypothalamus in anesthetized rabbit"Neurosci.Res.. 41. 61-65 (2001)
[Publications] Naganuma, K., et al.: "Tongue and jaw muscle activities during chewing and swallowing in freely behaving rabbits"Brain Res.. 915. 185-194 (2001)
[Publications] Takagi, M., et al.: "Analysis of swallowing movement using a simple and safe device"J.Jpn.Soc.Stomatognath.Funct.. 8. 25-30 (2002)
[Publications] Iwata, K., et al.: "Plastic changes in nociceptive transmission of the rat spinal cord with advancing age"J.Neurophysiol.. 87. 1086-1093 (2002)
[Publications] Kondo, E., et al.: "Involvement of glutamate receptors on hyperexcitability of wide dynamic range neurons in the gracile nucleus of the rats with experimental mononeuropathy"Pain. 95. 153-163 (2002)
[Publications] Nomura, H., et al.: "Induction of Fos protein-like immunoreactivity in the trigeminal spinal nucleus caudalis and upper cervical cord following noxious and non-noxious mechanical stimulation of the whisker pad of the rat with an inferior alveolar nerve transection"Pain. 95. 225-238 (2002)
[Publications] Kakizaki, Y., et al.: "Coordination between the masticatory and tongue muscles as seen with different foods in consistency and in reflex activities during natural chewing"Brain Res.. 929. 210-217 (2002)
[Publications] 山田好秋(分担): "嚥下リハビリテーションと口腔ケア"メヂカルフレンド社. 232 (2001)
[Publications] 山田好秋(分担): "摂取・嚥下障害の評価法と食事指導"医歯薬出版. 198 (2001)

2001 Fiscal Year Annual Research Report

高齢化社会における口腔の健康維持に関する基礎的研究

Principal Investigator

森本 俊文 大阪大学, 大学院・歯学研究科, 教授 (20028731)

Research Products

[Publications] Masuda, Y., et al.: "Neuronal activity in the putamen and the globus pallidus of rabbit during mastication"Neurosci.Res.. 39. 11-19 (2001)

[Publications] Komuro, A., et al.: "Influence of food thickness and hardness on possible feed-forward control of the masseteric muscle activity the anesthetized rabbit"Neurosci.Res.. 39. 21-29 (2001)

[Publications] Komuro, A., et al.: "Putative feed-forward control of jaw-closing muscle activity during rhythmic jaw movements in the anesthetized rabbit"J.Neurophysiol.. 86. 2834-2844 (2001)

[Publications] Iwata, K., et al.: "Alteration of medullary dorsal horn neuronal activity following inferior alveolar nerve transection in rats"J.Neurophysiol.. 86. 2868-2877 (2001)

[Publications] Kobayashi, M., et al.: "Electrophysiological analysis of rhythmic jaw movements in the freely-moving mouse"Physiol.Behav.. 75. 1-9 (2001)

[Publications] Dai, Y., et al.: "A selective increase in Fos expression in the spinal dorsal horn neurons following quantitative thermal stimulation in rats with experimental mononeuropathy"Pain. 90. 287-296 (2001)

[Publications] Kanda, K., et al.: "Temporal facilitation of the flexor reflex : Comparison between young and aged rats"Neurosci.Lett.. 304. 49-52 (2001)

[Publications] Imbe, H., et al.: "Orofacial deep and cutaneous tissue inflammation and trigeminal activation"Cells Tissue Org.. 169. 238-247 (2001)

[Publications] Koga, Y., et al.: "Development of a three-dimensional jaw-tracking system implanted in the freely moving mouse"Med.Engineering Physics.. 23. 201-205 (2001)

[Publications] Inoue, M., et al.: "Changes in jaw reflexes by stimulation of the hypothalamus in anesthetized rabbit"Neurosci.Res.. 41. 61-65 (2001)

[Publications] Naganuma, K., et al.: "Tongue and jaw muscle activities during chewing and swallowing in freely behaving rabbits"Brain Res.. 915. 185-194 (2001)

[Publications] Takagi, M., et al.: "Analysis of swallowing movement using a simple and safe device"J.Jpn.Soc.Stomatognath.Funct.. 8. 25-30 (2002)

[Publications] Iwata, K., et al.: "Plastic changes in nociceptive transmission of the rat spinal cord with advancing age"J.Neurophysiol.. 87. 1086-1093 (2002)

[Publications] Kondo, E., et al.: "Involvement of glutamate receptors on hyperexcitability of wide dynamic range neurons in the gracile nucleus of the rats with experimental mononeuropathy"Pain. 95. 153-163 (2002)

[Publications] Kakizaki, Y., et al.: "Coordination between the masticatory and tongue muscles as seen with different foods in consistency and in reflex activities during natural chewing"Brain Res.. 929. 210-217 (2002)

[Publications] 山田好秋(分担): "嚥下リハビリテーションと口腔ケア"メヂカルフレンド社. 232 (2001)

[Publications] 山田好秋(分担): "摂取・嚥下障害の評価法と食事指導"医歯薬出版. 198 (2001)

森本俊文大阪大学, 大学院・歯学研究科, 教授 (20028731)