2000 Fiscal Year Final Research Report Summary
Studies on the utilization of useful genes isolated from the wild plant for control of virus diseases
| Project/Area Number |
11460024
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物保護
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
OSAKI Takeshi Graduate School of Agr.Biol.Sci., Osaka Prefecture University, Prof., 農学生命科学研究科, 教授 (00081555)
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| Co-Investigator(Kenkyū-buntansha) |
OHTA Daisaku Graduate School of Agr.Biol.Sci., Osaka Prefecture University, Assist.Prof., 農学生命科学研究科, 助教授 (10305659)
MORIKAWA Toshinobu Graduate School of Agr.Biol.Sci., Osaka Prefecture University, Assist.Prof., 農学生命科学研究科, 助教授 (90145821)
OHKI Satoshi Graduate School of Agr.Biol.Sci., Osaka Prefecture University, Assist.Prof., 農学生命科学研究科, 助教授 (00128761)
FURUKAWA Hazime Graduate School of Agr.Biol.Sci., Osaka Prefecture University, Lecture, 農学生命科学研究科, 講師 (40240957)
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| Project Period (FY) |
1999 – 2000
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| Keywords | Wild plant / Cucumis figarei / Virus resistance / CF-RIP gene / Figaren / Antiviral protein / Control of virus disease / RNase |
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| Research Abstract |
A cDNA coding a putative ribosome-inactivating protein(CF-RIP) from Cucunis figarei was cloned and sequenced. The entire cDNA contains an ORF encoding a protein of 286 amino acids which share conserved residues for ribosome-inactivating proteins(RIPS). CF-RIP share 54.8-64.5% identity with four RIPs from other cucurbits. RT-PCR analysis indicated that CF-RIP mRNA was expressed in leaf and stem tissues of Cucumis figarei.
An antiviral protein, designated figaren, was purified from leaves of C.figarei and partially characterized. Column chromatography, SDS-PAGE and periodic acid-Schiff staining revealed that figaren is a glycoprotein with a molecular weight of 23 kDa. N-terminal amino acid sequencing indicated that figaren contained the conserved region for the S-allele-associated ribonucleases(RNases). In-gel RNase assay showed that figaren digested yeast RNAs. Fluorescence in situ lybridization revealed that figaren and Rnases(beef pancrease and RNase T1)at 1μ g/ml similarly inhibited C
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MV infection in cowpea leaves. Figaren and Rnases at 5-500ng/mlhad similar inhibitory effect on local lesion formation by CMV.CMV accumulation detected by ELISA in tobacco leaves treated with figaren 6 or 0 hr before inoculation with CMV was suppressed. When upper leaves of tobacco plants were treated with figaren and inoculated 10 min later with CMV, mosaic symptoms were delayed for 5-7 days on most of tobacco plants, and some plants remained asymptomatic. The amount of CMV RNAs and CMV antigen in melon protoplasts inoculated with CMV and subsequently inoculated with figaren similarly increased with time as did that the control ELISA and local lesion assays indicated that CMV infection on the upper surfaces of the leaves of tobacco, melon, cowpea and Chenopodium amaranticolor whose lower surfaces had been treated with figaren 5-10 min before CMV inoculation was almost completely inhibited. Figaren almost completely inhibited CMV infection on the untreated halves of leaves of tobacco. Figaren also induced localized acquired resistance in tobacco plants. Less
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Research Products
(14 results)
