2000 Fiscal Year Final Research Report Summary
Analysis of novel insecticidal crystal protein genes from B.thuringiensis and application of biological insecticide
| Project/Area Number |
11460026
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B).
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
蚕糸・昆虫利用学
|
|---|
| Research Institution | HOKKAIDO UNIVERSITY |
|---|
Principal Investigator |
ASANO Shin-ichiro Hokkaido Univ.Grad.School of Agr., Asso. Prof., 大学院・農学研究科, 助教授 (60222585)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SAHARA Ken Hokkaido Univ.Grad.School of Agr., Inst.., 大学院・農学研究科, 助手 (30241368)
|
|---|
| Project Period (FY) |
1999 – 2000
|
| Keywords | Bacillus thuringiensis / insecticidal crystal protein / cry gene / Scarab-cidal / Nematoda-cidal / Lepidoptera-cidal |
|---|
| Research Abstract |
1. Screening of a scarab-cidal B.thuringiensis (1999)
In order to find of a scarab-cidal B.thuringiensis, we were screening our B.thuringiensis stocks.
2. Analysis of the scarab-cidal B.thuringiensis strain (gallariae BBT2-8)(2000)
We found the scarab-cidal Bt.BBT2-8, then we cloned the crystal protein genes (cryB28 gene) have a scarab-cidal activity. This cryB28 gene was expressed in E.coli and acrystariferous B.thuringiensis strain (BT51). The expression products had a highly activity against scarabe.
3. Screening of a nematoda-cidal B.thuringiensis (1999-2000)
In order to find of a nematoda-cidal B.thuringiensis, we were screening our B.thuringiensis stocks by using PCR.We could not find nematodacidal B.thuringiensis strains.
4. Screening of novel lepidopteran-cidal B.thuringiensis (1999)
In order to find of novel lepidopteran-cidal B.thuringiensis, we were screening our B.thuringiensis stocks. We picked up the novel lepidopteran acitive Bt strain (wuhanensis), then we cloned the novel cry1 gene (cry1Db1) from wuhanensis have insecticidal activities against Spodoptera litura, Plutella xylostella and Bombyx mori.
|
