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2001 Fiscal Year Final Research Report Summary

Community structure Of biota in paddy field ecosystems

Research Project

Project/Area Number 11460031
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionNagoya University

Principal Investigator

KIMURA Makoto  Nagoya University, Graduate School of Bioagricultural Sciences, Professor, 大学院・生命農学研究科, 教授 (20092190)

Co-Investigator(Kenkyū-buntansha) MURASE Jun  Nagoya University, Graduate School of Bioagricultural Sciences, Assistant Professor, 大学院・生命農学研究科, 助手 (30285241)
ASAKAWA Susumu  Nagoya University, Graduate School of Bioagricultural Sciences, Associate Professor, 大学院・生命農学研究科, 助教授 (50335014)
Project Period (FY) 1999 – 2001
Keywordsaquatic organism / flood water / phospholipid fatty acid / community structure / PCR-DGGE / PCR-RFLP / rice rhizosphere / Rice straw
Research Abstract

The planktonic community in floodwater (FW) was investigated by collecting aquatic organisms periodically all the year round. The number of varieties (at the order level) was from 33 to 38 in the period of rice cultivation, and Lemanaceae, Pharyngophorida and Turbellaria were important members in the seasonal variation after midseason drainage. Thirty six varieties of aquatic organisms were found during the off-crop season under drained conditions. Different kinds of aquatic organisms survived at different habitats, but in general the soil beneath rice stubble was the main site for survival of aquatic organisms in the paddy field.
The bacterial communities in FW, plow, layer (PL), and percolating water (PW) were compared for the phospholipid fatty acid (PLFA) profile. Straight, mono-unsaturated PLFAs (biomarker of Gram-negative bacteria) accounted for a large proportion in FW and PW, while branched-chain PLFAs (biomarker of Gram-positive bacteria) took a large proportion in PL. Branched-chain PLFAs accounted for a large proportion in RS in submerged decomposition. PCR-RFLP pattern analysis indicated that the bacterial succession occurred in RS as RS decomposed and in plant residue (size more than 0.5mm) as the size of plant residue became finer.
The bacterial community of rice roots was studied for the PCR-RFLP and PCR-DGGE patterns after separating roots according to the nodal number. Cluster analysis showed that the difference in the community structure of respective nodal roots was more significant among the growth stage than among nodal roots with different nodal number. Microbial succession also seemed to occur as the respective nodal roots aged.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Guang-Hua Wang: "Effect of fungicide Thiram on microbial communities of rice seed surface estimated by culture-dependent and culture-independent (PCR-RFLP) methods"Soil Science and Plant Nutrition. 47・2. 303-314 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masatsugu Yamazaki: "Spatial distribution of aquatic organisms in a paddy field during off-crop season under drained conditions"Edaphologia. 68. 23-31 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Makoto Kimura: "Phospholipid fatty acid composition of microbiota in the percolating water from a rice Paddy microcosm"Soil Science and Plant Nutrition. 47・3. 533-545 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Makoto Kimura: "Microbiota responsible for the decomposition of rice straw in a submerged paddy soil estimated from phospholipid fatty acid composition"Soil Science and Plant Nutrition. 47・3. 569-578 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masatsugu Yamazaki: "Aquatic organisms in the floodwater of a Japanese paddy field under a long-term fertilizer trial"Soil Science and Plant Nutrition. 47・3. 587-599 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ainin Niswati: "Microbiota associated with microcrustacean in the floodwater of a Japanese paddy field estimated by RFLP analysis"Soil Science and Plant Nutrition. 48・1. (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Cho Cho Tun and Makoto Kimura: "Microscopic observation of the decomposition process of leaf blade of rice straw and colonizing microorganisms in a Japanese paddy field soil during the cultivation period of paddy rice"Soil Science and Plant Nutrition. Vol. 46, No. 1. 127-137 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akiko Okabe, Koki Toyota and Makoto Kimura: "Seasonal variations of phospholipid fatty acid Composition in the floodwater of a Japanese paddy under a long-term fertilizer trial"Soil Science and Plant Nutrition. Vol. 46, No. 1. 177-188 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshiteru Muraoka, Eitaro Hamakawa. Koki Toyota and Makoto Kimura: "Observation of microbial colonization on the surface of rice roots along with their development and degradation"Soil Science and Plant Nutrition. Vol. 46, No. 2. 491-502 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akiko Okabe, Hiroe Oike, Koki Toyota and Makoto Kimura: "Comparison of phospholipid fatty acid composition in floodwater and plow layer soil during the rice cultivation period in a Japanese paddy field"Soil Science and Plant Nutrition. Vol. 46, No. 4. 893-904 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Guang-Hua Wane. Yasunori Nakajima, Ichiro Shaku and Makoto Kimura: "Effect of fungicide Thiram on microbial communities of rice seed surface estimated by culture-dependent and culture-independent (PCR-RFLP) methods"Vol. 47, No. 3. 303-314 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masatsugu Yamazaki. Yukihiro Hamada. Nobuchika Kamimoto, Takashi Momii and Makoto Kimura: "Spatial distribution of aquatic organisms in a paddy field during off-crop season under drained conditions"Edaphologia. Vol. 68. 23-31 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Makoto Kimura, Hiroyoshi Kishi. Akiko Okabe and Nagamitsu Maie: "Phospholipid fatty acid composition of microbiota in the percolating water from a rice paddy microcosm"Soil Science and Plant Nutrition. Vol. 47, No.3. 533-545 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Makoto Kimura, Mariko Miyaki, Ko-Ichiro Fujinaka and Nagamitsu Male/: "Microbiota responsible for the decomnosition of rice straw in a submerged paddy soil estimated from phospholipid fatty acid composition"Soil Science and Plant Nutrition. Vol. 47, No.3. 569-578 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masatsugu Yamazaki, Yukihiro Hamada, Takeo Ibuka, Takashi Momii and Makoto Kimura: "Aquatic organisms in the floodwater of a Japanese paddy field under a long-term fertilizer trial"Soil Science and Plant Nutrition. Vol. 47, No.3. 587-599 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ainin Niswati, Makoto Ikenaga and Makoto Kimura: "Microbiota associated with microcrustacean in the floodwater of a Japanese paddy field estimated by RFLP analysis"Soil Science and Plant Nutrition. Vol. 48, No.2(in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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