| Research Abstract |
I cloned the glucodextranase gene, and found that showed the similarity to some of glucoamylase, it is possible to determine the region for bind to α-1,4 orα-1,6 glucosidic linkages. Also the isomaltotrio-dextranase gene was isolated and expressed in E. coll. The expressed enzyme was analyzed.
Starch branching enzyme (BE) is concern to biosynthesis of starch, which catalyze the hydrolysis ofα-1,4-glucosidic linkage and transferring it to make α-1,6 point. I cloned two types of this enzyme, and succcessed to express them in E, coll. Chimeric enzymes from these isozymes were analyzed. N-terminal was important to determine the affinity to amylose or amylopectin. And some chimeric enzymes which have different properties were made and analyzed.
|
