2001 Fiscal Year Final Research Report Summary
Studies on glycosidases : Recognition of substrate and alteration of function
Project/Area Number |
11460032
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
応用微生物学・応用生物化学
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Research Institution | Hokkaido University |
Principal Investigator |
MATUI Hirokazu Hokkaido Univ., Grad. school of Agriculture. Prof., 大学院・農学研究科, 教授 (90109504)
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Project Period (FY) |
1999 – 2001
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Keywords | Glycosidase / Glucodextranase / Isomaltotrio-dextranase / Starch branching enzyme / Chimeric enzymes / Substnate Specificity |
Research Abstract |
I cloned the glucodextranase gene, and found that showed the similarity to some of glucoamylase, it is possible to determine the region for bind to α-1,4 orα-1,6 glucosidic linkages. Also the isomaltotrio-dextranase gene was isolated and expressed in E. coll. The expressed enzyme was analyzed. Starch branching enzyme (BE) is concern to biosynthesis of starch, which catalyze the hydrolysis ofα-1,4-glucosidic linkage and transferring it to make α-1,6 point. I cloned two types of this enzyme, and succcessed to express them in E, coll. Chimeric enzymes from these isozymes were analyzed. N-terminal was important to determine the affinity to amylose or amylopectin. And some chimeric enzymes which have different properties were made and analyzed.
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Research Products
(13 results)
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[Publications] S. Hamada, K. Nozaki, H. Ito, Y, Yoshimoto, H. Yoshida, S. Hiraga, S. Onodera, M. Honma, Y. Takeda, H. Matsui: "Two starch branching enzymes isoforms occur in different fractions of developing seeds of kidney bean"Biochem. J.. 359. 23-34 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] J.-H. Lee, M. Tsuji, M. Nakamura, M. Nishimoto, M. Okuyama, H. Mori, A. Kimura, H. Matsui, S. Chiba: "Purification and identification of the essential ionizable groups of honeybee, Apis mellifera L, trehalase"Biosci. Biotech. Biochem.. 65. 2657-2665 (2001)
Description
「研究成果報告書概要(欧文)」より