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2002 Fiscal Year Final Research Report Summary

Plant xenobiotic-metabolizing enzyme genes for phytoremeditation of environmental endocrine disruptors

Research Project

Project/Area Number 11460046
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionKobe University

Principal Investigator

OHKAWA Hideo  Research Center for Environmental Genomics, Professor, 遺伝子実験センター, 教授 (90233048)

Co-Investigator(Kenkyū-buntansha) IMAISHI Hiromasa  Research Center for Environmental Genomics, Associate Professor, 遺伝子実験センター, 助教授 (50223318)
Project Period (FY) 1999 – 2002
KeywordsCYP71A18 / pelargonic acid / chlorotoluron / CYP71A11 / bromoxynil / CYP71R4 / herbicide cross-resistance / Lolium
Research Abstract

Contamination of the environment and agricultural products with environmental endocrine disruptors is a serious global problem. These chemicals were found in the environment and agricultural products at nano-level concentrations, and are suspected of affecting ecosystems and human health. Therefore, it is requested to develop novel technologies to remove contamination by nano-level concentrations of environmental endocrine disruptors, since most conventional technologies are high cost, take a long time to obtain results, and do not work effectively to remove contamination of nano-level chemicals. Certain plant species are grounding and have deep roots. These plants absorb and accumulate nano-level concentrations of environmental chemicals from a wide area through their root systems. On the other hand, it was found that certain enzymatic systems including cytochrome P450 (P450 or CYP) monooxygenases recognize and metabolize lipophilic xenobiotics. We attempted to clone genes coding for … More enzymes which metabolize environmental endocrine disruptors and to evaluate them for phytoremediation.
(1) Tobacco CYP71A11 gene was induced by the treatment with 2,4-D, bromoxynil and others to produce the CYP71A11 enzyme, which catalyzed N-demethylation of the herbicide chlorotoluron. Thus, CYP71A11 enzyme seemed to be important for the herbicide selectivity and resistance. Also, we have identified bromoxynil-response cis element in the promoter region of the CYP71A11 gene.
(2) Rice CYP72A18 cDNA was cloned and expressed in yeast. Yeast microsomes containing CYP72A11 enzyme metabolized the herbicide pelargonic acid. Thus, CYP72A11 was found to catalyze ω-1 hydroxylation of the herbicide pelargonic acid.
(3) The Lolium biotype WLR2 showing multiple herbicide cross-resistance specifically expressed CYP71R4 gene. Then, CYP71R4 cDNA was cloned and expressed in yeast. Yeast microsomes containing CYP71R4 enzyme metabolized the herbicide chlorotoluron. Thus, CYP71R4 was found to be involved in herbicide cross-resistance. Less

  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Y.Ohkawa, H.Ohkawa: "Transgenic Rice and Potato Plants Expressing Human Cytochrome P45Os Show Cross-Tolerance to Herbicides by Detoxifying Them"Proceedings of International Workshop Seed & Seedling Science & Technology, June 11-15, 2001, Taichung Taiwan ROC. 115-127 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大川秀郎: "環境負荷化学物質の環境モニタリング及び環境汚染浄化を目的とした遺伝子組換え植物"BRAIN. 86. 19-22 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Yamada, Y.Ohashi, M.Ohshima, H.Inui, N.Shiota, H.Ohkawa, Y.Ohkawa: "Inducible cross-tolerance to herbicides in transgenic potato plants with the rat CYP1A1 gene"Theor.Appl.Genet. 104. 308-314 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Yamada, T.Ishige, N.Shiota, H.Inui, H.Obkawa, Y.Ohkawa: "Enhancement of metabolizing herbicides in young tubers of transgenic potato plants With the rat CYP1A1 gene"Theor.Appl.Genet.. 105. 515-520 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Ohkawa, H.Ohkawa: "Transgenic Rice and Potato Plants Expressing Human Cytochrome P45Os Show Cross-Resistance to Herbicides by Detoxifying them"Food & Fertilizer Technology Center, Technical Bulletin. 159. 1-10 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Ohkawa: "Strategies for Transgenic Plants Reducing Contamination of Environmental Chemicals"Farming Japan. 36-6. 22-27 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大川秀郎: "環境モニタリング・汚染浄化植物の開発"バイオサイエンスとインダストリー. 60(3). 186-188 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大川安信, 大川秀郎: "植物の機能を利用した環境の修復"化学と生物. 40(4). 239-244 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大川秀郎: "環境負荷化学物質の負荷軽減型作物"農林水産技術 研究ジャーナル. 25(4). 38-43 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 児玉 進, 乾 秀之, 大川秀郎: "チトクロームP450の分子機能と難分解性有機汚染物質のモニタリング及び負荷軽減への応用"化学と生物. (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Rene Feyereisen, Francis Durst, Kenneth A.Feldmann, David W.Galbraith, Hideo Ohkawa, Hans D.Van Etten: "Systematic Reverse Genetic of the P450 Superfamily in Arabidopsis"RG 280/1999-M HFSP. 5 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大川秀郎, 乾 秀之, 今石浩正: "第5節 農薬等負荷軽減作物、「植物代謝工学ハンドブック」"(株)エス・ティー・エス. 780 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y. Ohkawa, and H. Ohkawa: "Transgenic Rice and Potato Plants Expressing Human Cytochrome P450s Show Cross-Tolerance to Herbicides by Detoxifying Them"Proceedings of International Workshop Seed & Seedling Science & Technology, June 11-15, 2001, Taichung Taiwan ROC. 115-127 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Ohkawa: "Transgenic Plants for Monitoring and Remediation of Environmental Contaminants"BRAIN Techno News. 86. 19-22 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Yamada, Y. Ohashi, M. Ohshima, H. Inui, N. Shiota, H. Ohkawa, and Y. Ohkawa: "Inducible cross-tolerance to herbicides in transgenic potato plants with the rat CYP1A1 gene"Theor. Appl. Genet.. 104. 308-314 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Yamada, T. Ishige, N. Shiota, H. Inui, H. Ohkawa, and Y. Ohkawa: "Enhancement of metabolizing herbicides in young tubers of transgenic potato plants with the rat CYP1A1 gene"Theor. Appl. Genet.. 105. 515-520 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Ohkawa, and H. Ohkawa: "Transgenic Rice and Potato Plants Expressing Human Cytochrome P450s Show Cross-Resistance to Herbicides by Detoxifying them"Food & Fertilizer Technology Center, Technical Bulletin. 159. 1-10 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Ohkawa: "Strategies for Transgenic Plants Reducing Contamination of Environmental Chemicals"Farming Japan. 36-6. 22-27 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Ohkawa: "Development of Plants for Environmental Monitoring and Remediation"Bioscience & Industry. 60 (3). 186-188 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Ohkawa, and H. Ohkawa: "Environmental Remediation by the Use of Plant Functions"KASEAA. 40 (4). 239-244 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Ohkawa: "Phytoremediation of Environmental Contaminants"Research Journal of Food and Agriculture. 25 (4). 38-43 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Kodama, H. Inui, and H. Ohkawa: "Cytochrome P450 Pollutants and Monitoring and Remediation of Persistent Organic Pollutants"KASEAA. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Rene Feyereisen, Francis Durst, Kenneth A. Feldmann, David W. Galbraith, Hideo Ohkawa, Hans D. Van Etten: "Systematic Reverse Genetic of the P450 Superfamily in Arabidopsis"RG 280/ 1999-M HFSP. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Ohkawa, H. Inui, and H. Imaishi, Eds by A. Shinmyo, and K. Yoshida: "Crop Plants for Degrading Environmental Chemicals including Pesticides in: Handbook of Plant Metabolic Engineering"STS. Inc.. 492-503 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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