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2001 Fiscal Year Final Research Report Summary

Study of the role of phototoxins in toxin-induced symptom development

Research Project

Project/Area Number 11460052
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

OIKAWA Hideaki  Hokkaido Univ. Grad. School of Agriculture, Associate Prof., 大学院・農学研究科, 助教授 (00185175)

Co-Investigator(Kenkyū-buntansha) FURUICHI Naotaka  Niigata Univ. Fac. of Agriculture, Associate Prof., 農学部, 助教授 (50238664)
Project Period (FY) 1999 – 2001
Keywordspathogenic fungi / phytotoxins / solanapyrone / alternaric acid
Research Abstract

A number of phytopathogenic fungi produce variety of phytotoxins. The role of the phytotoxins in plant diseases, however, still needs to be clarified. Construction of genomic DNA library of causal fungus of potato early blight, Alternaria solani, and homology based PCR allowed us to identify polyketide synthase (PKS) responsible for biosynthesis of reduced polyketadie, most likely phytotoxin solanapyrone. Expression of the identifed PKS gene in Asperguillus oryzae made production of a new pyrone whose structure shows striking similarity to solanapyrone.
Physiological effects of alternaric acid (AA), a host-specific compound produced by Alternaria solani on a putative plasma membrane kinase of RiCDPK of potato and on hypersensitive cell death of tomato and potato cells were studied. We demonstrated that a fusion protein encoded by Ca^2+-dependent protein kinase from potato RiCDPK2 gene of cv. Rishiri, purified from E. coli host cells, was stimulated by 25 uM AA from A. solani. In this study, the kinase activity of fusion protein RiCDPK2 is differentially affected by the host-specific toxin (HST) AA and inhibited by solanapyrone A (non-HST), both produced by A. solani. Moreover, from the results, Ca^2+ and Mg2+ may play an important role in the interaction between the toxins and RiCDPK2-fusion protein. We suggest that the HST from A. solani may regulate RiCDPK2 kinase activity during the infection process, leading to the inhibition of HR in the compatible interaction between the host plant and A. solani, as does the suppressor of hypersensitivity from Phytophthom infestans in the host cells. The toxic compounds, which are produced by the pathogen, may help the pathogen establish successful infection by suppressing rapid hypersensitive cell death during the host-parasite interaction.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] A.Hassan: "The Role of Calcium-Dependent Protein Kinase (CDPK) of Potato Microsomal Fraction in Signal Transduction of the Hyphal Cell Wall Component and the Suppressor from Phytophthora infestans (Mont.) de Bary. Bull"Bull Facul. Agric. Niigata Univ.. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Fukui: "NMR determination of the absolute configuration of a macrophomate synthase inhibitor by using an axial chiral reagent"Biosci. Biotechnol. Biochem. 65・8. 1920-1923 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Watanabe: "Reaction Mechanism of the Macrophomate Synthase : Experimental Evidence on Intermediacy of a Bicyclic Compound"Tetrahedron Lett.. 41・9. 1443-1446 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Watanabe: "Macrophomate Synthase : Characterization, Sequence, and Expression in Escherichia coli of the Novel Enzyme"J. Biochem. 127・3. 467-473 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Oikawa: "Potent Inhibition of Macrophomate Synthase by Reaction Intermediate Analogs"Biosci. Biotechnol. Biochem. 64・11. 2368-2379 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Watanabe: "Detailed Reaction Mechanism of Macrophomate Synthase : Extraordinary Enzyme Catalyzing Five-step Transformation"J. Boil Chem.. 275・49. 38393-38401 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Ichihara: "Comprehensive Natural Products Chemistry"Elsevier. 43 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A. Hassan et al.: "The Role of Calcium-Dependent Protein Kinase (CDPK) of Potato Microsomal Fraction in Signal Transduction of the Hyphal Cell Wall Component and the Suppressor from Phytophthora infestcms (Mont.) de Bary. Bull"Bull. Facul. Agric. Niigata Univ.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Fukui et al.: "NMR determination of the absolute configuration of a macrophomate synthase inhibitor by using an axial chiral reagent"Biosci. Biotechnol. Biochem.. 65-8. 1920-1923 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Watanabe et al.: "Reaction Mechanism of the Macrophomate Synthase: Experimental Evidence on Intermediacy of a Bicyclic Compound"Tetrahedron Lett.. 41-9. 1443-1446 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Watanabe et al.: "Macrophomate Synthase: Characterization, Sequence, and Expression in Escherichia coli of the Novel Enzyme"J. Biochem.. 127-3. 467-473 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Oikawaet et al.: "Potent Inhibition of Macrophomate Synthase by Reaction Intermediate Analogs"Biosci. Biotechnol. Biochem.. 64-11. 2368-2379 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Watanabe et al.: "Detailed Reaction Mechanism of Macrophomate Synthase: Extraordinary Enzyme Catalyzing Five-step Transformation"J. Biol. Chem.. 275-49. 38393-38401 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. Ichihara, H. Oikawa (edited book): "Elsevier"Comprehensive Natural Products Chemistry. vol. 1. 367-408 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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