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2001 Fiscal Year Final Research Report Summary

Change of Cell Wall Properties in Woody Plant Cells by Low Temperature

Research Project

Project/Area Number 11460075
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 林産学
Research InstitutionHokkaido University

Principal Investigator

FUJIKAWA Seizo  Hokkaido Univ., Grad. School of Agr., Prof., 大学院・農学研究科, 教授 (50091492)

Co-Investigator(Kenkyū-buntansha) FUNADA Ryo  Hokkaido Univ, Grad. School of Agr., Asso. Prof., 大学院・農学研究科, 助教授 (20192734)
TAKEZAWA Daisuke  Hokkaido Univ. Inst. of Low Temp. Sci., Lec., 低温科学研究所, 助手 (20281834)
ARAKAWA Keita  Hokkaido Univ. Inst. of Low Temp. Sci., Lec., 低温科学研究所, 助手 (00241381)
SANO Yuzou  Hokkaido Univ. Grad. School of Agr., Asso. Prof., 大学院・農学研究科, 助手 (90226043)
Project Period (FY) 1999 – 2001
KeywordsCold adaptation / Freezing behavior / Cell wall / Deep supercooling / Extracellular freezing / Cold-induced proteins / Untifreeze proteins / Gene cloning
Research Abstract

This study concerned to cold temperature adaptation of woody plant cells with the relation to the cell wall properties. This study provided a new evidence by cryo-scanning electron microscopy that xylem parenchyma cells of all hardwood species with thick and rigid cell walls, including boreal hardwood species, responded to subfreezing temperatures by deep supercooling. The mechanism of deep supercooling in xylem parenchyma cells has been studied in previous works and hypothesized to be due to the smaller size of micro-capillaries in the cell walls. However, this study confirmed that there was no distinct change in capillary size of the cell walls between cells exhibiting extracellular freezing arid deep supercooling. This study provided a new evidence that degree of supercooling is distinctly reduced by extraction of soluble substances in xylem cell protoplasts, suggesting the accumulation of antifreeze substances, which might drastically promote deep supercooling. We are currently analyzing candidates of antifreeze proteins and cloning their genes. In addition to these studies, this study also concerned to freezing adaptation mechanisms of cortical parenchyma cells of woody plants, which had comparatively thin soft cell walls and adapted to subfreezing temperature by extracellular freezing. All these results revealed specific physiological response of woody plant cells to environmental stresses as well as suggested the possibility of industrial application of stress-induced proteins from woody plant cells.

  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] 久保田勝利: "低温馴化によるシラカンバ木部放射柔細胞の細胞壁の変化"低温生物工学会誌. 45. 39-42 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ukaji N: "Accumulation of small-heat shock protein homologues in the endoplasmic reticulum of cortical parenchyma cells in mulberry in association with seasonal cold acclimation"Plant Physiology. 120. 481-489 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山田朋義: "低温感受性植物細胞の凍結挙動"低温生物工学会誌. 45. 7-13 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagao M: "Akinete formation in Tribonema bombycinum Derbes et solier (Xanthophyceae) in relation to freezing tolerance"Journal of Plant Research. 112. 163-174 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujikawa S: "Determination of the role of cold acclimation-induced diverse changes in plant cells from the viewpoint of avoidance of freezing injury"Journal of Plant Research. 112. 237-244 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujikawa S: "Ultrastructural aspects of freezing adaptation of cells by vitrification"In (Engelmann F & Takagi H eds) Cryopreservation of Tropical Plant Germplasm, Current Research Progress and Application, JIRCAS International Agriculture Series No. 8, International Plant Genetic Resources Institute. 36-42 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ukaji N: "Isolation and characterization of endoplasmic reticulum from mulberry cortical parenchyma cells"In (Dashek W V ed), Methods in Plant Electron Microscopy and Cytochemistry, Humana press Inc, Totawa, NJ. 147-160 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujikawa S: "Cryo-scanning electron microscopic study on freezing behavior of xylem ray parenchyma cells in hardwood species"Micron. 31. 669-686 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤川清三: "樹木の耐凍性をはかる"森林科学. 30. 50 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jitsuyama Y: "Loading process of sugars into cabbage petiole and asparagus shoot apex cells by incubation with hypertonic sugar solutions"Protoplasma. 217. 217 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ukaji N: "Cold acclimation-induced WAP27 localized in endoplasmic reticulum in cortical parenchyma cells of mulberry tree was homologous to group 3 late-embryogenesis aboundant proteins"Plant Physiology. 126. 1588-1597 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤川清三: "樹木木部柔細胞の細胞壁構造特性と寒冷適応(総説)"木材学会誌. (印刷中). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kubota M., Arakawa K., Kuroda K., Fujikawa S.: "Changes of cell wall in xylem ray parenchyma cells of birch by cold acclimation"Cryobiology and Cryotechnology. 45. 39-42 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ukaji N., Kuwabara C., Takezawa D., Arakawa K., Yoshida S., Fujikawa, S.: "Accumulation of small-heat shock protein homologues in the endoplasmic reticulum of cortical parenchyma cells in mulberry in association with seasonal cold acclimation"Plant Physiology. 120. 481-489 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada T., Kuroda K., Jitsuyama Y., Fujikawa S.: "Cryobiology and Cryotechnology"45. 7-13 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagao M., Arakawa K., Takezawa D., Yoshida S., Fujikawa S.: "Akinete Formation in Tribonema bombysnum Derbes et solier (Xanthophyceae) in relation to freezing tolerance"Journal of Plant Research. 112. 163-174 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujikawa S., Kuroda K., Jitsuyama Y.: "Determination of the role of cold acclimation-induced diverse changes in plant cells from the viewpoint of avoidance of freezing injury"Journal of Plant Research. 112. 237-244 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujikawa S., Jitsuyama Y.: "Ultrastructural aspects of freezing adaptation of cells by vitrification, In (Engelmann F., Takagi H., Eds) Cryopreservation of Tropical Plant Germplasm"Current Research Progress and Application, JIRCAS International Agriculture Series No. 8, International Plant Genetic Resources Institute. 36-42 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ukaji N., Fujikawa S., Yoshida S.: "Isolation and characterization of endoplasmic reticulum from mulberry cortical parenchyma cells, In Dashek W V ed, Methods in Plant electron microscopy and cytochemistry"Humana press Inc, Totawa. NJ. 147-160 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujikawa S., Kuroda K.: "Cryo-scanning electron microscopic study on freezing behavior of xylem ray parenchyma cells in hardwood species"Micron. 31. 669-686 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujikawa S.: "Measurement of freezing tolerance in trees"Shinrinkagaku. 30. 50-50 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jitsuyama Y., Suzuki T., Harada T., Fujikawa S.: "Loading process of sugars into cabbage petiole and asparagus shoot apex cells by incubation with hypertonic sugar solutions"Protoplasma. 217. 205-216 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ukaji N., Kuwabara C., Takezawa D., Arakawa K., Fujikawa S.: "Cold acclimation-induced WAP27 localized in endoplasmic reticulum in cortical parenchyma cells of mulberry tree was homologous to group 3 late-embryogenesis abouondant proteins"Plant Physiology. 126. 1588-1597 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujikawa S.: "Structural property of cell walls in xylem parenchyma cells of trees in relation to the freezing adaptation"Mokuzai Gakkaishi. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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