• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2001 Fiscal Year Final Research Report Summary

MOLECULAR ENDOCRINOLOGICAL STUDY ON MECHANISMS OF ARTIFICIAL MATURATION IN FEMALE JAPANESE EEL

Research Project

Project/Area Number 11460084
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

YAMAUCHI Kohei  Hokkaido Univ., Grad. School of Fish. Sci., Pro., 大学院・水産科学研究所, 教授 (10109514)

Co-Investigator(Kenkyū-buntansha) ADACHI Shinji  Hokkaido Univ., Grad. School of Fish. Sci., Asso. Pro., 大学院・水産科学研究所, 助教授 (40231930)
Project Period (FY) 1999 – 2001
KeywordsJAPANESE EEL / PITUITARY / LIVER / OVARY / VITLLOGENIN / NATURAL EEL / GTH / STEROID HORMONE
Research Abstract

1) Mechanism of pituitary GTH production
Changes in mRNA levels of GTH Iβ and IIβ subunits in pituitary of Japanese eel were examined during artificial maturation. The level of Iβ dramatically decreased immediately after the start of the treatment of artificial maturation, while the level of IIβdramatically increased. Experiment of pituitary organ culture revealed that estrogen and androgen strongly supressed the expression of Iβ, while they stimulated the expression of IIβ.
2) Mechanism of ovarian sterod production
cDNAs of P450scc, P450c17, P450arom, 3β-HSD and 17β-HSD were cloned from Japanese eel ovary. The mRNA levels of ovarian P450scc, P450c17 , P450arom and 17β-HSD gradually increased during artificial maturation although the level of P450arom decreased in final maturation phase, while the level of 3β-HSD did not change.
3) Mechanism of liver vitellogenin(VTG) production
The mRNA levels of VTG1 in liver increased after mid-vitellogenic stage, while the level of VTG2 increased immediately after the start of the treatment of artificial maturation.
4) Mechanism of sexual maturation in natural eel
The mRNA levels of GTH Iβ and IIβ in pituitary and various steroidogenic enzymes in ovary were compared between the artificially maturing Japanese eel (JP) and naturally maturing New Zealand longfinned eel (LF). The level of Iβ in LF was higher than that in JP. However, the levels of others were higher in JP than those in LF.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Ikeuchi: "Specific antibodies against synthetic oligopeptides of eel GTH/TSH subunits"Fisheries Sci.. 65. 871-877 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazeto: "Molecular cloning and characterization of Japanese eel ovarian P45Oc17 (CYP 17) cDNA"Gen. Comp. Endocrinol. 118. 123-133 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ozaki: "Developmental changes in pituitary-thyroid axis, and formation of gonads in leptocephali and glass eels of Anguilla spp"Fisheries Sci.. 66. 1115-1122 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazeto: "Cloning of 17 β-hydroxysteroid dehydrogenase-l cDNAs from Japanese eel ovary"Biochem. Biophys. Res. Commun.. 176. 840-845 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kayaba: "Ultrastructure of the oocytes of the Japanese eel Anguilla japonica during artificially induced sexual maturation"Fisheries Sci.. 67. 870-879 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazeto: "20β-hydroxysteroid dehydrogenase of the Japanese eel ovary : Its cellular localization and changes in the enzymatic activity during sexual maturation"Gen. Comp. Endocrinol. 122. 109-115 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okumura: "Changes in serum vitelligenin levels and immunohistochemical localization of vitellogenin in hepatic cells during ovarian development in the Japanese eel"Fisheries Sci.. 67. 880-887 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okumura: "Changes in hepatic vitelligenin mRNA levels during oocyte development in the Japanese eel, Anguilla japonica"Gen. Comp. Endocrinol. 125. 9-16 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikeuchi: "Specific antibodies against synthetic oligopeptides of eel GTH/TSH subunits"Fisheries Sci.. 65. 871-877 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazeto: "Molecular cloning and characterization of Japanese eel ovarian P450c17 (CYP17) cDNA"Gen. Comp. Endocrinol.. 118. 123-133 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ozaki: "Developmental changes in pituitary-thyroid axis, and formation of gonads in leptocephali and glass eels of Anguilla spp."Fishereis Sci.. 66. 1115-1122 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazeto: "Cloning of 17 β-hydroxysteroid dehydrogenase-l cDNAs from Japanese eel ovary"Biochem. Biophys. Res. Commun.. 176. 840-845 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kayaba: "Ultrastructure of the oocytes of the Japanese eel Anguilla japonica during artificially induced sexual maturation"Fisheries Sci.. 67. 870-879 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazeto: "20 β-hydroxysteroid dehydrogenase of the Japanese eel ovary: Its cellular localization and changes in the enzymatic activity during sexual maturation"Gen. Comp. Endocrinol.. 122. 109-115 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okumura: "Changes in serum vitelligenin levels and immunohistochemical localization of vitellogenin in hepatic cells during ovarian development in the Japanese eel"Fisheries Sci.. 67. 880-887 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okumura: "Changes in hepatic vitelligenin mRNA levels during oocyte development in the Japanese eel, Anguilla japonica"Gen. Comp. Endocrinol.. 125. 9-16 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2003-09-17  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi