2000 Fiscal Year Final Research Report Summary
NONCULTURE ANALYSES OF MARINE MICROBIAL COMMUNITY IN THE SEDIMENTS USING 16S rRNA GENE
| Project/Area Number |
11460085
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | UNIVERSITY OF TOKYO |
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Principal Investigator |
OHWADA Kouichi OCEAN RESEARCH INSTITUTION, UNIVERSITY OF TOKYO PROFESSOR, 海洋研究所, 教授 (30013585)
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| Co-Investigator(Kenkyū-buntansha) |
WADA Minoru OCEAN RESEARCH INSTITUTE, UNIVERSITY OF TOKYO RESEARCH ASSOCIATE, 海洋研究所, 助手 (70292860)
NISHIMURA Masahiko OCEAN RESEARCH INSTITUTE, UNIVERSITY OF TOKYO RESEARCH ASSOCIATE, 海洋研究所, 助手 (10242174)
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| Project Period (FY) |
1999 – 2000
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| Keywords | biodiversity / marine sediment / nonculture analysis / 16S rDNA / microbial community |
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| Research Abstract |
To avoid the limitation of traditional culturing techniques for assessing the microbial communities in natural environments, we have successfully developed nonculture analyses of marine microbial communities in the sediments using 16S rRNA gene. Sediment samples were taken from two sampling stations in Sagami Bay, SA and SB, and one station in Tokyo Bay, TK.After DNA extraction from the samples, PCR amplification and cloning of 16S rDNA for the samples was conducted. Then the RFLP patterns were examined after digested with three four-base-specific restriction enzymes (HhaI, RsaI and HaeIII). Thirty-seven clones were selected and sequenced according to dendrograms derived from ARDRA.Sequenced clones fell into five major lineages of the domain Bacteria : the gamma, delta and epsilon Photobacteria. Gram-positive bacteria and the division Verrucomicrobia. At SA, the Verrucomicrobia and the three subclass of the Proteobacteria were found. Most clone sequences belonged to the gamma Proteobacteria. On the other hand, the high GC Gram-positive bacteria and the gamma subslass of the Proteobacteria were common at SB and TK.All clones belonging to the high-GC Gram-positive bacteria collected from SB and TK fell into the same cluster and are regarded as members of an unknown actinomycetes group.
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