2000 Fiscal Year Final Research Report Summary
Studies on the vaccine against infection of Pathogenic bacterium Edwardsiella tarda
| Project/Area Number |
11460090
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Kochi University |
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Principal Investigator |
KAWAI Kenji Kochi University, Department of Aquaculture, Professor, 農学部, 教授 (60127925)
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| Co-Investigator(Kenkyū-buntansha) |
HOSOKAWA Hidetsuyo Kochi University, Department of Aquaculture, Professor, 農学部, 教授 (40036744)
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| Project Period (FY) |
1999 – 2000
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| Keywords | Edwardsiella tarda / outer membrane protein / protective antigen / common antigen / capsule vaccine |
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| Research Abstract |
Culture conditions for the production of the antigen, a 37 kDa outer membrane protein (Omp37Et), commonly distributed among Edwardsiella tarda strains of different serotypes was investigated using representative strain EF-1, first. Immunization of eel with purified Omp37Et prepared from 2 different serotype strains EF-l and V-l protected infection with live cells of EF-l strain. Immunization of Japanese flounder with Omp37Et prepared from EF-l strain protected infection with live cells of EF-l and V-l strains. The results indicate that the Omp37Et is the common protective antigen of E.tarda against different serotypes of E.tarda. Moreover, the immunized fish showed increase in the antibody titer and phagocyte activity. Amino acid sequeuce analysis of the Omp37Et identified 14 amino acid of N-terminal side. In the comparison of different administration methods, intraperitoneal injection, immersion and oral administration, of the formalin-killed cells vaccine of E.tarda, highest protection was resulted by injection then followed with oral administration and immersion. Immune response in the serum antibody titer and phagocyte activity showed same order as in the protection. Antigenicity of the formalin-killed cell vaccine was lowered by in vitro treatment with low pH and digestive enzymes of fish. The fact indicates that encapsulatio or coating of the vaccine antigen with anti-low pH or -enzymes to protect the antigen from the effect of those factors.
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