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2002 Fiscal Year Final Research Report Summary

Studies on Biological and Neurotoxic Activity of Amyloid Beta Protein

Research Project

Project/Area Number 11460145
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionMIYAZAKI UNIVERSITY

Principal Investigator

TATEYAMA Susumu  Miyazaki University, Agriculture, Veterinary Pathology, Professor, 農学部, 教授 (90041003)

Co-Investigator(Kenkyū-buntansha) UCHIDA Kazuyuki  Miyazaki University, Agriculture, Veterinary Pathology, Assistant Professor, 農学部, 助手 (10223554)
Project Period (FY) 1999 – 2002
KeywordsAmyloid Beta Protein / Canine / Apoptosis / Substania nigra / Lewy body / Neuropathology
Research Abstract

Age-related changes in the canine substantia nigra were examined by using immunostaining for tyrosine hydroxylase (TH). Histopathologically, several age-related changes, including lipofuscin deposition, polyglucosan bodies, amorphous basophilic inclusions and esoinophilic crystal inclusions were found in aged dogs. Immunohistochemically, TH-positive neurons were well preserved in dogs examined, however, the ratio of TH-positive neurons to GFAP-positive glial cells tended to show slight decrease in aged dogs. By ssDNA immunostaining for apoptotic cells, there were no significant results. The number of ubiquitin- and NF-positive swallen neurites was increased markedly in aged dogs. Ubiquitin-immunostaining revealed a small number of basophilic and esoinophilic inclusions, although these inclusions were negative for NF. By αSN immunostaining, no neurons were immunoreactive and no basophilic or eoinophilc intracytoplasmic inclusions were revealed. These results indicate that in the substantia nigra of aged dogs the dopaminergic neurons are well preserved, but intracytoplasmic inclusions and ubiquitin-positive degenerative neurites are commonly found. All these results have been already published in J. Vet. Med. Sci.
In the cerebral cortex and hippocampus, the number of senile plaques tended to increase according to aging. However, there was no significant relationship between the number of senile plaques and SS-DNA positive neurons, indicating beta-amyloid deposits did not induce direct neuronal apoptosis. These results have still not been published.
By the support of this grant, several neurological cases had been collected, and already reported in scientific journals as case reports.

  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] K.Uchida, et al.: "Age-related histological changes in the canine substantia nigra"Journal of Veterinary Medical Science. 65(2)(In press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Suzuki et al.: "Peripheral Neuroblastoma in a Young Labrador Retriver"Journal of Veterinary Medical Science. 65(2)(In press). (2003)

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      「研究成果報告書概要(和文)」より
  • [Publications] K.Uchida, et al.: "Ganglioglioma in the thalamus of a puppy"Journal of Veterinary Medical Science. 65(1). 113-115 (2003)

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      「研究成果報告書概要(和文)」より
  • [Publications] K.Uchida, et al.: "Thymoma and multiple thymic cysts in a dog with acquired myasthenia gravis"Journal of Veterinary Medical Science. 64(7). 637-640 (2002)

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      「研究成果報告書概要(和文)」より
  • [Publications] M.Suzuki et al.: "Cerebellar myxoid meningioma in a Shih Tzu"Journal of Veterinary Medical Science. 64(2). 115-117 (2002)

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      「研究成果報告書概要(和文)」より
  • [Publications] M. Suzuki, K. Uchida, H. Taniguchi, R. Yamaguchi, S. Tateyama: "Peripheral Neuroblastoma in a Young Labrador Retriver"J. Vet. Med. Sci.. 65(2). in press (2003)

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      「研究成果報告書概要(欧文)」より
  • [Publications] K. Uchida, N. Kihara, K. Hashimoto, H. Nakayama, R. Yamaguchi, S. Tateyama: "Age-related histological changes in the canine substantia nigra"J. Vet. Med. Sci.. 65(2). in press (2003)

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      「研究成果報告書概要(欧文)」より
  • [Publications] K. Uchida, H. Nakayama, Y. Endo, C. Kai, S. Tatewaki, R. Yamaguchi, K. Doi, S. Tateyama: "Ganglioglinoma in the thalamus of a puppy"J. Vet. Med. Sci.. 65(1). 113-115 (2003)

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      「研究成果報告書概要(欧文)」より
  • [Publications] M. Suzuki, H. Nakayama, R. Otsuka, A. Yasoshima, K. Katayama, K. Uetsuka, M. Ohta, S. Matsunaga, H. Ogawa, K. Uchida, K. Doi: "Cerebellar myxoid meningioma in a Shih Tzu"J. Vet. Med. Sci.. 64(2). 115-117 (2002)

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      「研究成果報告書概要(欧文)」より
  • [Publications] K. Uchida, Y. Awamura, T. Nakamura, R. Yamaguchi, S. Tateyama: "Thymoma and multiple thymic cysts in a dog with acquired myathenia gravis"J. Vet. Med. Sci.. 64(7). 637-640 (2002)

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      「研究成果報告書概要(欧文)」より
  • [Publications] K. Katayama, K. Kuroki, K. Uchida, H. Nakayama, M. Sasaki, M. Mochizuki, R. Nishimura, N. Sasaki, K. Doi: "A case of primitive neuroectodermal tumor (PNET)"J. Vet. Med. Sci.. 63. 103-105 (2001)

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  • [Publications] K. Uchida, M. Morozumi, R. Yamaguchi, S. Tateyama: "Diffuse leptomeningeal malignat histiocytosis in the brain and spinal cord of a Tibetan Terrier"Vet. Pathol.. 38. 219-222 (2001)

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      「研究成果報告書概要(欧文)」より
  • [Publications] H. Nakayama, W. Kiatipattanasakul, S. Nakamura, K. Miyawaki, F. Kimura, K. Uchida, K. Kuroki, T. Makifuchi, Y. Yoshikawa, K. Doi: "Fractal analysis of senile plaques observed in various animal species"Neuroscience Letters. 297. 195-198 (2001)

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      「研究成果報告書概要(欧文)」より
  • [Publications] K. Miyawaki, H. Nakayama, S. Nakamura, K. Uchida, K. Doi: "Three-dimensional structures of canine senile plaques"Acta Neuropathol.. 102. 321-328 (2001)

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      「研究成果報告書概要(欧文)」より
  • [Publications] R.D. Agungpriyono, K. Uchida, H. Tabaru, R. Yamaguchi, S. Tateyama: "Subacute massive necrotizing myocarditis by canine parvovirus type-2 (CPV-2) infection woth diffuse leukoencephalomalacia in a puppy dog"Vet. Pathol.. 36(1). 77-80 (1999)

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      「研究成果報告書概要(欧文)」より
  • [Publications] K. Uchida, M. Muranaka, Y. Horii, N. Murakami, R. Yamaguchi, S. Tateyama: "Non-prulent meningoencephalomyelitis of a Pacific striped dolphin. The first evidence of morbillivirus infection in a dolphin at the Pacific Ocean around Japan"J. Vet. Med. Sci.. 61(2). 159-162 (1999)

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  • [Publications] K. Uchida, T. Hasegawa, M. Ikeda, R. Yamaguchi, S. Tateyama: "Detection of autoantibody from necroyzing encephalitis of Pug dogs (Pug dog encephalitis)"Vet. Pathol.. 36(4). 301-307 (1999)

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Published: 2004-04-14  

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