MOLECULAR AND CELLULAR STUDIES OF MOLECULAR CHANGES OF PROTEINS ASSOCIATED WITH CHROMATIN DURING CYTOKINESIS

2001 Fiscal Year Final Research Report Summary

• Project/Area Number: 11460157
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: HIROSHIMA UNIVERSITY
• Principal Investigator: IKEGAMI Susumu HIROSHIMA UNIVERSITY, FACULTY OF APPLIED BIOLIGICAL SCIENCE, PROFESSOR, 生物生産学部, 教授 (80011980)
• Project Period (FY): 1999 – 2001
• Keywords: starfish / oocyte / nucleolus / myosin / phosphorylation / contractile ring

Research Abstract

Molecular changes of proteins associated with chromatin during cytokinesis have been studied using starfish eggs and embryos. Nucleic acid-associated protein sedignated NAAP has been purified from starfish (Asterina pectinifera) oocytes and an antibody reacting with NAAP has been prepared. Immunofluorscence images of starfish oocytes show that NAAP is primarily located in the nucleolus of the germinal vesicle (nucleus) of the oocyte. The C-terminal region of NAAP has been shown to be associated with chromatin. When starfish oocytes undergo meiotic maturation NAAP is phosphorylated by cdc2 kinase at specific sites and distributed evenly in the cytoplasm at the first meiotic prometa- to anaphases during the first meiotic division. Therefore, it is concluded that phospharylation at specific Ser or Thr residues dissociates NAAP fron chromatin.

Research Products

• [Publications] Ohta, E., Kubota, N., Suzuki, M., Ogaawa, T., Ohta, S., Ikegami, S: "Micromonospolide A, a new macrolide from Micromonospora sp."Tetrahedron Lett.. 4179-4181 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ohta, E., Ohta, S., Ikegami, S.: "Ancorinoside A Mg salt from the marine sponge, Ancorina sp., which specifically inhibits blastulation of starfish embryos"Tetrahedron. 57. 4699-4703 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hioki, H., Ooi, H., Hamano, M., Mimura, Y., Yoshio, S., Kodama, M., Ohta, S., Yanai, M., Ikegami, S.: "Enantioselective total synthesis and absolute stereostructure of hippospongic acid A"Tetrahedron. 57. 1235-1246 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sugino, H., Terakawa, Y., Yamasaki, Y., Nakamura, K., Higuchi, Y., Matsubara, M., Kuniyoshi, K., Ikegami, S.: "Molecular characterization of a novel nuclear transglutaminase which is expressed during starfish embryogenesis"Eur.J.Biochem.. 269. 1-11 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Murata-Hor M., Hfumoto, K., Fukata, Y., Kikuchi, A., Tatsuka, M., Hisoya, H.: "Myoshin II regulatory light chain as a novel substrate for AIM-1, an Auror/Ipllp-related kinase from rat"J.Biochem.. 128. 903-907 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Komaba, S., Hamano, H., Murata-Hori, M., Hosoya, H.: "Identification of myosin II kinase from sea urchin eggs as protein kinase CK2"Gene. 275. 141-148 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ohta, E. et al.: "Micromonospolide A, a new macrolide from Micromonospora sp"Tetrahedron Lett.. 42. 4179-4181 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ohta, E. et al.: "Ancorinoside A Mg salt from the marine sponge, Ancorina sp., which specifically inhibits blastulation of starfish embryos"Tetrahedron. 57. 5699-4703 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hioki, H. et al.: "Enantioselective total synthesis and absolute stereostructure of hippospongic acid A"Tetrahedron. 57. 1235-1246 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sugino, H. et al.: "Molecular characterization of a novel nuclear transglutaminase which is expressed during starfish embryogenesis"Eur. J. Biochem.. 269. 1-11 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Murata-Hori, M. et al.: "Myoshin II regulatory light chain as a novel substrate for AIM-1, an Auror/Ipl1p-related kinase from rat"J. Biochem.. 128. 903-907 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Komada, S. et al.: "Identification of myosin II kinase from sea urchin eggs as protein kinase CK2"Gene. 275. 141-148 (2001)
  • Description: 「研究成果報告書概要(欧文)」より