2000 Fiscal Year Final Research Report Summary
The role of Pax6 gene in vertebrate neural development
| Project/Area Number |
11470002
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Tohoku University |
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Principal Investigator |
OSUMI Noriko Development Neurobiology, Tohoku University, Professor, 大学院・医学系研究科, 教授 (00220343)
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| Co-Investigator(Kenkyū-buntansha) |
WAKAMATSU Yoshio Developmental Neurobiology, Tohoku University, Lecturer, 大学院・医学系研究科, 講師 (60311560)
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| Project Period (FY) |
1999 – 2000
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| Keywords | Neural development / Pax6 / Telencephalon / Boundary formation / Cortex / Striatum / Cell adhesion / Cadherins |
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| Research Abstract |
During development of the brain, numerous neurons of various different types are generated and delivered at their right positions, and the axons project their own targets to make correct neuronal networks. Recent advance in developmental neurobiology has revealed that such neuronal localization and axonal projection are based on the brain patterning. Pax6 is a member of the vertebrate Pax gene family, which encode transcriptional factors. Pax6 is expressed in discrete domains in the developing forebrain, hindbrain, and spinal cord. This research project aimed to elucidate roles of Pax6 in patterning the brain at cellular and molecular levels. We obtained the following results during the period supported by this grant.
1) We performed tracing of neuroepithelial cells labeled with Dil under mouse whole embryo culture, and elucidated when and how the boundary between the cortex and striatum is formed at the cellular level.
2) In situ hybridization revealed exclusive expression patterns of the two members of cadherin cell adhesion molecules, Rcadherin and cadherin6, in the cortex and striatum, respectively.
3) Electroporation of exogenous genes into the cultured mouse embryos demonstrated that exclusive expression patterns these cadherins play important roles in maintaining the cortex/striatum boundary.
4) Tracing neuroepithelial cells in the cadherin6 knockout mice revealed that the boundary between the cortex and striatum was formed but that movement of the striatal neuroepithelial cells increased.
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Research Products
(19 results)
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[Publications] Suzuki,R.,Shintani,T.,Sakuta,H.,Kato,A.,Ohkawara,T.,Osumi,N.,. et al.: "Identification of RALDH-3, a novel retinaldehyde dehydrogenase, expressed in the ventral region of the retina."Mechanisms of Development. 98. 37-50 (2000)
Description
「研究成果報告書概要(和文)」より
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[Publications] Nagase,T.,Shimoda,Y.,Sanai,Y.,Nakamura,S.,Harii,K.,Osumi,N.: "Differential Expression of Two Glucuronyltransferases Synthesizing HNK-1 carbohydrate epitope in the sublineages of the rat myogenic progenitors."Mechanisms of Development. 98. 145-149 (2000)
Description
「研究成果報告書概要(和文)」より
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[Publications] Inoue,T.,Tanaka,T.,Takeichi,M.,Chisaka,O.,Nakamura,S.,Osumi,N.: "The role of cadherins in maintaining the compartment boundary between the cortex and striatum during development."Development. 128. 561-569 (2000)
Description
「研究成果報告書概要(和文)」より
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[Publications] Kawano, H., Fukuda, T., Kubo, K., Horie, M., Takeuchi, K., Osumi, N., Eto, K., and Kawamura, K.: "Pax6 is required for the thalamocortical pathway formation in fetal rats."J.Comp.Neurol.. 408. 147-60 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Shimoda, Y., Tajima, Y., Nagase, T., Harii K., Osumi, N., and Sanai, Y.: "Cloning and expression of a novel galactoside b1,3-glucuronyltransferase involved in the biosynthesis of HNK-1 epitope."J.Biol.Chem.. 274. 17115-17122 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Akamatsu, W., Okano, H.J., Osumi, N., Inoue, T., Nakamura, S., Sakakibara, S., Miura, M., Matsuo, N., Darnell, R.B.and Okano, H.: "Mammalian ELAV-like neuronal RNA-binding proteins HuB and HuC promote neuronal development in both the central and the peripheral nervous systems."Proc Natl Acad Sci U S A.. 96(17). 9885-90 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Tomioka, N., Osumi, N., Sato, Y., Inoue, T., Nakamura, S., Fujisawa, H., and Hirata, T.: "Neocortical origin and tangenitial migration of guidepost neurons In the lateral olfactory tract."J.Neurosci.. 20(15). 5802-5812 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yamamoto, H., Ochiya, T., Takahama, Y., Ishii, Y., Osumi, N., Sakamoto, H., Terada, M.: "Detection of spatial localization of Hst-Fgf-4 gene expression in brain and testis from adult mice."Oncogene. 19. 3805-3810 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Suzuki, R., Shintani, T., Sakuta, H., Kato, A., Ohkawara, T., Osumi, N., Noda, M: "Identification of RALDH-3, a novel retinaldehyde dehydrogenase, expressed in the ventral region of the retina."Mech Dev. 98. 37-50 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Nagase, T., Shimoda, Y., Sanai, Y., Nakamura, S., Harii, K., Osumi, N.: "Differential Expression of Two Glucuronyltransferases Synthesizing HNK-1 carbohydrate epitope in the sublineages of the rat myogenic progenitors."Mech Dev. 98. 145-149 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Inoue, T., Tanaka, T., Takeichi, M., Chisaka, O., Nakamura, S., & Osumi, N.: "The role of cadherins in maintaining the compartment boundary between the cortex and striatum during development."Development. 128. 561-569 (2001)
Description
「研究成果報告書概要(欧文)」より
