2000 Fiscal Year Final Research Report Summary
Anatomical analyses of phenotypes in mutant mice
| Project/Area Number |
11470004
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Hiroshima University |
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Principal Investigator |
YASUDA Mineo Hiroshima Univ., Fac.Med., Dept.Anat., Prof., 医学部, 教授 (50079688)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUI Kohji Hiroshima Univ., Fac.Med., Dept.Anat., Assistant, 医学部, 助手 (80183945)
YAMASHITA Keisuke Hiroshima Univ., Fac.Med., Dept.Anat., Assoc.Prof., 医学部, 助教授 (40166666)
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| Project Period (FY) |
1999 – 2000
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| Keywords | mutant mice / knock-out mice / Ahr gene / phenotypes / meromelia mice / trisomy 16 mice / trisomy 14 mice / hairpin tail mice |
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| Research Abstract |
This study aimed at analysing phenotypes in mutant mice anatomically by using various morphological methods in order to gain insight into actions of genes. In 1999 we analysed phenotypes in aryl hydrocarbon receptor (AhR or dioxin receptor) knock-out mice. In addition, in 2000 we made observations on phenotypes in trisomy 16 mice, trisomy 14 mice, and hairpin tail mice. In Ahr-/- mice anomalous vascular development and disturbed lobular patters were detected in the liver, suggesting a role of Ahr in vasculogenesis. In men/men mice, no obvious anomalies were detected other than limbs, hence the men gene seems to function limb-specifically. Introduction of the mutant men gene in two inbred strains is in progress to obtain congenic strains. Trisomy 16 mice are model animals for human trisomy 21 (Down's syndrome) arn have cleft palate in a high frequency. Patterns of palatal rugae in this mutant were severely abnormal even in fetuses without cleft palate. Ruga anomalies were also detected in trisomy 14 mice which exhibit various cardio-vascular anomalies, and in hairpin tail mice which have vertebral anomalies and bent tails. These findings indicate that palatal rugae are a good indicator of abnormnal palatogenesis.
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