• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2000 Fiscal Year Final Research Report Summary

Regulation of endothelial cell behavior by dystroglycan in neovascularization

Research Project

Project/Area Number 11470028
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field General pharmacology
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

KANOH Yumiko  Department of Structural Analysis, Senior Staff, 循環器形態部, 室長 (30214498)

Project Period (FY) 1999 – 2000
KeywordsLaminin / Fibronectin / Endothelial cell / Dystroglycan / Integrin / Neovascularization / Cytoskeleton
Research Abstract

We investigated a role of dystroglycan in the control of endothelial cell behavior in angiogenetic processes. The binding between endothelial cells and laminin-10 and -11 was dependent on endothelial dystroglycan, and was inhibited by heparin sulfate. It is well known that endothelial cells undergo migration, proliferation and tube formation in angeogenesis. All of these processes was regulated by dystroglycan. Expression of dystroglycan by endothelilal cell was cell cycle-dependent. The expression level was very low in G0 phase, in contrast to the high level expression in G2/M and S phases. Thus, endothelial expression of dystroglycan is under dynamic regulation and the expressed dystroglycan is involved in the control of cell behaviors.
Histochemical analysis showed that expression of dystroglycan was barely detectable both in the arteries and veins in the normal tissues whereas it was expressed at a high level in newly synthesized blood vessels in cancer tissues. To explore dystroglycan-dependent intracellular signal transduction cascade, we searched for a binding protein which associated with the intracellular domain of dystroglycan, and identified MAGI-1 as a candidate interactor of dystroglycan. MAGI-1 has two WW-domains and five PDZ domains. MAGI-1 bound to dystrolgycan by its WW-domains. MAGI-1 also bound to beta catenin by its fifth PDZ domain. Binding between MAGI-1 and beta catenin inhibited beta catenin degradation, and activated beta catenin - TCF/LEF1 cascade. These results indicated the presence of a signaling cascade between dystroglycan and the beta ctenin/TCF/LEF1 system linked by MAGI-1.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] H.Shimizu: "Adhesion of cultured bovine aortic endothelial cells to laminin-1 mediated by dystroglycan"J.Biol.Chem.. 274. 11995-12000 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Hosokawa: "Neuron specific expression of cationic amino acid transporter 3 in the adult rat brain"Brain.Res.. 838. 158-165 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Kanoh: "Lateral zone of cell-cell adhesion as the major fluid shear stress related signal transduction site"Circulation Res.,. 86. 425-433 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Okamoto: "Cholesterol oxidation switches the internalization pathway of ensothelin receptor type A from caveolae to clathrin coated pit"J.Biol.Chem.. 275. 6439-6446 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Kakutani: "A platelet-endothelium interaction mediated by lectin-like oxidized low-density lipoprotein receptor-1"Proc.Natl.Acad Sci.USA. 97. 360-364 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Katoh: "Stress fiber organization regulated by MLCK and Rho-kinase in cultured human fibroblasts."Am.J.Physiol.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Katoh: "Methods in Enzymology, vol.325, Regulators and effectors of small GTPases"Academic Press. 12 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Shimizu et al.: "Adhesion of cultured bovine aortic endothelial cells to laminin-1 mediated by dystroglycan"J.Biol. Chem.. 274. 11995-12000 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Hosokawa et al.: "Neuron specific expression of cationic amino acid transporter 3 in the adult rat brain"Brian. Res.. 838. 158-165 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Kanoh et al.: "Lateral zone of cell-cell adhesion as the major fluid shear stress related signal transduction site"Circulation Res. 86. 425-433 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Okamoto et al: "Cholesterol oxidation switches the internalization pathway of ensothelin receptor type A fromvaveolae to cleathin coated pit"J.Biol. Chem.. 275. 6439-6446 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Kakutani et al: "A platelet-endothelium interaction mediated by lectin-like oxidized low-density lipoprotein receptor-1"Proc. Natl. Acad. Sci., U.S.A.. 97. 360-364 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Kakutani et al: "A platelet-endothelium interaction mediated by lectin-like oxidized low-density lipporoten receptor-1"Proc. Natl. Acad. Sci., U.S.A./ t97. 360. 360-364 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Katoh et al.: "Isolation and in vitro contraction of stress fibers. In : W.E.Balch, C.J.Der and A.Hall eds. Regulators and effectors of small GTPases Part D : Rho family"Methods in Enzymology. Academic Press. 325. 369-380 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Katoh et al.: "Stree fiber organization regulated by MLCK and Rho-kinase in cultured human fibroblasts"Am. J.Physiol.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2002-03-26  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi